Characterization of Antibody Immobilization on Chitosan/Gelatin-Modified Electrode and Its Application to Bacillus cereus Detection in Cereal-Based Food

Yashini, M; Auddy, Ishita; Shanmugasundaram, S.; Vidyalakshmi, R.; Sunil, C. K.

doi:10.1007/s12161-022-02299-y

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…The sensor response reduces due to matrix interferences and is highly specific for the pathogen under specific detection range (Chowdhury et al , 2012). Yashini et al (2022) developed the electrochemical immunosensor for label-free detection of Bacillus cereus. They used a modified chitosan/gelatin electrode and exhibited a LOD and LOQ of 10 and 103 CFU/mL, respectively, and the detection time is less than 50 min.…”

Section: Classification Of Biosensormentioning

confidence: 99%

Application of biosensors against food-borne pathogens

Singh,

Sharanagat

2023

NFS

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Purpose Nature and occurrence of food-borne pathogens in raw and processed food products evolved greatly in the past few years due to new modes of transmission and resistance build-up against sundry micro-/macro-environmental conditions. Assurance of food health and safety thus gained immense importance, for which bio-sensing technology proved very promising in the detection and quantification of food-borne pathogens. Considering the importance, different studies have been performed, and different biosensors have been developed. This study aims to summarize the different biosensors used for the deduction of food-borne pathogens. Design/methodology/approach The present review highlights different biosensors developed apropos to food matrices, factors governing their selection, their potential and applicability. The paper discusses some related key challenges and constraints and also focuses on the needs and future research prospects in this field. Findings The shift in consumers’ and industries’ perceptions directed the further approach to achieve portable, user and environmental friendly biosensing techniques. Despite of these developments, it was still observed that the comparison among the different biosensors and their categories proved tedious on a single platform; since the food matrices tested, pathogen detected or diagnosed, time of detection, etc., varied greatly and very few products have been commercially launched. Conclusively, a challenge lies in front of food scientists and researchers to maintain pace and develop techniques for efficiently catering to the needs of the food industry. Research limitations/implications Biosensors deduction limit varied with the food matrix, type of organism, material of biosensors’ surface, etc. The food matrix itself consists of complex substances, and various types of food are available in nature. Considering the diversity of food there is a need to develop a universal biosensor that can be used for all the food matrices for a pathogen. Further research is needed to develop a pathogen-specific biosensor that can be used for all the food products that may have accuracy to eliminate the traditional method of deduction. Originality/value The present paper summarized and categorized the different types of biosensors developed for food-borne pathogens. Graphical abstract

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Section: Classification Of Biosensormentioning

confidence: 99%

Application of biosensors against food-borne pathogens

Singh,

Sharanagat

2023

NFS

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“…Biosensors with gelatin as matrix for immobilized biorecognition materials including several additional works [ 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ] are summarized in Table 1 .…”

Section: Gelatin As a Matrix For Immobilized Biorecognition Materialsmentioning

confidence: 99%

Applications of Gelatin in Biosensors: Recent Trends and Progress

Guan

Huang

2022

Biosensors

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Gelatin is a natural protein from animal tissue with excellent biocompatibility, biodegradability, biosafety, low cost, and sol–gel property. By taking advantage of these properties, gelatin is considered to be an ideal component for the fabrication of biosensors. In recent years, biosensors with gelatin have been widely used for detecting various analytes, such as glucose, hydrogen peroxide, urea, amino acids, and pesticides, in the fields of medical diagnosis, food testing, and environmental monitoring. This perspective is an overview of the most recent trends and progress in the development of gelatin-based biosensors, which are classified by the function of gelatin as a matrix for immobilized biorecognition materials or as a biorecognition material for detecting target analytes.

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“…Moreover, the surface of the electrodes is a key point affecting the response of electrochemical immunosensors [23]. Conducting polymers [24], natural polymers [25], inorganic materials [26], and nanostructures [27] are used for surface modification to prepare immunosensors. Among them, electrospun nanofibers have attracted considerable attention because of their large surface area, controllable film thickness, and ease of forming functional groups on their surface.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Nanofibers including Organic/Inorganic Nanohybrids: Polystyrene- and Clay-Based Architectures in Immunosensor Preparation for Serum Amyloid A

Evren,

Er,

Yalcinkaya

et al. 2023

Biosensors

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Diagnostic techniques based on biomolecules have application potential that can be realized in many fields, such as disease diagnosis, bioprocess imaging, food/beverage industries, and environmental pollutant imaging. Successful surface immobilization of biomolecules is critical to increasing the stabilization, sensitivity, and selectivity of biomolecules used in bioassay systems. Nanofibers are good candidates for the immobilization of biomolecules owing to many advantages such as morphology and pore size. In this study, montmorillonite (MMT) clay is modified with poly(amidoamine) (PAMAM) generation 3 (PAMAMG3) and added to polystyrene (PS) solutions, following which PS/MMT-PAMAMG3 nanofibers are obtained using the electrospinning method. The nanofibers are obtained by testing PS% (wt%) and MMT-PAMAMG3% (wt%) ratios and characterized with scanning electron microscopy. Antiserum amyloid A antibody (Anti-SAA) is then conjugated to the nanofibers on the electrode surface via covalent bonds using a zero-length cross linker. Finally, the obtained selective surface is used for electrochemical determination of serum amyloid A (SAA) levels. The linear range of PS/MMT-PAMAM/Anti-SAA is between 1 and 200 ng/mL SAA, and the detection limit is 0.57 ng/mL SAA. The applicability of PS/MMT-PAMAMG3/Anti-SAA is investigated by taking measurements in synthetic saliva and serum both containing SAA.

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Characterization of Antibody Immobilization on Chitosan/Gelatin-Modified Electrode and Its Application to Bacillus cereus Detection in Cereal-Based Food

Cited by 7 publications

References 40 publications

Application of biosensors against food-borne pathogens

Application of biosensors against food-borne pathogens

Applications of Gelatin in Biosensors: Recent Trends and Progress

Electrospun Nanofibers including Organic/Inorganic Nanohybrids: Polystyrene- and Clay-Based Architectures in Immunosensor Preparation for Serum Amyloid A

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