Characterization of antibody response in neuroinvasive infection caused by Toscana virus

Pierro, Anna; Ficarelli, S.; Ayhan, Nazlı; Morini, Silvia; Raumer, Luigi; Bartoletti, Michele; Mastroianni, Antonio; Prati, Francesca; Schivazappa, Simona; Cenni, P.; Vocale, Caterina; Rossini, Giada; Gaibani, Paolo; Sambri, Vittorio; Landini, M.P.; Lewis, Russell E.; Charrel, Rémi N.; Varani, Stefania

doi:10.1016/j.cmi.2017.03.017

Cited by 20 publications

(24 citation statements)

References 27 publications

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“…In this study, we observed that anti-TOSV positive BDs resided mainly in municipalities of the Bologna province, while no active TOSV circulation was observed in the Ferrara province; these findings match with estimated abundance of sand flies in these two provinces of Northeastern Italy. Between 2010 and 2013, 143 cases of neuroinvasive infection caused by TOSV were identified in the Emilia-Romagna region, Northeastern Italy; the highest number of TOSV cases was recorded in the Bologna province and the lowest number in the Ferrara province [17,18]. In line with the distribution of symptomatic TOSV cases, our serosurvey identified a high circulation of TOSV in the Bologna province, with a seroprevalence of 6.8%, while no TOSV circulation was observed in the Ferrara province.…”

Section: Discussionsupporting

confidence: 73%

“…Each serum sample was first evaluated for the presence of specific TOSV-IgG using an in-house ELISA, as described [18]. Briefly, the viral antigen consisted of purified and inactivated virus, obtained from TOSV strain MRS2010-4319501 isolated in Marseille.…”

Section: Detection Of Anti-tosv Igg By Elisamentioning

confidence: 99%

“…Microorganisms 2020, 8, 145 4 of 10 2.2.3. Detection of Anti-TOSV and Anti-SFSV Neutralizing Antibody MN was performed as described [18]. Briefly, 100 TCID50 (50% tissue culture infective dose) of TOSV (strain MRS2010-4319501) or SFSV (strain UVE/SFSV/1943/IT/Sabin, Ref#001v-EVA77 accessible at https://www.european-virus-archive.com/virus/sandfly-fever-sicilianvirus-strain-uvesfsv1943itsabin) were added to wells of a 96-well plate containing patient serum and incubated at 37 • C for 1 h. Subsequently, a 100 µL suspension of Vero cells containing approximately 2 × 10 5 cells/mL was added to each well and incubated for 5 days.…”

Section: Iif For Anti-tosv Igg Detectionmentioning

confidence: 99%

“…Since 1999, autochthonous human cases of TOSV meningitis have been reported in the Emilia-Romagna region (Northeastern Italy), indicating local circulation of the virus [16]. In 2010, a surveillance plan to monitor human arboviral cases was adopted by the Emilia-Romagna region, leading to the detection of 61 cases of TOSV meningitis or meningoencephalitis in 2010-2012 [17] and 82 cases in summer-autumn 2013 [18]. Two sand fly species are present in the Emilia-Romagna region, i.e., Ph.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Specific Antibodies against Toscana Virus among Blood Donors in Northeastern Italy and Correlation with Sand Fly Abundance in 2014

et al. 2020

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Toscana virus (TOSV) is a Phlebovirus transmitted by phlebotomine sand flies and is an important etiological agent of summer meningitis in the Mediterranean basin. Since TOSV infection is often asymptomatic, we evaluated the seroprevalence in blood donors (BDs) in the Bologna and Ferrara provinces (Northeastern Italy)—the areas with the highest and lowest numbers of TOSV neuroinvasive cases in the region, respectively. A total of 1208 serum samples from BDs were collected in April–June 2014 and evaluated for the presence of specific TOSV-IgG by ELISA. The IgG-reactive samples were confirmed by indirect immunofluorescence assay (IIF) and by microneutralization test (MN). Serum samples were defined as positive for anti-TOSV IgG when reactive by ELISA and by at least one second-level test; TOSV seroprevalence was 6.8% in the Bologna province, while no circulation of TOSV was detected in the Ferrara province. Sand fly abundance in 2014 was also estimated by a geographic information system using a generalized linear model applied to a series of explanatory variables. TOSV seroprevalence rate was strongly associated with the sand fly abundance index in each municipality, pointing out the strong association between sand fly abundance and human exposure to TOSV.

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Section: Discussionsupporting

confidence: 73%

Section: Detection Of Anti-tosv Igg By Elisamentioning

confidence: 99%

Section: Iif For Anti-tosv Igg Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Specific Antibodies against Toscana Virus among Blood Donors in Northeastern Italy and Correlation with Sand Fly Abundance in 2014

et al. 2020

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“…Seroepidemiological studies showed the presence of neutralizing antibodies against Toscana virus in several Mediterranean countries, however, the rates vary depending on the region Mediterranean basin considered as endemic region of Toscana virus [45,71,74,78,[83][84][85][86][87][88].…”

Section: Toscana Virusmentioning

confidence: 99%

Sandfly-Borne Viruses of Demonstrated/Relevant Medical Importance

Ayhan¹,

Charrel²

2019

Vectors and Vector-Borne Zoonotic Diseases

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Sandflies show distribution in a vast geographical area from Europe to Asia, Africa, Australia, and Central and South America where they can transmit a large number of viruses. Between these viruses, the most important are grouped into the Phlebovirus genus (family Phenuiviridae). Among them, several sandfly-borne phleboviruses cause self-limiting febrile disease (sandfly fever) or central and peripheral nervous system infections. Data concerning the geographic distribution of these phleboviruses has drastically increased during the last decade in both the new and the old worlds. The current situation depicts a high viral diversity with taxonomic groups containing human pathogenic and non-pathogenic viruses. This merits to provide insight to address the question of medical and veterinary public health impact of all these viruses, which are poorly studied. To do so, integrated and translational approaches must use ecological, epidemiological, serological and direct clinical evidence. Beside, other viruses transmitted by sandflies and belonging to Rhabdoviridae and Reoviridae families can also be of veterinary and public health importance. The chapter aims to provide a comprehensive view of the sandfly-borne viral pathogens of the public health impact on humans and other vertebrates in the old and new worlds.

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Neutralizing based seroprevalence study of Toscana virus in livestock from Algeria

Sellali,

Lafri,

Ayhan

et al. 2023

Comparative Immunology, Microbiology and Infectious Diseases

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Characterization of antibody response in neuroinvasive infection caused by Toscana virus

Cited by 20 publications

References 27 publications

Detection of Specific Antibodies against Toscana Virus among Blood Donors in Northeastern Italy and Correlation with Sand Fly Abundance in 2014

Detection of Specific Antibodies against Toscana Virus among Blood Donors in Northeastern Italy and Correlation with Sand Fly Abundance in 2014

Sandfly-Borne Viruses of Demonstrated/Relevant Medical Importance

Neutralizing based seroprevalence study of Toscana virus in livestock from Algeria

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