Characterization of biogenic selenium nanoparticles derived from cell-free extracts of a novel yeast Magnusiomyces ingens

Abstract

A facile one-pot and effective green process for biogenic selenium nanoparticles (SeNPs) was obtained using the cellfree extracts of a novel yeast Magnusiomyces ingens LH-F1. The corresponding absorption peak of SeNPs was observed at ~ 560 nm by UV-vis spectrophotometer. In the present study, SeO 2 2 mM, protein 500 mg L −1 and pH 7 were preferable to the biosynthesis of SeNPs. The effects of pH, SeO 2 concentration and protein concentration on the synthesis process were different. Transmission electron microscopy image exhibited that all the SeNPs were spherical and quasi-spherical with the diameters mainly distributed in 70-90 nm (average particles size was 87.82 ± 2.71 nm). X-ray diffraction suggested that the nanoparticles were composed of standard hexagonal crystalline Se with high purity. Fourier transform infrared spectroscopy indicated that some biomolecules such as hydroxyl, carboxyl and amino groups in the yeast cell-free extracts might be involved in the formation of SeNPs. Analyses of sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that two proteins with low molecular weight approximately ~ 16 and ~ 21 kDa were detected on the surface of SeNPs and in the extracts, which could play the role of natural stabilizers and confer stability to synthesized SeNPs; whereas, unbound proteins on the SeNPs surface could act as reducing agents. Antibacterial analysis showed that the SeNPs could inhibit Arthrobacter sp. W1 (Gram positive) but not E. coli BL21 (Gram negative), which could provide reference for antimicrobial application of biogenic SeNPs.

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

“…For Magnusiomyces ingens LH-F1 (CGMCC No. 10367), the average particle size was 87.8 nm [62]. The formation of Se nanocolloids in cellular cytosol is associated with the detoxification process [16].…”

“…The production of SeNPs by microorganisms is a widespread phenomenon in nature, which has raised considerable interest in recent years [62]. In this context, the presence of Se-bearing NPs in the sample was investigated by single particle (SP)-ICP-MS and transmission electron microscopy (TEM).…”

“…Yeast reduces the soluble sodium selenite (Na 2 SeO 3 ) to a red elemental form (Se 0 ) [67]. According to Lian et al [62], SeNPs may contain some proteins and lipids on their surface, which further stabilize their structure. The formation of SeNPs in yeast grown at different concentrations of this element causes a change in the color of the biomass of cells from bright yellow at the beginning of incubation to orange or even red.…”

Green Synthesis of Gold and Silver Nanoparticles Using Leaf Extract of Clerodendrum inerme; Characterization, Antimicrobial, and Antioxidant Activities

“…have been exploited as antibacterial agents, targeted drug delivery vehicles, antimycotic agents, antioxidant agents, anticancer agents, etc. [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. Among them, Au and Ag NPs are of high significance due to their unique properties.…”

Green Synthesis of Selenium and Tellurium Nanoparticles: Current Trends, Biological Properties and Biomedical Applications

“…This efficient method enabled the selective identification and quantification of both the unreacted Se(IV) and the final water-soluble SeNPs without the need to separate them. Lian et al synthesized spherical and quasispherical SeNPs of 70-90 nm in size utilizing the yeast cell-free extract of Magnusiomyces ingens LH-F1; some surface proteins played a significant role during the synthesis, acting as reducing or capping agents [207]. Nevertheless, the mechanisms of SeNP formation are not fully understood.…”