Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides

Saleh, Shireen A.A.; Wahab, Walaa A. Abdel; El-Dein, Asmaa Negm; Abdelwahab, Wesam; Ahmed, Amal Abdel Majid; Helmy, Wafaa A.; Mostafa, Faten A.

doi:10.1016/j.ijbiomac.2020.10.225

Cited by 18 publications

(6 citation statements)

References 42 publications

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“…Generally, oligo-saccharides usually possess a great deal of prebiotic activity. Saleh et al 60 reported a variable promotion effect (18.75–25%) of xylooligosaccharides produced by Aspergillus niger MK981235 towards L. plantarum .…”

Section: Resultsmentioning

confidence: 99%

Coculture of bacterial levans and evaluation of its anti-cancer activity against hepatocellular carcinoma cell lines

Wahab,

Shafey,

Mahrous

et al. 2024

Sci Rep

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This research represents a novel study to assess how coculture affects levan yield, structure, bioactivities, and molecular weight. Among the 16 honey isolates, four bacterial strains recorded the highest levan yield. The Plackett–Burman design showed that the coculture (M) of isolates G2 and K2 had the maximum levan yield (52 g/L) and the effective factors were sucrose, incubation time, and sugarcane bagasse. The CCD showed that the most proper concentrations for maximum levan yield (81 g/L): were 130 g/L of sucrose and 6 g/f of sugarcane bagasse. Levan’s backbone was characterized, and the molecular weight was determined. G2 and K2 isolates were identified based on 16 sRNA as Bacillus megaterium strain YM1C10 and Rhizobium sp. G6-1. M levan had promising antioxidant activity (99.66%), slowed the migration activity to a great extent, and recorded 70.70% inhibition against the hepatoblastoma cell line (HepG2) at 1000 µg/mL. Gene expression analysis in liver cancer cell lines (HePG2) revealed that M levan decreased the expression of CCL20), 2GRB2, and CCR6) genes and was superior to Doxo. While increasing the expression of the IL4R and IL-10 genes. The DNA damage values were significantly increased (P < 0.01) in treated liver cancer cell lines with levan M and Doxo. The results referred to the importance of each of the hydroxyl and carboxyl groups and the molecular weight in levans bioactivities.

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Section: Resultsmentioning

confidence: 99%

Coculture of bacterial levans and evaluation of its anti-cancer activity against hepatocellular carcinoma cell lines

Wahab,

Shafey,

Mahrous

et al. 2024

Sci Rep

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“…Among all xylanase-producing fungi, since Aspergillus sp. is the most common commercial source of xylanase, those with high productivity yields are particularly interesting [6,42,43]. Internal transcribed spacer (ITS)-based identification has been considered effective in taxonomy identification because it is accurate and quick and only requires a small amount of sample.…”

Section: Discussionmentioning

confidence: 99%

Safe production of Aspergillus terreus xylanase from Ricinus communis: gene identification, molecular docking, characterization, production of xylooligosaccharides, and its biological activities

Nour

El-Sayed

Taie

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background The production of industrial enzymes such as xylanase using sufficient cost-effective substrates from potent microorganisms is considered economically feasible. Studies have reported castor cake (Ricinus communis) as the most potent and inexpensive alternative carbon source for production of xylanase C by using Aspergillus terreus (A. terreus). Results A. terreus strain RGS Eg-NRC, a local isolate from agro-wastes, was first identified by sequencing the internal transcribed spacer region of a nuclear DNA encoding gene cluster deposited in GenBank (accession number MW282328). Before optimization of xylanase production, A. terreus produced 20.23 U/g of xylanase after 7 days using castor cake as a substrate in a solid-state fermentation (SSF) system that was employed to achieve ricin detoxification and stimulate xylanase production. Physicochemical parameters for the production of xylanase were optimized by using a one-variable-at-a-time approach and two statistical methods (two-level Plackett–Burman design and central composite design, CCD). The maximum xylanase yield after optimization was increased by 12.1-fold (245 U/g). A 60–70% saturation of ammonium sulfate resulted in partially purified xylanase with a specific activity of 3.9 IU/mg protein. At 60 °C and pH 6, the partially purified xylanase had the highest activity, and the activation energy (Ea) was 23.919 kJmol. Subsequently, antioxidant capacity and cytotoxicity tests in normal Ehrlich ascites carcinoma human cells demonstrated xylooligosaccharides produced by the xylanase degradation of xylan as a potent antioxidant and moderate antitumor agent. Further investigations with sodium dodecyl sulfate polyacrylamide gel electrophoresis then determined the molecular weight of partially purified xylanase C to be 36 kDa. Based on the conserved regions, observations revealed that xylanase C belonged to the glycosyl hydrolase family 10. Next, the xylanase-encoding gene (xynC), which has an open reading frame of 981 bp and encodes a protein with 326 amino acids, was isolated, sequenced, and submitted to the NCBI GenBank database (accession number LC595779.1). Molecular docking analysis finally revealed that Glu156, Glu262, and Lys75 residues were involved in the substrate-binding and protein-ligand interaction site of modeled xylanase, with a binding affinity of −8.7 kcal. mol−1. Conclusion The high production of safe and efficient xylanase could be achieved using economical materials such as Ricinus communis.

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“…XOS generated after the hydrolysis of xylan are short-chain XOS and consist of xylobiose, xylotriose, and xylotetraose. XOS can be produced by the action of xylan-degrading enzymes such as endo-β-1,4-xylanase (EC 3.2.1.8) and xylan 1, 4-β-xylosidase (EC 3.2.1.37), with the help of other side chain-degrading enzymes, like α-glucuronidase (EC 3.2.1.139), acetyl xylan esterase (EC 3.1.1.72), α-L-feruloyl esterase (EC 3.1.1.73), and arabinofuranosidase (EC 3.2.1.55) ( 32 ). The sources and structural details of xylan-derived MOS are presented in Figure 1 .…”

Section: Types Of Hdosmentioning

confidence: 99%

Hemicellulose-Derived Oligosaccharides: Emerging Prebiotics in Disease Alleviation

Jana

Kango²,

Pletschke³

2021

Front. Nutr.

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The gut microbiota in the human body is an important component that plays a pivotal role in the ability of the host to prevent diseases and recover from these diseases. If the human microbiome changes for any reason, it affects the overall functioning of the host. Healthy and vigorous gut microbiota require dietary fiber supplementation. Recently, oligosaccharides have been found to play a significant role in the modulation of microbiota. Several such oligosaccharides, i.e., xylooligosaccharides (XOS), mannooligosaccharides (MOS), and arabino-xylooligosaccharides (AXOS), are derived from hemicellulosic macromolecules such as xylan, mannan, and arabino-xylan, respectively. These oligosaccharides serve as substrates for the probiotic production of health-promoting substances (short-chain fatty acids, branched chain amino acids etc.), which confer a variety of health benefits, including the prevention of some dreaded diseases. Among hemicellulose-derived oligosaccharides (HDOs), XOS have been largely explored, whereas, studies on MOS and AXOS are currently underway. HDOs, upon ingestion, help reduce morbidities by lowering populations of harmful or pathogenic bacteria. The ATP-binding cassette (ABC) transporters are mainly utilized for the uptake of oligosaccharides in probiotics. Butyrate generated by the selective fermentation of oligosaccharides, along with other short-chain fatty acids, reduces gut inflammation. Overall, oligosaccharides derived from hemicelluloses show a similar potential as conventional prebiotics and can be supplemented as functional foods. This review summarizes the role of HDOs in the alleviation of autoimmune diseases (inflammatory bowel disease, Crohn's disease), diabetes, urinary tract infection, cardiovascular diseases, and antimicrobial resistance (AMR) through the modulation of the gut microbiota. The mechanism of oligosaccharide utilization and disease mitigation is also explained.

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Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides

Cited by 18 publications

References 42 publications

Coculture of bacterial levans and evaluation of its anti-cancer activity against hepatocellular carcinoma cell lines

Coculture of bacterial levans and evaluation of its anti-cancer activity against hepatocellular carcinoma cell lines

Safe production of Aspergillus terreus xylanase from Ricinus communis: gene identification, molecular docking, characterization, production of xylooligosaccharides, and its biological activities

Hemicellulose-Derived Oligosaccharides: Emerging Prebiotics in Disease Alleviation

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