Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic Weissella cibaria, CMU

Abstract

Recently, studies have explored the use of probiotics like the Weissella cibaria strain, CMU (oraCMU), for use as preventive dental medicine instead of chemical oral care methods. The present study was conducted to investigate the antibacterial properties of the cell-free supernatant (CFS) from this bacterium. Cell morphology using the scanning electron microscope, and the antibacterial effect of CFS under various growth conditions were evaluated. The production of hydrogen peroxide, organic acids, fatty acids, and secretory proteins was also studied. Most of the antibacterial effects of oraCMU against periodontal pathogens were found to be acid- and hydrogen peroxide-dose-dependent effects. Lactic acid, acetic acid, and citric acid were the most common organic acids. Among the 37 fatty acids, only 0.02% of oleic acid (C18:1n-9, cis) was detected. Proteomic analysis of the oraCMU secretome identified a total of 19 secreted proteins, including N-acetylmuramidase. This protein may be a potential anti-microbial agent effective against Porphyromonas gingivalis.

Effects of probiotic bacterium Weissella cibaria CMU on periodontal health and microbiota: a randomised, double-blind, placebo-controlled trial

“…This strain is known to inhibit caries by converting the insoluble glucan of S. mutans, a caries-inducing bacterium, into water-soluble dextran [14]. Furthermore, this strain has been reported to exhibit in vitro antimicrobial activity against Aggregatibacter actinomycetemcomitans, F. nucleatum, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola [17]. In addition, the W. cibaria strains have been reported to be advantageous due to their competitive attachment to oral epithelial cells with periodontal pathogens in the oral cavity [16].…”

“…W. cibaria CMU has been reported to inhibit the production of interleukin (IL)-6 and IL-8, which are pro-inflammatory cytokines induced by periodontal bacteria such as Fusobacterium nucleatum in oral epithelial cells [16]. Hydrogen peroxide and organic acids (e.g., lactic acid, acetic acid, and citric acid) from W. cibaria CMU have been known to be involved in antimicrobial activity [17].…”

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

“…[ 49 , 50 ], used the CFS of Bacillus species to inhibit the growth of bacterial pathogens. [ 51 ], reported the antimicrobial activities of various antimicrobial compounds which include lactic acid, acetic acid and H 2 O 2 in the CSF of Weissella cibaria against malodor inducing bacteria like Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia .…”

Weissella cibaria Attenuated LPS-Induced Dysfunction of Intestinal Epithelial Barrier in a Caco-2 Cell Monolayer Model

“…Considering W. cibaria a relatively recent member of LAB, the occurrence of potential probiotic strains belonging to this species still needs to be explored. Indeed, some specific W. cibaria strains had shown some beneficial effects, demonstrating antioxidant (Ye et al, 2018;Zhu et al, 2018), antimicrobial (Lim et al, 2018), anticancer (Kwak et al, 2018), and immunomodulatory features (Lee et al, 2018;Singh et al, 2018). Singh et al (2018) indicated that W. cibaria 28, a strain isolated from human infant feces, can prevent pro-inflammatory stress in Caco-2 cells challenged with LPS.…”