Characterization of Bovidae sex-determining gene SRY

Cheng, Hanhua; Shi, Huiping; Zhou, Rongjia; Guo, Yiqing; Liu, Li; Liu, Jiangdong; Jiang, Ya Dong; Kudo, Toshiyuki; Sutou, Shizuyo

doi:10.1186/1297-9686-33-6-687

Cited by 12 publications

(12 citation statements)

References 19 publications

(10 reference statements)

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“…In the same way the carboxy-terminus is much more similar to the C terminal end of most mammals than mouse Sry protein due to a large poly Gln/His transactivation domain ( fig. 5 ) [Carlisle et al, 1996;Daneau et al, 1996;Cheng et al, 2001;Moreira, 2002;Nishida et al, 2003;Geraldes et al, 2005;King et al, 2007].…”

Section: The Sry Gene In European Arvicolidsmentioning

confidence: 99%

Sequence Analysis and Mapping of the <i>Sry </i>Gene in Species of the Subfamily Arvicolinae (Rodentia)

Acosta

Marchal²,

Romero-Fernández³

et al. 2010

Sex Dev

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The rodent subfamily Arvicolinae, which contains about 125 species, presents some interesting exceptions concerning Sry, the sex determining gene in mammals. In some species multiple Sry copies have been described on the Y chromosome and in the Iberian vole, Microtus cabrerae, several Sry sequences have been cloned and mapped not only on the Y but also on the X chromosome. Here we present a comparative analysis of Sry sequences from a total of 22 species. Our study demonstrates for the first time that for most North American species, as previously reported for the European species, multiple copies of the Sry gene exist on the Y chromosome. Furthermore, we have sequenced and analyzed the full sequence of Sry from several European species, showing that the sequence and structure of the gene in this group of species present the main features described for Sry in other mammals. Finally, FISH analyses on some of these species demonstrated that all Sry sequences, despite their functional status, mapped on the euchromatic short arm of the Y chromosome.

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Section: The Sry Gene In European Arvicolidsmentioning

confidence: 99%

Sequence Analysis and Mapping of the <i>Sry </i>Gene in Species of the Subfamily Arvicolinae (Rodentia)

Acosta

Marchal²,

Romero-Fernández³

et al. 2010

Sex Dev

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“…Clinical case of triplet ending with live calves was very low less than 0.6% [10]. In the normal male, Y chromosome-linked SRY gene is responsible for male sex determination [2,11], whereas in the XY female, mutation of SRY gene is detected [5][6][7]. Therefore, Case 2 which showed only XY chromosome with female like genitalia, however, possession of testis and detection of SRY gene was implied that Case 2 was not a case of XY female.…”

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confidence: 99%

“…, Mineto TANI 3) , Kazumi NIBE 4) , Go KITAHARA 2) , Shingo HANEDA 5) , Motozumi MATSUI 5) , Yoh-Iichi MIYAKE 5) and Shunichi KAMIMURA 2) Abnormal development of the reproductive organs occurs with different incidence in several species [11,17]. A male or a female pseudohermaphrodite (PH) depends upon the nature of the gonadal tissues.…”

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confidence: 99%

Two Cases of Bovine Male Pseudohermaphrodites with Different Endocrinological and Pathological Findings

Moriyama

Tani²,

Nibe

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. Two cases of bovine male pseudohermaphrodites (PH) were subjected for clinical investigation with transrectal ultrasonography, endocrinology with adoption of hCG-stimulation test, cytogenetics with analysis of sex chromosome and Y-specific DNA, and finally histological examination. Results were compared with normal calves. Case 1 was a 10-month-old calf with XX/XY chimeras, showing elevation of testosterone (T) levels, but no change in progesterone (P 4 ) after hCG test, and possessed atrophied testes in the cavitas pelvis. Case 2 was an 18-month-old calf with SRY positive-XY chromosome, showing lower level of plasma T and P 4 after hCG test, and possessed atrophied testes and undifferentiated genital ducts. Both cases possessed female-like external genitalia with similar pathological findings, however endocrinological and cytogenetical aspects were different each other.KEY WORDS: atrophied testis, hCG stimulation test, male pseudohermaphrodite, sex chromosome, ultrasonography.J. Vet. Med. Sci. 72(4): 507-510, 2010 Abnormal development of the reproductive organs occurs with different incidence in several species [11,17]. A male or a female pseudohermaphrodite (PH) depends upon the nature of the gonadal tissues. PH has either a testis or an ovary, and possesses external genitalia resembling the opposite sex [8,9,12,15]. PH has been reported in several species, but sporadic in cattle. There are limited cases reports concerning bovine PH, however these often lack general investigation in endocrinological aspect for gonadal function, comparing external and internal genitalia using ultrasonography, or chromosomal examination [5,8,16]. In the present study, we investigated two cases of male PH, which had different nature of endocrinological, cytogenetical and pathophysiological findings. In comparison with PH cases for novel trial, normal male and female calves were examined in the same manner as controls on the stand point for clinical investigation.Case 1: A 10-month old Japanese brown was born to male calf co-twins, and possessed female-like external genitalia showing urination toward upper direction. Coat and skin in neck region seemed to be masculine as the animal had grown. The animal showed a 3 cm in length of labia-like orifice (not vagina), but neither uterus and ovary nor testes were detected in the cavitas pelvis by rectal palpation. In addition, spiral stick-like structure (4  3 cm ) beneath the anal orifice was observed with trasrectal ultrasonography equipped with a 7.5 MHz linear-array transducer (SonoSite Case 2: An 18-month-old Japanese black, which had normal female-like external genitalia, but lacked estrous behavior in life. The vestibule of vagina was 13 cm in length; however neither uterus nor ovary was detected with rectal palpation. With transrectal ultrasonography, a pair of testes like organs (5  3 cm) with mediastinum was depicted (Fig. 1c).In order to investigate endocrinological aspects, 3,000 IU of human chronic gonadotropin (hCG; Veterinary Puberogen ® , Sankyo, Tokyo, Ja...

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“…In primates (Whitfield et al 1993), rodents (Tucker and Lundrigan 1993), artiodactyls (Cheng et al 2001;Quilter et al 2002), and carnivores (Murphy et al 1999;Olivier et al 1999) SRY is a single-copy, intronless gene that encodes a protein with a conserved high mobility group (HMG) box. The HMG box is a transcription factor shared by autosomal paralogues in the SOX gene family.…”

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Evolution of the Male-Determining GeneSRYWithin the Cat Family Felidae

et al. 2007

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In most placental mammals, SRY is a single-copy gene located on the Y chromosome and is the trigger for male sex determination during embryonic development. Here, we present comparative genomic analyses of SRY (705 bp) along with the adjacent noncoding 59 flank (997 bp) and 39 flank (948 bp) in 36 species of the cat family Felidae. Phylogenetic analyses indicate that the noncoding genomic flanks and SRY closely track species divergence. However, several inconsistencies are observed in SRY. Overall, the gene exhibits purifying selection to maintain function (v ¼ 0.815) yet SRY is under positive selection in two of the eight felid lineages. SRY has low numbers of nucleotide substitutions, yet most encode amino acid changes between species, and four different species have significantly altered SRY due to insertion/ deletions. Moreover, fixation of nonsynonymous substitutions between sister taxa is not consistent and may occur rapidly, as in the case of domestic cat, or not at all over long periods of time, as observed within the Panthera lineage. The former resembles positive selection during speciation, and the latter purifying selection to maintain function. Thus, SRY evolution in cats likely reflects the different phylogeographic histories, selection pressures, and patterns of speciation in modern felids.

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Characterization of Bovidae sex-determining gene SRY

Cited by 12 publications

References 19 publications

Sequence Analysis and Mapping of the <i>Sry </i>Gene in Species of the Subfamily Arvicolinae (Rodentia)

Sequence Analysis and Mapping of the <i>Sry </i>Gene in Species of the Subfamily Arvicolinae (Rodentia)

Two Cases of Bovine Male Pseudohermaphrodites with Different Endocrinological and Pathological Findings

Evolution of the Male-Determining GeneSRYWithin the Cat Family Felidae

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