Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF

Salih, Enass Y. A.; Julkunen‐Tiitto, Riitta; Kanninen, Markku; Sipi, Marketta; Luukkanen, Olavi; Vuorela, Heikki; Fyhrquist, Pia

doi:10.1055/s-0035-1565454

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these compound groups, we and other researchers have reported on the occurrence of a number of ellagitannins, ellagic acid and its derivatives, flavellagic acid, gallic acid and its derivatives, gallotannins, epicatechin-3-O-gallate, and epicatechin [18,[26][27][28][29][30] (Figure 3). In addition, a number of flavonoids have been reported to occur in A. leiocarpa (Figure 3) [16,18,26,[31][32][33][34]. [18,26,27].…”

Section: Introductionmentioning

confidence: 99%

Potential Anti-Tuberculosis Activity of the Extracts and Their Active Components of Anogeissus leiocarpa (DC.) Guill. and Perr. with Special Emphasis on Polyphenols

et al. 2020

View full text Add to dashboard Cite

In Sudanese traditional medicine, decoctions of the stem bark of Anogeissus leiocarpa are used for the treatment of tuberculosis (TB). However, this plant has not been investigated before for its antimycobacterial effects. Our screening results show, for the first time, that many extracts of various parts of A. leiocarpa exhibit growth inhibitory activity against Mycobacterium smegmatis. Minimum inhibitory concentration (MIC) values ranged between 625 and 5000 µg/mL, with an ethyl acetate extract of the root showing the lowest MIC value. The good antimycobacterial effects of the root part could be due to its high concentration of ellagic acid derivatives, ellagitannins, and flavonoids. Thin layer chromatography (TLC) fractionation resulted in some fractions with better activity than the starting point crude methanol extract (MIC 2500 µg/mL). Those fractions with the lowest MIC values contained a high number of antioxidant compounds. Fractions 3 and 4 (MIC 1500 and 1000 µg/mL, respectively) contained high concentrations of di-methyl ellagic acid ([M-H]− 329.0318). Fraction 6 (MIC 2000 µg/mL) contained a lower concentration of di-methyl ellagic acid and was not as growth inhibitory as fractions 3 and 4. Moreover, in fraction 3, an acetylated ellagic acid derivative ([M-H]− 343.0477) and di-methyl-ellagic acid xyloside ([M-H]− 461.0739) were tentatively characterized. Di-methyl ellagic acid xyloside was also present in fraction 4 and could strongly contribute to the antimycobacterial effect of this fraction. Additionally, protocatechuic acid ([M-H]− at m/z 153.0196) was present in fraction 4. Our antimycobacterial results obtained from this research justify the use of A. leiocarpa in Sudanese folk medicine against cough related to TB. Roots, stem bark, and leaves of A. leiocarpa are sources for new potent anti-TB drug lead compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Potential Anti-Tuberculosis Activity of the Extracts and Their Active Components of Anogeissus leiocarpa (DC.) Guill. and Perr. with Special Emphasis on Polyphenols

et al. 2020

View full text Add to dashboard Cite

show abstract

“…This difference is thought to be due to the influence of the compounds contained in the tannin extracts. The antibacterial activity of tannic plant extract on food and animal pathogenic bacteria has been determined by several studies and there is no literature study on plant pathogenic bacteria (Doss et al, 2009;Salih et al, 2017). Bektas et al (2020) demonstrated tannins can be used as an alternative to synthetic compounds in plant disease agents management.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Ortamda Biber Leke Hastalık Etmeni Xanthomonas spp.’e Karşı Bazı Tanen Ekstraktlarının Antibakteriyel Aktivitesinin Değerlendirilmesi

Küsek

İMECİK

CEYHAN

et al. 2022

Ziraat Fakültesi Dergisi

View full text Add to dashboard Cite

Biber endüstriyel olarak önemli bir kültür bitkisidir. Ülkemizde biber yetiştiriciliğinde önemli sorunlara sebep olan hastalık etmenlerinden birisi; Xanthomonas spp. cinsi bakterilerdir. Tanenler, genellikle bitkilerin kök, odun, kabuk, yaprak ve meyvelerinde bulunan ve funguslar, mayalar, bakteriler ve bazı virüsler üzerine önemli düzeyde antimikrobiyal etkileri olan bileşiklerdir. Bu çalışmada 3 farklı tanen ekstraktının (Farmatan, Artutan ve Artutan K) 4 farklı konsantrasyonun (0.5, 1, 2, ve 3 mg ml-1) biber bakteriyel leke hastalığına sebep olan ve Kahramanmaraş ili biber tarlalarından izole edilen 11 Xanthomonas spp. bakteri izolatlarına karşı antibakteriyel etkinliğini belirlemek ve etmenin mücadelesindeki önemini ortaya çıkarmak amaçlanmıştır. Disk difüzyon yönteminde oluşan zon çapına göre 3 tanen ekstraktından Farmatan ve Artutan’nın Xanthomonas spp. izolatlarına karşı antibakteriyel etkiye sahip olduğu ancak ArtutanK’nın etkinliğinin az olduğu belirlenmiştir. Her üç tanen ekstraktına karşı en duyarlı izolat ZI364 ve ZI370 olarak belirlenmiştir. Farmatan’a karşı ZI383 izolatı, Artutana’a karşı ise ZI94, ZI95, ZI107 ve ZI110 izolatları dayanıklı olduğu belirlenmiştir. Ayrıca 1 mg ml-1 konsantrasyon uygulamasında etkinin görüldüğü ve konsantrasyon arttıkça etkinin o oranda artmadığı belirlenmiştir. Sonuç olarak, uygun konsantrasyon ve duyarlı izolatların kombinasyon uygulamaları yapıldığında antibiyotiklere nazaran daha iyi etkinlik sağlanabileceği düşünülmektedir.

show abstract

Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF

Cited by 2 publications

References 0 publications

Potential Anti-Tuberculosis Activity of the Extracts and Their Active Components of Anogeissus leiocarpa (DC.) Guill. and Perr. with Special Emphasis on Polyphenols

Potential Anti-Tuberculosis Activity of the Extracts and Their Active Components of Anogeissus leiocarpa (DC.) Guill. and Perr. with Special Emphasis on Polyphenols

In Vitro Ortamda Biber Leke Hastalık Etmeni Xanthomonas spp.’e Karşı Bazı Tanen Ekstraktlarının Antibakteriyel Aktivitesinin Değerlendirilmesi

Contact Info

Product

Resources

About