Characterization of biosurfactants produced by the oil-degrading bacterium Rhodococcus erythropolis S67 at low temperature

Luong, T. M.; Понаморева, О. Н.; Nechaeva, I. A.; Petrikov, Kirill; Delegan, Ya. A.; Surin, Alexey K.; Linklater, Denver P.; Филонов, А. Е.

doi:10.1007/s11274-017-2401-8

Cited by 40 publications

(21 citation statements)

References 38 publications

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“…The stability studies on glycolipid BS derived from P. aeruginosa HAK01 indicated that this BS was able to function under a wide range of temperatures (40-121°C) and pH (4-10) (Khademolhosseini et al 2019). Furthermore, an effective oil-degrading Rhodococcus erythropolis S67 produced glycolipid, which could function at a temperature as low as 10°C (Luong et al 2018). Our findings suggested that glycolipid BS was able to withstand extreme pH, as it is consistent with other reports.…”

Section: Discussionsupporting

confidence: 90%

Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

Ekprasert

Kanakai

Yosprasong

2020

Polish Journal of Microbiology

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This work aimed to optimize carbon and nitrogen sources for the growth of Enterobacter cloacae B14 and its biosurfactant (BS) production via One-Variable-At-a-Time (OVAT) method. The BS stability under a range of pH and temperatures was assessed. Antimicrobial activity against Gram-positive and Gram-negative pathogens was determined by the agar well diffusion method. The results showed that the optimum carbon and nitrogen sources for BS production were maltose and yeast extract, respectively, with a maximum BS yield of (39.8 ± 5.2) mg BS/g biomass. The highest emulsification activity (E24) was 79%, which is significantly higher than in the previous studies. We found that B14 BS can withstand a wide range of pH values from 2 to10. It could also function under a range of temperatures from 30–37°C. Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectrometry (FTIR) analysis confirmed that B14 BS is a glycolipid-like compound, which is rarely found in Enterobacter spp. Cell-free broth showed inhibition against various pathogens, preferable to Gram-positive ones. It had better antimicrobial activity against Bacillus subtilis than a commonly-used antibiotic, tetracycline. Furthermore, B14 broth could inhibit the growth of a tetracycline-resistant Serratia marcescens. Our results showed promising B14 BS applications not only for bioremediation but also for the production of antimicrobial products.

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Section: Discussionsupporting

confidence: 90%

Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

Ekprasert

Kanakai

Yosprasong

2020

Polish Journal of Microbiology

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“…Though R. erythropolis is known to produce biosurfactants (Cai et al, 2016;Pirog et al, 2013), most of those belong to low molecular weight glycolipids, for example trehalolipeids, decrease both surface and interfacial tension of different phases (Luong et al, 2018;Patil & Pratap, 2018). However, the compound released by R. erythropolis OSDS1 described in this study, belongs to the high molecular weight bioemulsifier (extracellular polymer), which forms stable emulsification without causing obvious reduction in surface tension.…”

Section: Resultsmentioning

confidence: 74%

Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Xia¹,

Chakraborty

et al. 2019

Bioresource Technology

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The aim of this work was to study the production of bioemulsifier by Rhodococcus erythropolis OSDS1, and the improvement of crude oil depletion efficiency using a consortium of petroleum hydrocarbon degraders and OSDS1. The results showed that R. erythropolis OSDS1 produced highly stable bioemulsifier under various salinity (0-35 g/L NaCl) and pH (5.0-9.0) conditions; more than 90% of the initial emulsification activity was retained after 168 h. Emulsification capacity of the bioemulsifier on different petroleum hydrocarbons was diesel > mineral oil/crude oil > gasoline. A mixed bacterial consortium combining OSDS1 and four other petroleum hydrocarbon degraders was constructed. GC-MS results revealed that the constructed consortium achieved 85.26% depletion efficiency of crude oil in 15 days, which was significantly higher than that of individual strains. During the process, alkane hydroxylase gene (alkB) was successfully amplified from the consortium, confirming presence of crude oil degrading enzymes.

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“…The annotated data also shows a high similarity with two other known genomes of R. erythropolis (PR4 and CCM2925) ( Table 1 ). Among the Rhodococci species, R. erythropolis is widely known in xenobiotic mineralisation as numerous industrially and environmentally important enzymatic reactions have been described in various strains [ 47 , 48 ]. A 16S rRNA gene sequence of approximately 1500 bp (retrieved via RNAmmer 1.2) was found to be 100% identical with R. erythropolis PR4 (NR_074622) [ 49 ] and Nocardia coeliaca DSM 44,595 (NR_104776) [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus

Habib

Ahmad

Johari

et al. 2020

IJMS

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Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain’s genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research.

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Characterization of biosurfactants produced by the oil-degrading bacterium Rhodococcus erythropolis S67 at low temperature

Cited by 40 publications

References 38 publications

Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus

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