Characterization of Brazilian soybean cultivars using microsatellite markers

Priolli, Regina Helena Geribello; Mendes-Junior, Celso Teixeira; Arantes, N. E.; Contel, E. P. B.

doi:10.1590/s1415-47572002000200012

Cited by 71 publications

(86 citation statements)

References 13 publications

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“…High levels of polymorphism were revealed in soybean by the use of neutral SSR markers, for both the number of alleles per locus and gene diversity (Akkaya et al, 1992;Narvel et al, 2000;Priolli et al, 2002;Priolli et al, 2010). In this study, using SSR markers and considering the parameters of BNF and protein content, we have also observed high variability amongst both modern germoplasms cultivated in Brazil and ancestral lines.…”

Section: Discussionsupporting

confidence: 56%

Genetic structure and diversity of a soybean germplasm considering biological nitrogen fixation and protein content

Grunvald¹,

Martins²,

Santos³

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Biological nitrogen fixation (BNF) has global economic and environmental importance, but has often not been considered in soybean [Glycine max (L.) Merrill] breeding programs. Knowing the genetic diversity and structure of a population within a germoplasm represent a key step for breeding programs. This study aimed at determining the structure of the population and diversity of soybean with regard to BNF and protein content in grain. In total, There is variability for BNF and protein content amongst both modern germoplasms cultivated in Brazil and ancestral lines. This variability could be better explored in soybean breeding programs to improve these traits.

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Section: Discussionsupporting

confidence: 56%

Genetic structure and diversity of a soybean germplasm considering biological nitrogen fixation and protein content

Grunvald¹,

Martins²,

Santos³

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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“…O polimorfismo deste conjunto de locos microssatélites já havia sido observado na determinação de perfis neste grupo de cultivares (Priolli et al, 2002). Entretanto, Alcantara Neto (2001) não observou polimorfismo em três dos 12 locos aqui estudados (Satt002, Satt005 e Satt009) em um grupo de 32 cultivares brasileiras.…”

Section: Resultsunclassified

“…Goiana e Indusem tiveram, respectivamente, 11, 9 e 8 cultivares analisadas, número inferior ao do grupo IAC. Entretanto, uma análise mais detalhada da genealogia deste grupo de cultivares (Priolli et al, 2002) revelou que 12 entre as 18 cultivares descendem das cultivares Paraná e IAC 8, sendo que estas descendem da cultivar Hill. Deste modo, com a maior endogamia, não chegou a ser surpreendente que existisse maior heterogeneidade (ou estruturação) neste programa, em relação aos demais.…”

Section: Resultsunclassified

Diversidade genética da soja entre períodos e entre programas de melhoramento no Brasil

Priolli

Mendes-Junior

Sousa

et al. 2004

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi detectar os efeitos do melhoramento sobre a diversidade do germoplasma da soja cultivada nas três ultimas décadas, por meio da comparação de seis programas de melhoramento e períodos de lançamento de cultivares, utilizando locos microssatélites. Em relação aos programas de melhoramento, todos os locos apresentaram diferenças significativas em suas distribuições alélicas. Alguns locos eram compostos de alelos exclusivos em alguns programas de melhoramento, enquanto outros foram compostos sempre dos mesmos alelos em maior freqüência para todos os programas. A AMOVA indicou maior porção da variância devido a cultivares dentro de programas e somente 5,3% (p<0,05) devido à diferença entre programas. Quando comparados os programas de melhoramento entre si, cinco entre as 15 comparações apresentaram diferenças significativas (p<0,05), estando presente o programa IAC em quatro destas cinco comparações. As estimativas de variabilidade da soja entre os períodos de melhoramento avaliados indicaram que somente 1,78% da variância total foi devida à diferença entre períodos (p>0,05). Os resultados sugerem que o germoplasma de soja utilizado em programas de melhoramento no Brasil manteve nível constante de diversidade genética nos últimos 30 anos, além de relativa heterogeneidade de determinados programas.Termos para indexação: Glycine max, SSR, germoplasma, erosão genética. Soybean genetic diversity in time and among breeding programs in BrazilAbstract -The objective of this work was to detect the effects of plant breeding over the genetic diversity of soybean germplasm grown during the last three decades by comparing six different breeding programs and releasing intervals of soybean cultivars, using microsatellite loci. Regarding the breeding programs, all loci presented significantly different allelic distributions. Some loci presented alleles restricted to unique breeding programs, while others had a same allele being the most frequent in all the six programs. The AMOVA revealed that the major proportion of the variance occurred among cultivars within breeding programs and only 5.3% (p<0.05) of the whole variance was due to differences among breeding programs. When the breeding programs were pairwise-compared, five out of the 15 comparisons showed significant differences (p<0.05), being the IAC program within four of them. Soybean variability estimates of the five different releasing intervals revealed that just 1.78% of the variance is due to differences among them (p>0.05). The results suggest that the soybean germplasm pool involved in breeding programs in Brazil has maintained a stable genetic diversity during the past 30 years, in addition to a relative heterogeneity of some breeding programs.

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“…Thus, microsatellite loci are often multi-allelic (Saghai-Maroof et al, 1994). In addition to high levels of polymorphism, SSR sequences possess most of the desirable attributes of molecular markers, including information content, unambiguous designation of alleles, neutral selectively (although they can be subjected to hitch-hiking effects), high reproducibility, codominance, and fast and easy assaying of genotypes and therefore microsatellite markers or SSR have proved to be very useful for cultivar identification, pedigree analysis and the evaluation of genetic distance between organisms (Priolli et al, 2002) and genetic mapping (Yu et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)

Yaish

Vega

2003

Genet. Mol. Biol.

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The isolation of (GA) n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA) n microsatellites with n values equal or higher than six. These (GA) n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA) n microsatellite linked to a putative MADS-box gene was identified. This sequence, which represents the first MADS-box locus described to date in common bean, showed a very high similarity with other known MADS-box sequences and was grouped within the AGL2 subfamily cluster of the Arabidopsis MADS-box genes. The vicinity of microsatellites to some genes is also discussed.

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Characterization of Brazilian soybean cultivars using microsatellite markers

Cited by 71 publications

References 13 publications

Genetic structure and diversity of a soybean germplasm considering biological nitrogen fixation and protein content

Genetic structure and diversity of a soybean germplasm considering biological nitrogen fixation and protein content

Diversidade genética da soja entre períodos e entre programas de melhoramento no Brasil

Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)

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