Characterization of Antibodies in Human Urine*

Hanson, Lars Å.; Tan, Eng M.

doi:10.1172/jci105183

Cited by 52 publications

(15 citation statements)

References 39 publications

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“…In addition to these intact proteins, various immunoglobulin fragments have been reported [3,4,6,11,12,28,30]. Intact IgM has not been detected in normal urine although IgM fragments have recently been demonstrated in human urine following strenuous exercise [23].…”

Section: Resultsmentioning

confidence: 99%

Studies on the Levels of Immunoglobulins in Normal Human Urine with Particular Reference to IgE

Turner

Johansson

Barratt

et al. 1970

Int Arch Allergy Immunol

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Section: Resultsmentioning

confidence: 99%

Studies on the Levels of Immunoglobulins in Normal Human Urine with Particular Reference to IgE

Turner

Johansson

Barratt

et al. 1970

Int Arch Allergy Immunol

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“…It was probable that eluates of cortex homogenates included 'y-globulin derived from one or more of these loci, depending on the kidney specimens under study. Tubular protein casts might be considered a source of the eluted reactive globulin, inasmuch as y-globulinuria is a common finding in lupus nephritis (13), and antinuclear activity has been demonstrated in concentrated urine specimens (13,14). However, acid washing of kidney specimen sections performed in a manner similar to the elution procedure was found not to diminish the extent of fluorescent staining of yG-globulin in tubular casts, whereas in the same sections staining was markedly diminished or abolished in glomeruli.…”

Section: Discussionmentioning

confidence: 95%

Immunopathologic Studies of Systemic Lupus Erythematosus. II. Antinuclear Reaction of γ-Globulin Eluted from Homogenates and Isolated Glomeruli of Kidneys from Patients with Lupus Nephritis *

Krishnan¹,

Kaplan²

1967

J. Clin. Invest.

202

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Summary. The yG-globulin eluted at acid pH from kidney cortex homogenates and isolated glomeruli of five of six patients with lupus nephritis was found to exhibit antinuclear activity, which was not dependent on presence of fresh human serum. Specificity, as demonstrated by absorption of antinuclear activity, was related to nucleoprotein in three glomerular acid eluates and to DNA in two acid eluates as well as in a deoxyribonuclease digest of disrupted glomeruli in one patient. Antinuclear activity was not found in acid eluates of kidneys from two patients with chronic liver disease and chronic discoid lupus, respectively, and one with lupus nephritis. These patients had a low titer of serum antinuclear factor and lesser amounts of kidney bound immunoglobulins. The presence of antinuclear activity in eluates of kidneys appeared to correlate with the amount of glomerular bound immunoglobulin and the level of antinuclear antibodies in serum. These findings suggest that in lupus nephritis, part of the glomerular bound immunoglobulin is derived from serum antinuclear factors possibly deposited as immune complexes. IntroductionThe definition of immunoglobulin deposits in lupus kidneys has been approached by techniques applicable to dissociation of antigen-antibody complexes. Sections of lupus nephritis kidneys washed at acid pH (3.0 to 3.2) were found to lose considerable staining for y-globulin in glomerular and vas-

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“…Previous investigators have demonstrated antibody activity in urine associated with low molecular weight gamma globulins, IgG and IgA (34)(35)(36). Vosti and Remington demonstrated that the anti-O antibody in the urine of previously infected patients was exclusively IgG (37).…”

Section: Local Immune Response In Experimental Pyelonephritismentioning

confidence: 99%