Characterization of bifidobacteria by random DNA amplification

Vincent, Daniel; Roy, Denis; Mondou, Francine; Déry, Claude V.

doi:10.1016/s0168-1605(98)00109-3

Cited by 91 publications

(55 citation statements)

References 16 publications

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“…Our study demonstrates that the Bifidobacterium strains could be easily differentiated by RAPD fingerprinting, thus supporting the validity of the fast and accurate technique in studying diversity of lactic acid bacteria (5). This result is consistent with data from Vincent et al (16) which demonstrated that each 10-mer primer selected allowed differentiation of B. animalis, B. infantis, B. longum, B. adolescentis and B. breve. However, there is no report on the RAPD profile of B. pseudocatenulatum.…”

Section: Discussionsupporting

confidence: 90%

Differentiation of the Bifidobacterium pseudocatenulatum Isolates from Faeces of Infant by RAPD

Mustafa¹,

Ali²,

Saleh³

et al. 2001

Bioscience Microflora

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A total of 17 Bifidobacterium pseudocatenulatum strains isolated from infant faeces and 1 Bifidobacterium pseudocatenulatum type strain were compared for their clonal relatedness. Clonal relatedness was compared by randomly amplified polymorphic DNA analysis (RAPD) using three selected primers (GEN 1-60-03, GEN 1-60-06 and GEN 1-60-07). RAPD patterns with DNA fragments ranging from approximately 0.3 to 10.0 kb in size were observed. Examination of the DNA fingerprints using cluster analysis showed a significant genetic diversity among the strains tested.

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Section: Discussionsupporting

confidence: 90%

Differentiation of the Bifidobacterium pseudocatenulatum Isolates from Faeces of Infant by RAPD

Mustafa¹,

Ali²,

Saleh³

et al. 2001

Bioscience Microflora

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“…Regarding the bifidobacteria, Vincent et al (1998) noted that B. animalis and B. lactis exhibited high ho mology in their 16S rDNA sequencing. In this study, B. animalis ATCC 25527 and B. infantis ATCC 15697 were speciated as B. lactis and B. suis, respectively.…”

Section: Resultsmentioning

confidence: 99%

Species-Specific Identification of Commercial Probiotic Strains

Yeung¹,

Sanders²,

Kitts³

et al. 2002

Journal of Dairy Science

117

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Products containing probiotic bacteria are gaining popularity, increasing the importance of their accurate speciation. Unfortunately, studies have suggested that improper labeling of probiotic species is common in com mercial products. Species identification of a bank of com mercial probiotic strains was attempted using partial 16S rDNA sequencing, carbohydrate fermentation anal ysis, and cellular fatty acid methyl ester analysis. Re sults from partial 16S rDNA sequencing indicated dis crepancies between species designations for 26 out of 58 strains tested, including two ATCC Lactobacillus strains. When considering only the commercial strains obtained directly from the manufacturers, 14 of 29 strains carried species designations different from those obtained by partial 16S rDNA sequencing. Strains from six commercial products were species not listed on the label. The discrepancies mainly occurred in Lactobacil lus acidophilus and Lactobacillus casei groups. Carbohy drate fermentation analysis was not sensitive enough to identify species within the L. acidophilus group. Fatty acid methyl ester analysis was found to be variable and inaccurate and is not recommended to identify probi otic lactobacilli.

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“…B. longum subsp. longum CRC 002 genomic DNA was isolated from 1 ml overnight cultures in MRSCG2 medium as described previously (Vincent et al, 1998), with the following modification. The phenol/chloroform/isoamyl alcohol (25 : 24 : 1, by vol.)…”

Section: Methodsmentioning

confidence: 99%

Sugar source modulates exopolysaccharide biosynthesis in Bifidobacterium longum subsp. longum CRC 002

et al. 2010

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The effect of four sugars (glucose, galactose, lactose and fructose) on exopolysaccharide (EPS) production by Bifidobacterium longum subsp. longum CRC 002 was evaluated. More EPS was produced when CRC 002 was grown on lactose in the absence of pH control, with a production of 1080±120 mg EPS l "1 (P,0.01) after 24 h of incubation. For fructose, galactose and glucose, EPS production was similar, at 512±63, 564±165 and 616±93 mg EPS l "1 , respectively. The proposed repeating unit composition of the EPS is 2 galactose to 3 glucose. The effect of sugar and fermentation time on expression of genes involved in sugar nucleotide production (galK, galE1, galE2, galT1, galT2, galU, rmlA, rmlB1 and rmlCD) and the priming glycosyltransferase (wblE) was quantified using real-time reverse transcription PCR. A significantly higher transcription level of wblE (9.29-fold) and the genes involved in the Leloir pathway (galK, 4.10-fold; galT1, 2.78-fold; and galE2, 4.95-fold) during exponential growth was associated with enhanced EPS production on lactose compared to glucose. However, galU expression, linking glucose metabolism with the Leloir pathway, was not correlated with EPS production on different sugars. Genes coding for dTDP-rhamnose biosynthesis were also differentially expressed depending on sugar source and growth phase, although rhamnose was not present in the composition of the EPS. This precursor may be used in cell wall polysaccharide biosynthesis. These results contribute to understanding the changes in gene expression when different sugar substrates are catabolized by B. longum subsp. longum CRC 002.

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Characterization of bifidobacteria by random DNA amplification

Cited by 91 publications

References 16 publications

Differentiation of the Bifidobacterium pseudocatenulatum Isolates from Faeces of Infant by RAPD

Differentiation of the Bifidobacterium pseudocatenulatum Isolates from Faeces of Infant by RAPD

Species-Specific Identification of Commercial Probiotic Strains

Sugar source modulates exopolysaccharide biosynthesis in Bifidobacterium longum subsp. longum CRC 002

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