Characterization of Ascochyta as Pathological Species of Pea (Pisum sativum L.) at the North-West of Algeria

Tadja, Abdelkader; Benkada, Mokhtar Youcef; Rickauer, Martina; Bendahmane, Boubekeur Seddik; Benkhelifa, Mohammed

doi:10.3923/ja.2009.100.106

Cited by 6 publications

(5 citation statements)

References 9 publications

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“…In this study, we collected 65 isolates from 5 sites in this area and identified them based on ITS and G3PDH sequence alignments. Previous studies (Fatehi et al, 2003 ; Peever et al, 2007 ; Davidson et al, 2009 ; Tadja et al, 2009 ) have indicated that the ITS sequences can be an effective tool to separate A. pinodes and P. pinodella from A. pisi and P. koolunga , whereas they did not allow differentiation between A. pinodes and P. pinodella . Consistent with this finding, the ITS sequences of all 65 isolates showed 100% identity to both A. pinodes and P. pinodella (Figure 1C ).…”

Section: Discussionmentioning

confidence: 97%

Studies on the Control of Ascochyta Blight in Field Peas (Pisum sativum L.) Caused by Ascochyta pinodes in Zhejiang Province, China

Liu

Yao

et al. 2016

Front. Microbiol.

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Ascochyta blight, an infection caused by a complex of Ascochyta pinodes, Ascochyta pinodella, Ascochyta pisi, and/or Phoma koolunga, is a destructive disease in many field peas (Pisum sativum L.)-growing regions, and it causes significant losses in grain yield. To understand the composition of fungi associated with this disease in Zhejiang Province, China, a total of 65 single-pycnidiospore fungal isolates were obtained from diseased pea samples collected from 5 locations in this region. These isolates were identified as Ascochyta pinodes by molecular techniques and their morphological and physiological characteristics. The mycelia of ZJ-1 could penetrate pea leaves across the stomas, and formed specific penetration structures and directly pierced leaves. The resistance level of 23 available pea cultivars was tested against their representative isolate A. pinodes ZJ-1 using the excised leaf-assay technique. The ZJ-1 mycelia could penetrate the leaves of all tested cultivars, and they developed typical symptoms, which suggested that all tested cultivars were susceptible to the fungus. Chemical fungicides and biological control agents were screened for management of this disease, and their efficacies were further determined. Most of the tested fungicides (11 out of 14) showed high activity toward ZJ-1 with EC 50 < 5 µg/mL. Moreover, fungicides, including tebuconazole, boscalid, iprodione, carbendazim, and fludioxonil, displayed more than 80% disease control efficacy under the recorded conditions. Three biocontrol strains of Bacillus sp. and one of Pantoea agglomerans were isolated from pea-related niches and significantly reduced the severity of disease under greenhouse and field conditions. To our knowledge, this is the first study on ascochyta blight in field peas, and results presented here will be useful for controlling the disease in this area.

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Section: Discussionmentioning

confidence: 97%

Studies on the Control of Ascochyta Blight in Field Peas (Pisum sativum L.) Caused by Ascochyta pinodes in Zhejiang Province, China

Liu

Yao

et al. 2016

Front. Microbiol.

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“…Internal transcribed spacer (ITS) sequences can be used to discriminate M. pinodes and P. medicaginis var. pinodella from A. pisi and P. koolunga [2, [31][32][33]. Recently, ITS sequence analysis was used to demonstrate that A. pisi and P. koolunga are likely not present in central Alberta [11].…”

Section: Cultural Characterizationmentioning

confidence: 99%

Morphological characterization of fungi associated with the ascochyta blight complex and pathogenic variability of Mycosphaerella pinodes on field pea crops in central Alberta

et al. 2015

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Field pea crops in central Alberta were surveyed for ascochyta blight from 2011 to 2012 and fungal isolates were recovered from foliar lesions on selected plants. Cultural and microscopic characterization of the 275 isolates obtained revealed that 272 were of Mycosphaerella pinodes and three were of Phoma medicaginis var. pinodella. Ascochyta pisi or Phoma koolunga were not identified. Isolates of M. pinodes were divided into two groups, GI and GII, based on visual assessment of culture characteristics. GI isolates (light to dark, mostly gray colony color; pycnidial distribution radial and concentric; conidia 10.5-14.5 × 4.2-6.2 μm most with one septum, occasionally two, constricted at the septum; spore mass light buff to flesh color) were predominant (83%), while GII isolates (dark to gray colony color; pycnidia abundant; conidia 8-16 × 3.5-6.2 μm most with 1 septum, constricted at the septum; spore mass light buff to flesh color) were less common (17%). The cultures of GII isolates were similar to recent descriptions of A. pisi, but they differed in spore color. In a host differential study, 13 pathotypes of M. pinodes were identified from 110 single-spore isolates. Pathotype I was predominant (88 isolates) and virulent on all nine differential genotypes. The other pathotypes (pathotypes II-XIII) were rare (1-6 isolates of each).Comparison of the present results with earlier studies suggests that pathotype I has been prevalent for many years, and that its aggressiveness on the host differentials has increased over time. Emphasis should be placed on breeding for resistance to M. pinodes in field pea cultivars intended for deployment in central Alberta.

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“…The detected seed-borne fungi were identified according to Common Wealth Mycological Institute Description Sheets, Danish Government Institute of Seed Pathology Publication, and Research work of Tadja et al (2009). Identification was kindly confirmed by Taxonomy Dep.…”

Section: Detection and Isolation Of Some Legume Seed-borne Pathogens mentioning

confidence: 99%

MOLECULAR DETECTION AND CONTROLLING OF SEED-BORNE Colletotrichum spp. IN COMMON BEAN AND SOYBEAN

Sewedy

Atia

Zayed

et al. 2019

Zagazig Journal of Agricultural Research

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Seed-borne fungi cause several diseases of common bean (Phaseolus vulgaris L.) and soybean (Glycine max L.). The survey of seed-borne fungi of common bean and soybean was carried out on three Egyptian Governorates (Behera, Dakahlia, and Ismailia) in Egypt. Nineteen fungal species comprising therteen genera were isolated from the collected common bean and soybean seed samples, using standard blotter i,e.

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Characterization of Ascochyta as Pathological Species of Pea (Pisum sativum L.) at the North-West of Algeria

Cited by 6 publications

References 9 publications

Studies on the Control of Ascochyta Blight in Field Peas (Pisum sativum L.) Caused by Ascochyta pinodes in Zhejiang Province, China

Studies on the Control of Ascochyta Blight in Field Peas (Pisum sativum L.) Caused by Ascochyta pinodes in Zhejiang Province, China

Morphological characterization of fungi associated with the ascochyta blight complex and pathogenic variability of Mycosphaerella pinodes on field pea crops in central Alberta

MOLECULAR DETECTION AND CONTROLLING OF SEED-BORNE Colletotrichum spp. IN COMMON BEAN AND SOYBEAN

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