Characterization of anti‐Müllerian hormone in a case of bovine male pseudohermaphroditism

Abstract

Contents The current report aimed to characterize plasma anti‐Müllerian hormone (AMH) in bovine male pseudohermaphroditism. The blood AMH concentration in a Japanese Black male pseudohermaphrodite calf was compared with pre‐ and post‐pubertal male and female calves and castrated calves. The concentration in the case was higher than in post‐pubertal males, castrated males, and pre‐ and post‐pubertal female calves (p < .05), but similar to that in pre‐pubertal male calves. After extraction of the testes, the concentration in the case dropped to a certain extent. The extracted testes expressed AMH, as detected by immunohistochemistry. This study is the first to show the characterization of AMH in a male pseudohermaphrodite calf. AMH levels in peripheral blood might be useful to diagnose male pseudohermaphroditism in cattle.

Applying real-time quantitative PCR to diagnosis of freemartin in Holstein cattle by quantifyingSRYgene: a comparison experiment

“…A rapid, sensitive, and inexpensive method for identification of freemartin at birth or early age becomes an urgent need to reduce unnecessary economic losses and to preserve important hereditary material, also to avoid the delivery of detrimental genetic materials by progeny ( Biswas et al, 2015 ). Many diagnostic methods have been established for identification of freemartin, such as measurement of vaginal length ( Khan & Foley, 1994 ), blood grouping test for degree of hemolysis ( Kästli & Hall, 1978 ), karyotype analysis for XX/XY chimera ( Dunn, Johnson & Quaas, 1981 ), polymerase chain reaction (PCR) or improved PCR techniques for detection of specific fragments located on the Y chromosome ( Hirayama et al, 2007 ; Ron et al, 2010 ; Ayalavaldovinos et al, 2014 ), fluorescence in situ hybridization technique for detection of Y chromosome ( Sohn et al, 2007 ; Villagómez & Pinton, 2008 ; Rubes et al, 2009 ), quantitative detection of hormones such as progesterone, estradiol, and anti-Müllerian hormone ( Rota et al, 2002 ; Cabianca et al, 2007 ; Remnant et al, 2014 ; Kitahara et al, 2018 ), and detection of H-Y antigen qualitatively ( Wachtel et al, 1980 ). Most of the methods are based on the assumption that freemartin contains XX/XY chimera and H-Y antigen, whereas fertile heterosexual twin female does not contain XX/XY chimera or H-Y antigen.…”

Severe Degenerative Changes in Cryptorchid Testes in Japanese Black Cattle

“…The sensitivity and the intra-assay coefficients of variation were 11 pg/ml and <3% in the AMH assay and 0.05 ng/ml and <10% in the testosterone assay. 14 If the plasma AMH and testosterone were not detected, the value was defined as the lower limit of detection (sensitivity).…”

Endocrinological characterization of an ovarian sex cord–stromal tumor with a Sertoli cell pattern in a Japanese Black cow

“…The plasma was harvested and stored at −30°C until assayed for AMH, ir‐INH, E 2 , testosterone (T) and progesterone (P 4 ) concentrations. Plasma AMH concentrations were determined using ELISA kit (#A73818 and A73819; Beckman Coulter) as previously validated and described for cattle (El‐Sheikh Ali, Kitahara, Nibe et al, , El‐Sheikh Ali et al, ; Kitahara et al, ). The limit of quantification (LOQ) and the intra‐ and inter‐assay coefficients of variation AMH assay were 0.08 ng/ml, <6% and <10%, respectively.…”