Characterization of Ca2+ signaling pathways in human mesenchymal stem cells

Kawano, Seiko; Shoji, Shuichi; Ichinose, Shizuko; Yamagata, Kazuo; Tagami, Motoki; Hiraoka, Masayasu

doi:10.1016/s0143416002001240

Cited by 148 publications

(187 citation statements)

References 24 publications

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“…These results are in a perfect agreement with what has been observed in MSCs [19,23,24] and also in ESCs [19], suggesting that this might be a universal mechanism in the generation of Ca 2+ oscillations in stem cells. Moreover, we provided evidence that SOCE was also essential for Ca 2+ oscillations in CPCs.…”

Section: Functional Characterisation Of Internal Ca 2+ Stores and Thesupporting

confidence: 90%

“…Nevertheless, the chondrogenic potential of these cells has been demonstrated [25], and here we present data that the mRNA expression of various osteochondrogenic lineage markers was detectable in CPCs. In contrast to embryonic chicken limb bud-derived chondrogenic cells where high-frequency (8×10 -2 Hz) Ca 2+ oscillations could be detected during fluorescent single-cell Ca 2+ measurements [11,53], CPCs exhibited less frequent (1.3×10 -3 Hz) periodic increases in [Ca 2+ ]i, similar to what has been described in MSCs [24,50].…”

Section: Dynamics Of Cytosolic [Ca 2+ ]Isupporting

confidence: 66%

“…Ca 2+ oscillations have been reported in a great variety of non-excitable cells including pancreatic β-cells [49], embryonic stem cells (ESCs) [47], chondroblasts [11,35], chondrocytes [40] and human MSCs [22,24]. This is the first study to report that human chondroprogenitor cells derived from regions with minor lesions of osteoarthritic articular cartilage (CPCs) are also characterised by a similarly dynamic Ca 2+ homeostasis.…”

Section: Dynamics Of Cytosolic [Ca 2+ ]Imentioning

confidence: 98%

“…The aspecific IP3R inhibitor 2-aminoethoxydiphenyl borate (2-APB) [2] (75 µM) was diluted in 0 mM Ca 2+ Tyrode's (stock: 1 M in DMSO); the non-specific TRPC antagonist YM-58483 (a pyrazole derivative; also known as BTP-2) [15] (1 M) and the aspecific Ca 2+ influx channel blocker LaCl3 [24] (500 M) were diluted in normal (1.8 mM Ca 2+ ) Tyrode's solution (stocks: 1 mM and 300 mM in distilled water and DMSO, respectively). The sarcoplasmic/endoplasmic reticulum Ca 2+ -ATPase (SERCA) inhibitor cyclopiazonic acid (CPA; 10 µM) [27] was diluted in Ca 2+ -free Tyrode's solution (stock: 10 mM in DMSO).…”

Section: Solutions and Drugsmentioning

confidence: 99%

“…In terms of the two major Ca 2+ release channels of the ER, CPCs share homology with MSCs wherein all IP3R subtypes but no RyR isoforms are expressed [24,50], as opposed to chicken limb bud-derived chondroprogenitor cells wherein only IP3R1 could be detected [33]. ESCs, on the other hand, are known to express all three IP3R subtypes [19], as well as RyRs [47].…”

Section: Functional Characterisation Of Internal Ca 2+ Stores and Thementioning

confidence: 99%

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Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

Matta

Fodor

Miosge

et al. 2014

Pflugers Arch - Eur J Physiol

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Osteoarthritis (OA) is the most common form of chronic musculoskeletal disorders. A migratory stem cell population termed chondrogenic progenitor cells (CPC) with in vitro chondrogenic potential was previously isolated from OA cartilage. Since intracellular Ca 2+ signalling is an important regulator of chondrogenesis, we aimed to provide a detailed understanding of the Ca 2+ homeostasis of CPCs. In this work, CPCs immortalised by lentiviral administration of the human telomerase reverse transcriptase (hTERT) and grown in monolayer cultures were studied. Expressions of all three IP3Rs were confirmed, but no RyR subtypes were detected. Ca 2+ oscillations observed in CPCs were predominantly dependent on Ca 2+ release and store replenishment via store-operated Ca 2+ entry; CPCs express both STIM1 and Orai1 proteins. Expressions of adenosine receptor mRNAs were verified, and adenosine elicited Ca 2+ transients. Various P2 receptor subtypes were identified; P2Y1 can bind ADP; P2Y4 is targeted by UTP; and ATP may evoke Ca 2+ transients via detected P2X subtypes, as well as P2Y1 and P2Y2. Enzymatic breakdown of extracellular nucleotides by apyrase completely abrogated Ca 2+ oscillations, suggesting that an autocrine/paracrine purinergic mechanism may drive Ca 2+ oscillations in these cells.As CPCs possess a broad spectrum of functional molecular elements of Ca 2+ signalling, Ca 2+ -dependent regulatory mechanisms can be supposed to influence their differentiation potential.

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Section: Functional Characterisation Of Internal Ca 2+ Stores and Thesupporting

confidence: 90%

Section: Dynamics Of Cytosolic [Ca 2+ ]Isupporting

confidence: 66%

Section: Dynamics Of Cytosolic [Ca 2+ ]Imentioning

confidence: 98%

Section: Solutions and Drugsmentioning

confidence: 99%

Section: Functional Characterisation Of Internal Ca 2+ Stores and Thementioning

confidence: 99%

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Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

Matta

Fodor

Miosge

et al. 2014

Pflugers Arch - Eur J Physiol

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Ca²⁺‐depended signaling pathways regulate self‐renewal and pluripotency of stem cells

Ермаков

Daks

Fedorova

et al. 2018

Cell Biology International

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Ca -mediated signaling is widely spread in nature and plays critical role in the individual development of various organisms ranging from microorganisms to mammals. In vertebrates, Ca is involved in important developmental events: fertilization, body plan establishment, and organogenesis. The two later events are defined by embryonic stem cells (ESCs). ESCs are capable of self-renewal and are pluripotent by nature, that is, can give rise to all types of cells that make up the body. Given the paramount importance of Ca signalization in the development, it is therefore not surprising this process also plays role in the biology of stem cells. In this review, we scrutinize the published experimental data on the role of Ca ions in embryonic stem cells self-renewal and pluripotency. In line with this, we also discuss possible mechanisms of p53 inhibition as a major hindrance to self-renewal of ESCs. Finally, we argue about the role of G-protein-coupled receptors (GPCRs), the largest family of heteromeric transmembrane receptors, and GPCR-mediated signalization in stem cells, and propose the role for the GPCR-G-protein-PLC-Ca -downstream signaling pathway in the regulation of pluripotency of both mouse and human ESCs.

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Distal C‐terminus of Ca_v1.2 is indispensable for the chondrogenic differentiation of rat dental pulp stem cells

Ren

Zhao

2019

Cell Biology International

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The α1 subunit (Cav1.2) of the L‐type calcium channel (LTCC), which is presently existing in both excitatory cells and non‐excitatory cells, is involved in the differentiation and proliferation of mesenchymal stem cells (MSCs). Dental pulp stem cells (DPSCs), MSCs derived from dental pulp, exhibit multipotent characteristics similar to those of MSCs. The aim of the present study was to examine the contribution of Cav1.2 and its distal C‐terminus (DCT) to the commitment of rat DPSCs (rDPSCs) toward chondrocytes and adipocytes in vitro. The expression of Cav1.2 was obviously elevated in chondrogenic differentiation but did not differ significantly in adipogenic differentiation. The chondrogenic differentiation but not adipogenic of rDPSCs was inhibited by either blocking LTCC using nimodipine or knockdown of Cav1.2 via short hairpin RNA (shRNA). Overexpression of DCT rescued the inhibition by Cav1.2‐shRNA during chondrogenic differentiation, indicating that DCT is essential for the chondrogenic differentiation of rDPSCs. However, the protein level of DCT decreased after chondrogenic differentiation in wild‐type cells, and overexpression of DCT in rDPSCs inhibited the phenotype. These data suggest that DCT is indispensable for chondrogenic differentiation of rDPSCs but that superfluous DCT inhibits this process. Through the analysis of differentially expressed genes using RNA‐seq data, we speculated that the regulation of DCT might be mediated by the mitogen‐activated protein kinase/extracellular‐regulated kinase and c‐Jun N‐terminal kinase signaling pathways, or Chondromodulin‐1.

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Characterization of Ca2+ signaling pathways in human mesenchymal stem cells

Cited by 148 publications

References 24 publications

Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

Ca²⁺‐depended signaling pathways regulate self‐renewal and pluripotency of stem cells

Distal C‐terminus of Ca_v1.2 is indispensable for the chondrogenic differentiation of rat dental pulp stem cells

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Characterization of Ca2+ signaling pathways in human mesenchymal stem cells

Cited by 148 publications

References 24 publications

Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

Ca2+‐depended signaling pathways regulate self‐renewal and pluripotency of stem cells

Distal C‐terminus of Cav1.2 is indispensable for the chondrogenic differentiation of rat dental pulp stem cells

Contact Info

Product

Resources

About

Ca²⁺‐depended signaling pathways regulate self‐renewal and pluripotency of stem cells

Distal C‐terminus of Ca_v1.2 is indispensable for the chondrogenic differentiation of rat dental pulp stem cells