1990
DOI: 10.1007/978-1-4684-5823-7_55
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Characterization of Attenuated Mutants of MHV3 : Importance of the E2 Protein in Organ Tropism and Infection of Isolated Liver Cells

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Cited by 6 publications
(7 citation statements)
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“…We have previously observed that both MHV-A59 (19) and a very weakly hepatotropic strain (C12) (unpublished results) replicate to very similar low titers, suggesting that the hepatocytes are not a major site of replication for MHV-A59. By comparing the highly hepatotropic MHV-3 with MHV-4, it has been also demonstrated that replication in hepatic endothelial cells but not hepatocytes correlated with hepatotropism (22,34,37,38). Our data are consistent with the notion that all three strains may infect the vessel cells but that replication and/or spread from these cells into hepatocytes might be much less efficient with MHV-4.…”
supporting
confidence: 87%
“…We have previously observed that both MHV-A59 (19) and a very weakly hepatotropic strain (C12) (unpublished results) replicate to very similar low titers, suggesting that the hepatocytes are not a major site of replication for MHV-A59. By comparing the highly hepatotropic MHV-3 with MHV-4, it has been also demonstrated that replication in hepatic endothelial cells but not hepatocytes correlated with hepatotropism (22,34,37,38). Our data are consistent with the notion that all three strains may infect the vessel cells but that replication and/or spread from these cells into hepatocytes might be much less efficient with MHV-4.…”
supporting
confidence: 87%
“…Our results are consistent with the notion that our MV3 escape mutants display at least one single mutation in a determinant of the S glycoprotein. These mutations could be responsible for impairment in recognition of a cellular receptor by the virus in hepatic cells (Martin et al, 1990), thus hindering its multiplication in the liver and delaying the evolution of the disease.…”
Section: In This Work We Compared the In Vivo And In Vitro Virulencementioning
confidence: 99%
“…We prepared monoclonal antibodies (mAb) for the S glycoprotein and used them to select attenuated MHV3 escape mutants for further investigations into the viral pathogenicity and hepatotropism of MHV3, which produces an acute fulminating hepatitis with extensive necrosis of the liver in susceptible BALB/c mice (Lucchiari et al, 1991;Martin etal., 1988;1990).…”
mentioning
confidence: 99%
“…The mAb were chosen to select escape mutants retaining partial pathogenic property. These mAb were able to neutralize MHV3 and to slightly inhibit cellular fusion, but did not protect the sensitive mice against MHV3 infection (Martin et al, 1990). All viruses were cultured in L2 cells.…”
Section: Virusesmentioning
confidence: 99%
“…In contrast, virus-mediated fusion activity is located at a site different from the neutralization epitopes, as shown by the binding sites of several mAb (Collins et al, 1982 ;Routledge et al, 1991). MAb used for the selection of attenuated MHV3 mutants were able to neutralize L2-MHV3 at high dilutions and to slightly inhibit cellular fusion, but did not protect sensitive mice against pathogenic L2-MHV3 infection (Martin et al, 1990), suggesting that these viruses are modified at a site included in a neutralization epitope and located near the fusion site, probably on the S2 portion of the viral glycoprotein (Daniel et al, 1993).…”
Section: Inflammatorymentioning
confidence: 99%