Characterization of brain acetylcholinesterase of bentonic fish Hoplosternum littorale: Perspectives of application in pesticides and metal ions biomonitoring

Araújo, Marlyete Chagas de; Assis, Caio Rodrigo Dias; Silva, Kaline Catiely Campos; Souza, Kelma Sirleide de; Azevedo, Rafael Souto; Alves, Maria Helena Menezes Estevam; Silva, Luciano Clemente; Silva, Valdir Luna; Adam, Mônica Lúcia; Bezerra, Ranilson S.; Oliveira, Maria Betânia Melo de

doi:10.1016/j.aquatox.2018.10.017

Cited by 20 publications

(9 citation statements)

References 52 publications

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“…It acts as an analog of juvenile insect hormone, a growth regulator [Ishaaya and Horowitz, 1992;World Health Organization (WHO), 2006;Chłopecka et al, 2018]. Pyriproxyfen may also act as an acetylcholinesterase inhibitor in fish (Araújo et al, 2018;Maharajan et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Gusso

Reolon

Gonzalez

et al. 2020

Front. Behav. Neurosci.

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Pyriproxyfen is one of the most used larvicides and insecticides; it acts as an analog of juvenile insect hormone (a growth regulator). It is highly toxic during all stages of mosquito development, suppresses metamorphosis, and interferes in insect reproduction and proliferation. Pyriproxyfen and its main metabolite have been shown to affect brain development in rodents. This compound is employed mainly to eliminate outbreaks of the genus Aedes, even in potable water. Despite the increasing number of toxicological studies about larvicides and insecticides-with an indication of continuous use-there have been few studies about the effects of pyriproxyfen in non-target species such as fish. This study evaluated the effects of pyriproxyfen on behavioral, cognitive, and endocrine parameters in zebrafish. We exposed adult zebrafish to different pyriproxyfen (Pestanal) concentrations (0.125, 0.675, and 1.75 mg/l) for 96 h. We analyzed behavioral parameters, memory, cortisol levels, and gene expression of glucocorticoid receptor (gr) and corticotrophin-releasing factor (crf) after pyriproxyfen exposure. This exposure did not alter locomotion (distance or mean speed), anxiety-like behavior (latency to enter to the top zone of the tank or time in the top zone of the tank), and social or aggressive behavior. However, there was impaired inhibitory avoidance memory at all tested pyriproxyfen concentrations. Cortisol levels were reduced in exposed groups when compared to control or vehicle. However, gr and crf gene expression in pyriproxyfen-treated animals were unaltered when compared to control or vehicle groups. Taken together, these findings indicate that pyriproxyfen may induce cognitive impairment and altered cortisol levels in zebrafish, a non-target species.

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Section: Introductionmentioning

confidence: 99%

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Gusso

Reolon

Gonzalez

et al. 2020

Front. Behav. Neurosci.

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“…vannamei and C. rhizophorae using the Ellman method with modifications . The studied drug solutions were serially diluted, with concentrations ranging from 0 to 300 μM.…”

Section: Methodsmentioning

confidence: 99%

“…The AchE inhibition studies were carried out with the commercial AChE E. electricus and the extracts obtained from L. vannamei and C. rhizophorae using the Ellman method with modifications. 29 The studied drug solutions were serially diluted, with concentrations ranging from 0 to 300 μM. In a 96-well plate, 10 μL of the compounds was incubated at varying concentrations with 10 μL of the enzyme (1 μg/mL) at 298 K for 1 h. After that time, 200 μL of DTNB (0.25 mM) and 20 μL of the acetylthiocholine iodate substrate (62 mM) were added to each well of the plate to record the absorbance at 405 nm, in order to identify the product formed from the reaction between DTNB and the substrate used.…”

Section: Methodsmentioning

confidence: 99%

Binding Mechanism between Acetylcholinesterase and Drugs Pazopanib and Lapatinib: Biochemical and Biophysical Studies

Menezes

Assis

Cintra

et al. 2021

ACS Chem. Neurosci.

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Tyrosine kinase inhibitors (TKIs) are antitumor compounds that prevent the phosphorylation of proteins in a biological environment. However, the multitarget performance of TKIs promotes them as possible candidates for drug repositioning. In this work, interaction and inhibition studies through spectroscopic and computational techniques to evaluate the binding effectiveness of lapatinib and pazopanib TKIs to acetylcholinesterase (AChE) are reported. The results indicated potent inhibition at the μM level. The types of inhibition were identified, with pazopanib acting through non-competitive inhibition and lapatinib through acompetitive inhibition. The fluorescence suppression studies indicate a static mechanism for lapatinib−AChE and pazopanib−AChE systems, with a binding constant in the order of 10 5 M −1 . The obtained thermodynamic parameters reveal interactions driven by van der Waals forces and hydrogen bonds in the lapatinib−AChE system (ΔH°and ΔS°< 0). In contrast, the pazopanib−AChE system shows positive ΔH°and ΔS°, characteristic of hydrophobic interactions. The Foster resonance energy transfer study supports the fluorescence studies performed. The 3D fluorescence studies suggest changes in the microenvironment of the tryptophan and tyrosine residues of the protein in contact with lapatinib and pazopanib. The results suggest effective inhibition and moderate interaction of the drugs with AChE, making them interesting for conducting more in-depth repositioning studies as AChE inhibitors.

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“…Recently, benzoylurea, a class of pesticide that in the past was not considered an acetylcholinesterase inhibitor, since its main mode of action is the inhibition of chitin biosynthesis in insects (which interrupts the incorporation of N-acetylglycosamine monomers), demonstrated anticholinesterase potential [19]. In 2011, this class of pesticides represented 3.6% of the world's pesticide market.…”

Section: Figure 2 Organophosphate Pesticides: (A) Methyl-paration (Triesters Of Phosphoric Acid) (B) Carbamate Carbofuran (Esters Derivedmentioning

confidence: 99%

Impacts of Agricultural Toxicity on Non-Target Organisms in Aquatic Ecosystem

Araújo¹,

Assis²,

Sá³

et al. 2021

Emerging Contaminants

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The present review addresses the impacts of pesticides used in crops on non-target organisms in aquatic ecosystems. In recent decades, these ecosystems have received large amounts of these compounds, which are released by urban communities, rural and industrial properties. Pesticides reach the aquatic environment through different routes (leaching, irrigation, drainage, and surface runoff) and can easily reach non-target organisms, such as fish, mollusks, as well as other benthic organisms. Usually, these animals tend to undergo bioaccumulation. Exposure to these pesticides can cause numerous physiological changes by direct influence on certain cellular structures, such as on the lysosomal membrane, which can be degraded. Also, they can even react with nucleic acids resulting in several genetic injuries, thus causing adverse reactions to the body. There is a need for more incentives for the adoption of sustainable agroecological practices, as well as a ban on active ingredients harmful to the environment, in addition to strict inspection by competent environmental agencies.

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Characterization of brain acetylcholinesterase of bentonic fish Hoplosternum littorale: Perspectives of application in pesticides and metal ions biomonitoring

Cited by 20 publications

References 52 publications

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Binding Mechanism between Acetylcholinesterase and Drugs Pazopanib and Lapatinib: Biochemical and Biophysical Studies

Impacts of Agricultural Toxicity on Non-Target Organisms in Aquatic Ecosystem

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