Characterization of Bacteria in Mixed Biofilm Communities Using Denaturing Gradient Gel Electrophoresis (<scp>DGGE</scp>)

Welsh, Allana; McLean, Robert

doi:10.1002/9780471729259.mc01e01s4

Cited by 7 publications

(4 citation statements)

References 20 publications

(28 reference statements)

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“…For example, Noguchi et al have identified 113 biofilmforming bacterial species on root surfaces outside the apical foramen and associated with refractory periapical periodontitis [12]. More often, the denaturing gradient gel electrophoresis (DGGE) method is employed to separate PCR-amplified community 16S rRNA on the basis of G+C content [13]. This method is widely used by different research groups to describe the microbial diversity and phylogenetic affiliation, and to identify individual species in multi-species biofilms [14][15][16][17].…”

Section: Methods For Study Of Multiple-species Biofilmsmentioning

confidence: 99%

Current understanding of multi‐species biofilms

et al. 2011

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Direct observation of a wide range of natural microorganisms has revealed the fact that the majority of microbes persist as surface-attached communities surrounded by matrix materials, called biofilms. Biofilms can be formed by a single bacterial strain. However, most natural biofilms are actually formed by multiple bacterial species. Conventional methods for bacterial cleaning, such as applications of antibiotics and/or disinfectants are often ineffective for biofilm populations due to their special physiology and physical matrix barrier. It has been estimated that billions of dollars are spent every year worldwide to deal with damage to equipment, contaminations of products, energy losses, and infections in human beings resulted from microbial biofilms. Microorganisms compete, cooperate, and communicate with each other in multi-species biofilms. Understanding the mechanisms of multi-species biofilm formation will facilitate the development of methods for combating bacterial biofilms in clinical, environmental, industrial, and agricultural areas. The most recent advances in the understanding of multi-species biofilms are summarized and discussed in the review.

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Section: Methods For Study Of Multiple-species Biofilmsmentioning

confidence: 99%

Current understanding of multi‐species biofilms

et al. 2011

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“…Finally, the results from PCR identification of the microorganisms can increase patient's motivation to maintain good oral hygiene. Unlike the bacterial cultivation method, PCR technique is carried out at the nucleic acid level and it doesn't depend on living bacteria presence [19,24]. This means simple collection and transport of the sample.…”

Section: Discussionmentioning

confidence: 99%

“…Molecular biology techniques have been developed and used for identification of the microbial organisms [14][15][16]. Polymerase chain reaction (PCR) is a simple technique which performs replication of a fragment from DNA and/or RNA and it has found its use in the detection of periodontopathic bacteria [17][18][19]. PCR doesn´t require living cells for identification of the microorganisms and offers significantly higher sensitivity than bacterial culture [20,21].…”

Section: Introductionmentioning

confidence: 99%

Identification of Periopathogenes from Dental Plaque in Periodontal Patients with PCR Technique and Their Association with Composite Interleukin-1 Genotype

Stojanovska

Todoroska²,

Поповска

et al. 2019

Prilozi

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Introduction: The present study aimed to assess the presence of main types of microorganisms involved in the aetiopathogenesis of chronic periodontitis with PCR technique and determinates the presence of composite IL-1 genotype and their associations with founded bacteria. Material and method: The examined group was consisted from 20 subjects with diagnosed chronic periodontitis and 20 healthy control without periodontitis. Clinical parameters like gingival index (GI), plaque index (PI), bleeding on probing (BOP), periodontal pocket depth (PPD) and clinical attachment lost (CAL) were determinates. Subgingival dental plaque was collected using a sterilized paper point. We used Parodontose Plus test, reverse hybridization kit, for the detection of periodontal marker bacteria, as well as for the detection of composite Interleukin -1 Genotype Results: The most present bacterial species detected from subgingival dental plaque was Treponema denticola and Porfiromonas gingivalis which was present in 65% of examined patients. In relation to the presence of positive genotype in patients, there was no significant difference between the test and control group for p> 0.05 (p = 1.00). For χ2=8,17 (p=0,06, p<0,05) there is an association between Prevotella intermedia, and composite genotype. Between positive genotype and analyzed bacterial species A. actinomycetem comitans for p> 0.05 (p = 1.00), P. gingivalis for p> 0.05 (p = 0.16), T. Forsythia for p> 0.05 (p = 0.20), T. Denticola for p> 0.05 (p = 0.64) no association was found. Conclusion. This investigations confirmed the strong association of these five examined periopathogenes with periodontitis.

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“…Biomass accrual is used as an indicator for overall biofilm growth. Structural changes are currently measured either by using traditional species identification with light microscopy or with nucleotide-based techniques (e.g., denaturing gradient gel electrophoresis (DGGE), automated ribosomal intergenic spacer analysis (ARISA), metagenomics) 10,11,12 . These methods provide information but are timeconsuming to perform, or require specific knowledge, or are still under development.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Aquatic Biofilms with Flow Cytometry

Sgier¹,

Merbt²,

Tlili³

et al. 2018

JoVE

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Biofilms are dynamic consortia of microorganism that play a key role in freshwater ecosystems. By changing their community structure, biofilms respond quickly to environmental changes and can be thus used as indicators of water quality. Currently, biofilm assessment is mostly based on integrative and functional endpoints, such as photosynthetic or respiratory activity, which do not provide information on the biofilm community structure. Flow cytometry and computational visualization offer an alternative, sensitive, and easy-to-use method for assessment of the community composition, particularly of the photoautotrophic part of freshwater biofilms. It requires only basic sample preparation, after which the entire sample is run through the flow cytometer. The single-cell optical and fluorescent information is used for computational visualization and biological interpretation. Its main advantages over other methods are the speed of analysis and the high-information-content nature. Flow cytometry provides information on several cellular and biofilm traits in a single measurement: particle size, density, pigment content, abiotic content in the biofilm, and coarse taxonomic information. However, it does not provide information on biofilm composition on the species level. We see high potential in the use of the method for environmental monitoring of aquatic ecosystems and as an initial biofilm evaluation step that informs downstream detailed investigations by complementary and more detailed methods.

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Characterization of Bacteria in Mixed Biofilm Communities Using Denaturing Gradient Gel Electrophoresis (DGGE)

Cited by 7 publications

References 20 publications

Current understanding of multi‐species biofilms

Current understanding of multi‐species biofilms

Identification of Periopathogenes from Dental Plaque in Periodontal Patients with PCR Technique and Their Association with Composite Interleukin-1 Genotype

Characterization of Aquatic Biofilms with Flow Cytometry

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