Characterization of an Iridescent Virus Isolated from Gryllus bimaculatus (Orthoptera: Gryllidae)

Just, Frank; Eßbauer, Sandra

doi:10.1006/jipa.2000.4985

Cited by 24 publications

(33 citation statements)

References 33 publications

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“…We found no evidence of infection of the testes, supporting the hypothesis that this virus is not vertically transmitted (Williams et al, 2005). Furthermore, Just and Essbauer (Just and Essbauer, 2001) found no sign of infection in the ovaries and testes of a related cricket (Gryllus bimaculatus) infected with the same virus. Therefore, host sexual behaviour may be important for transmission under natural conditions.…”

Section: Discussionsupporting

confidence: 78%

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A viral aphrodisiac in the cricket Gryllus texensis

Adamo

Kovalko

Easy

et al. 2014

Journal of Experimental Biology

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We identified the insect iridovirus IIV-6/CrIV as a pathogen of the cricket Gryllus texensis using electron microscopy (EM) and polymerase chain reaction (PCR) analysis. EM showed that the virus attacks the fat body, an organ important for protein production, immune function and lipid storage. During infection the fat body hypertrophied, but egg production withered, leaving the lateral oviducts empty of eggs; the females were effectively sterile. EM of the testis of infected males suggests that the testis was not invaded by the virus, although sperm taken from the spermatophores of infected males showed little or no motility. Nevertheless, males and females continued to mate when infected. In fact, infected males were quicker to court females than uninfected controls. The virus benefits from the continued sexual behaviour of its host; transmission studies show that the virus can be spread through sexual contact. Sickness behaviour, the adaptive reduction of feeding and sexual behaviour that is induced by an activated immune system, was absent in infected crickets. Total haemolymph protein was reduced, as was phenoloxidase activity, suggesting a reduction in immune protein production by the fat body. The evidence suggests that during IIV-6/CrIV infection, the immune signal(s) that induces sickness behaviour is absent. Curtailment of a host's sickness behaviour may be necessary for any pathogen that is spread by host sexual behaviour.

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Section: Discussionsupporting

confidence: 78%

“…One type of iridovirus [IIV-6; cricket iridovirus isolate (CrIV) (Jakob et al, 2002)] attacks crickets. The fat body is the primary target (Just and Essbauer, 2001). It takes on an iridescent blue sheen, due to the large number of viral particles that are arranged in a paracrystalline array (Williams et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

A viral aphrodisiac in the cricket Gryllus texensis

Adamo

Kovalko

Easy

et al. 2014

Journal of Experimental Biology

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“…They had a trilaminar appearance with one electron-lucent membrane in the middle surrounded by two electron-dense membranes. Their composition was similar to that of the inner shell of Gryllus bimaculatus iridescent virus (GbIV) which was isolated from Gryllus bimaculatus (Just and Essbauer, 2001). Some five-sided particles that were continuous with membrane were observed beside the membranous materials.…”

Section: Electron Micrographs Of Amorphous Structures Within Viromamentioning

confidence: 67%

“…The family iridoviridae, which is divided into four genera Ranavirus, Lymphocystivirus, Iridovirus and Chloriridovirus, is also large (120-350 nm) DNA-containing viruses (Just and Essbauer, 2001;Tordo et al, 2004), but the viromatrix of Ranavirus has not been described in detail (Murti and Goorha, 1983;Eaton et al, 1991). In the present study, electron microscopic investigations were carried out on the ultrastructure of viromatrix of RGV in infected epithelipma papulosum cyprini (EPC) cells and relationship between the viromatrix and some cellular components.…”

Section: Introductionmentioning

confidence: 86%

Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line

Huang

Yuan

et al. 2006

Journal of Virological Methods

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“…The isolated viruses were named cricket iridovirus (CrIV) 6 and G. bimaculatus virus (GbIV). 5 Both were found to be related to IIV-6, the type species of the genus Iridovirus. In infection studies on the host range of CrIV, this isolate was successfully transmitted to other Orthopteran species, which included G. bimaculatus.…”

Section: Introductionmentioning

confidence: 99%

Experimental Infection of Crickets (Gryllus Bimaculatus) with an Invertebrate Iridovirus Isolated from a High-Casqued Chameleon (Chamaeleo Hoehnelii)

et al. 2007

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Abstract. Invertebrate iridoviruses (IIV) have been a regular problem for insect breeders. They have also recently been isolated from various lizard species. An iridovirus isolated from several tissues of a high-casqued chameleon (Chamaeleo hoehnelii) was identified as an IIV on the basis of electron microscopy (EM), sequencing of a portion of the major capsid protein (MCP) gene, and restriction endonuclease analysis of viral DNA. The pathogenicity of this isolate for crickets of the species Gryllus bimaculatus was tested by using 3 experimental infection studies. The mortality rates in the infected crickets ranged between 20% and 35%. The fat bodies of the crickets were examined on cell culture, with a nested PCR targeting the MCP gene, histologically, with in situ hybridization and by EM. Nested PCR was the most sensitive method for detecting IIV in the fat-body samples. Virus was re-isolated from several fat-body samples. In some fat bodies of infected crickets, massive arrays of viruses could be detected by EM. These findings support the hypothesis that IIV from insects are able to infect reptiles.

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Characterization of an Iridescent Virus Isolated from Gryllus bimaculatus (Orthoptera: Gryllidae)

Cited by 24 publications

References 33 publications

A viral aphrodisiac in the cricket Gryllus texensis

A viral aphrodisiac in the cricket Gryllus texensis

Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line

Experimental Infection of Crickets (Gryllus Bimaculatus) with an Invertebrate Iridovirus Isolated from a High-Casqued Chameleon (Chamaeleo Hoehnelii)

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