Genomics-based hypothesis generation: a novel approach to unravelling drug resistance in brain tumours?

Molecular evidence and functional expression of a novel drug efflux pump (ABCC2) in human corneal epithelium and rabbit cornea and its role in ocular drug efflux

“…These primers correspond to the nucleotide position 3537-3808. ABCC1 primers were designed from human MRP1 cDNA (Genbank Accession No: NM_004996) The forward and reverse primers designed for ABCC1 were 5…”

“…MRP's can confer drug resistance and cause reduced accumulation of therapeutic agents. A reduction in intracellular drug concentrations was found to correlate with the expression of Pgp encoded by multidrug resistance (MDR1) gene and a transmembrane phosphoglycoprotein (MRP) (Bredel et al, 2004;Sawicka et al, 2004). Also, collective role of P-gp and MRPs in efflux of drug molecules has been reported (Xiao et al, 2005).…”

Genetic studies of diseases

“…Such networks built during clinical and preclinical studies can also be used to monitor patients undergoing treatment after the clinical trial. These will be extremely useful for monitoring patients undergoing customized drug treatment in the future [59,62,63].…”

Interpreting Microarray Data and Related Applications Using Nonlinear System Identification

“…Bredel et al [66] have comprehensively reviewed the use of gene expression profiling in human brain tumors and pointed out that drug resistance here is likely to involve a complex network of regulatory dynamics. Recently, Pomeroy et al [61] showed that a variety of classification techniques, including weighted voting (WV), k-nearest neighbors (k-NN), support vector machines (SVMs), and IBM SPLASH could be used to predict clinical outcome of a group of 60 medulloblastoma patients from their gene expression profiles.…”