Characterization of calcium oscillation patterns in caprine oocytes induced by IVF or an activation technique used in nuclear transfer

Jellerette, Teru; Melican, D.; Butler, R.; Nims, S.; Ziomek, Carol A.; Fissore, Rafael A.; Gavin, W.

doi:10.1016/j.theriogenology.2005.08.025

Cited by 14 publications

(6 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two ejaculates from each of the remaining goats were extended in Tris-based extender containing 20% egg yolk and 7% glycerol, shipped cooled to GTC Biotherapeutics (Framingham, MA, USA), and used for IVF. IVF was performed as described previously (29), using a total of 687 in vivo or in vitro matured goat oocytes. The resulting embryos were cultured for 4 to 7 days and analyzed by single-embryo PCR.…”

Section: Experiments 3: Aav-mediated Goat Germ Cell Transduction and Tmentioning

confidence: 99%

Adeno‐associated virus (AAV)‐mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation

et al. 2007

View full text Add to dashboard Cite

We explored whether exposure of mammalian germ line stem cells to adeno-associated virus (AAV), a gene therapy vector, would lead to stable transduction and transgene transmission. Mouse germ cells harvested from experimentally induced cryptorchid donor testes were exposed in vitro to AAV vectors carrying a GFP transgene and transplanted to germ cell-depleted syngeneic recipient testes, resulting in colonization of the recipient testes by transgenic donor cells. Mating of recipient males to wild-type females yielded 10% transgenic offspring. To broaden the approach to nonrodent species, AAV-transduced germ cells from goats were transplanted to recipient males in which endogenous germ cells had been depleted by fractionated testicular irradiation. Transgenic germ cells colonized recipient testes and produced transgenic sperm. When semen was used for in vitro fertilization (IVF), 10% of embryos were transgenic. Here, we report for the first time that AAV-mediated transduction of mammalian germ cells leads to transmission of the transgene through the male germ line. Equally important, this is also the first report of transgenesis via germ cell transplantation in a nonrodent species, a promising approach to generate transgenic large animal models for biomedical research.

show abstract

Section: Experiments 3: Aav-mediated Goat Germ Cell Transduction and Tmentioning

confidence: 99%

Adeno‐associated virus (AAV)‐mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Exposure to calcium ionophore induces one high-amplitude calcium spike in oocytes (Rinaudo et al 1997, Tomashov-Matar et al 2005, Jellerette et al 2006. This may be sufficient to enable exit from meiotic arrest, via destruction of maturation promoting factor and other factors, especially if oocytes are subsequently cultured in the presence of 6-dimethylaminopurine (6-DMAP), a protein kinase inhibitor.…”

Section: Introductionmentioning

confidence: 99%

Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin

Hinrichs

Choi²,

Varner³

et al. 2007

Reproduction

View full text Add to dashboard Cite

We evaluated the effect of different activation treatments on the production of blastocysts and foals by nuclear transfer. Donor cells were prepared using roscovitine treatment, which has previously been associated with increased production of viable offspring. All activation treatments were followed by culture in 6-dimethylaminopurine (6-DMAP) for 4 h. In experiment 1, blastocyst production after activation by injection of sperm extract followed by treatment with ionomycin was significantly higher than that for activation with a serial treatment of ionomycin, 6-DMAP, and ionomycin (12.5 vs 2.8%; P!0.05) and tended to be higher than that for injection of sperm extract alone (3.4%; PZ0.07). In experiment 2, there were no significant differences in blastocyst development among treatments with ionomycin once or twice, sperm extract then ionomycin, or ionomycin then sperm extract (range 4.6-7.3%). Overall, transfer of 26 blastocysts resulted in 16 pregnancies (62%) and 9 live foals (35% of transferred embryos). Treatment with sperm extract followed by ionomycin produced a live foal rate per embryo transferred of 5/10 (50%). One foal died of pneumonia 48 h post partum and one foal died at 1 week of age after complications during induction of anesthesia; the remaining seven foals are currently 10-14 months of age.

show abstract

“…Other difference between IVF and parthenogenetic embryos is the calcium releasing at oocyte activation. While the chemical activation with ionomycin induces a single and large calcium release (Alberio, Zakhartchenko, Motlik, & Wolf, ; Jellerette et al, ), the fertilization sperm factors trigger a series of calcium oscillations (Alberio et al, ; Miyazaki & Ito, ), which effect is mediated by the oocyte machinery (Alberio et al, ; Tang et al, ). Also, nuclei of fertilized one‐ and two‐cell embryos have calcium‐releasing activity provided by the sperm, in contrast to parthenogenetic embryos (Kono, Carroll, Swann, & Whittingham, ).…”

Section: Discussionmentioning

confidence: 99%

Contrasting effects of heat shock during in vitro maturation on development of in vitro‐fertilized and parthenogenetic bovine embryos

Camargo

Costa

Munk

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus–oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleavage at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes.

show abstract

Characterization of calcium oscillation patterns in caprine oocytes induced by IVF or an activation technique used in nuclear transfer

Cited by 14 publications

References 37 publications

Adeno‐associated virus (AAV)‐mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation

Adeno‐associated virus (AAV)‐mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation

Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin

Contrasting effects of heat shock during in vitro maturation on development of in vitro‐fertilized and parthenogenetic bovine embryos

Contact Info

Product

Resources

About