Characterization of an intravenous lipopolysaccharide inflammation model in calves with respect to the acute-phase response

Plessers, Elke; Wyns, Heidi; Watteyn, Anneleen; Pardon, Bart; Backer, Patrick De; Croubels, Siska

doi:10.1016/j.vetimm.2014.11.005

Cited by 33 publications

(24 citation statements)

References 55 publications

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“…Upon immunoactivation, the liver increases production of positive acute phase proteins such as haptoglobin, SAA, and LBP, which aid in immune system responses and endotoxin detoxification (Ceciliani et al, 2012). The increase in acute phase proteins is consistent with other ruminant literature; however, substantial variation is present in baseline values and magnitudes of change, particularly for LBP (Schroedl et al, 2001;Carroll et al, 2009;Plessers et al, 2015;Kvidera et al, 2017). This is likely due to differences in models, route of LPS administration, laboratory procedures, or a combination of these, but the directional change remains consistent.…”

Section: Discussionsupporting

confidence: 74%

Impacts of chronic and increasing lipopolysaccharide exposure on production and reproductive parameters in lactating Holstein dairy cows

Dickson

Kvidera

Horst

et al. 2019

Journal of Dairy Science

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Lipopolysaccharide (LPS) administration causes immunoactivation, which negatively affects production and fertility, but experimental exposure via an acute bolus is unlikely to resemble natural infections. Thus, the objectives were to characterize effects of chronic endotoxemia on production parameters and follicular development in estrous-synchronized lactating cows. Eleven Holstein cows (169 ± 20 d in milk; 681 ± 16 kg of body weight) were acclimated to their environmental surroundings for 3 d and then enrolled in 2 experimental periods (P). During P1 (3 d) cows consumed feed ad libitum and baseline samples were obtained. During P2 (7 d), cows were assigned to continuous infusion of either (1) saline-infused and pair-fed (CON-PF; 40 mL/h of saline i.v.; n = 5) or (2) LPS infused and ad libitum fed (LPS-AL; Escherichia coli O55:B5; 0.017, 0.020, 0.026, 0.036, 0.055, 0.088, and 0.148 μg/kg of body weight/h i.v. on d 1 to 7, respectively; n = 6). Controls were pair-fed to the LPS-AL group to eliminate confounding effects of dissimilar nutrient intake. Infusing LPS temporally caused mild hyperthermia on d 1 to 3 (+0.49°C) relative to baseline. Dry matter intake of LPS-AL cows decreased (28%) on d 1 of P2, then progressively returned to baseline. Relative to baseline, milk yield from LPS-AL cows was decreased on d 1 of P2 (12%). No treatment differences were observed in milk yield during P2. Follicular growth, dominant follicle size, serum progesterone (P4), and follicular P4 and 17β-estradiol concentrations were similar between treatments. Serum 17β-estradiol tended to increase (115%) and serum amyloid A and LPS-binding protein were increased (118 and 40%, respectively) in LPS-AL relative to CON-PF cows. Compared with CON-PF, neutrophils in LPS-AL cows were initially increased (45%), then gradually decreased. In contrast, monocytes were initially decreased (40%) and progressively increased with time in the LPS-AL cows. Hepatic mRNA abundance of cytochrome P450 family 2 subfamily C (CYP2C) or CYP3A was not affected by LPS, nor was there a treatment effect on toll-like receptor 4 or LBP; however, acyloxyacyl hydrolase and RELA subunit of nuclear factor kappa B tended to be increased in LPS-AL cows. These data suggest lactating dairy cows become tolerant to chronic and exponentially increasing LPS infusion in terms of production and reproductive parameters.

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Section: Discussionsupporting

confidence: 74%

Impacts of chronic and increasing lipopolysaccharide exposure on production and reproductive parameters in lactating Holstein dairy cows

Dickson

Kvidera

Horst

et al. 2019

Journal of Dairy Science

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“…The tachycardia present in these animals was similar to that described by Yates et al (2011), who found increases in heart rate in sheep treated with 1.5 μg kg -1 of LPS. Plessers et al (2015) had similar results in calves treated with doses of 0.5 μg kg -1 LPS. Figure 1.…”

Section: Resultssupporting

confidence: 65%

“…A LPS is a glycolipid molecule that is attached to the outer membrane of Gram-negative bacteria, and plays an important role in the activation of the immune system to constitute the most important surface antigen of this type of bacteria.It has a potent endotoxic action and is responsible for septic shock induced by these of pathogens (HURTA-DO; IREGUI, 2010). Escherichia coli LPS has been widely used as an alternative model for infections, because of its potent pyretic and immuno-stimulatory capacity in animals (MORGAN et al, 2008).In addition, it has been used to create reproducible systems for the study of the pathogenesis of sepsis (BURAS;HOLZMANN;SITKOVSKY, 2005), since this endotoxin generates characteristic clinical signs of this phenomenon such as fever, tachycardia and leukocyte activation (PLESSERS et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

CLINICAL AND HEMATOLOGICAL CHANGES IN SHEEP INDUCED BY Escherichia coli LIPOPOLYSACCHARIDE

Riquelme¹,

Cazanga²,

Palma³

et al. 2018

ArqVet

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Organic response to infection is characterized by a systemic reaction known as acute phase response (APR). In order to know the effect of the administration of Escherichia coli lipopolysaccharide (LPS) on physiological, hematological and biochemical variables, 10 sheep weighing 45 ± 5 kg were divided in two groups: Experimental group treated with 3 doses of 1 μg.kg-1 LPS and control group treated with saline solution (SS) at the same frequency as experimental group. Body temperature (BT°), heart rate (HR) and respiratory rate (RR) were monitored. Blood samples for hemogram and enzyme activity for aspartate amino transferase (AST) and gamma glutamyl transferase (GGT) were collected between 1 and 24 h post-LPS.LPS-treated sheep presented mean values of BT°, HR and RR higher than those observed in control sheep. At 4 and 8 hours post-injection (hpi) of LPS the leukocyte count was associated with lymphopenia, followed by leukocytosis at 24 hours. No changes were observed in the activity of AST and GGT enzymes. The results characterize APR induced by LPS in sheep, representing a useful model to study cardiovascular, hematological and biochemical responses to infection.

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“…Unlike poultry and rodents, pigs and cattle are relatively sensitive to LPS administration (Olson et al, 1995;Schmidhammer et al, 2006;Poli-de-Figueiredo et al, 2008). While in pigs and cattle LPS doses of 25 and 2.5 g/kg body weight (BW), respectively, can be considered as high; doses as high as 2500 and 20,000 g/kg BW have been applied in broiler chickens and rodents, respectively (Gerros et al, 1993;De Boever et al, 2010;Purswani et al, 2002;Zhang et al, 2008;Plessers et al, 2015). Doses of 500 and 2500 g/kg have been proven to be lethal in pigs (Schrauwen et al, , 1986Schrauwen and Houvenaghel, 1985).…”

Section: Lps Challengementioning

confidence: 99%

In vivo porcine lipopolysaccharide inflammation models to study immunomodulation of drugs

Wyns

Plessers

Backer

et al. 2015

Veterinary Immunology and Immunopathology

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Characterization of an intravenous lipopolysaccharide inflammation model in calves with respect to the acute-phase response

Cited by 33 publications

References 55 publications

Impacts of chronic and increasing lipopolysaccharide exposure on production and reproductive parameters in lactating Holstein dairy cows

Impacts of chronic and increasing lipopolysaccharide exposure on production and reproductive parameters in lactating Holstein dairy cows

CLINICAL AND HEMATOLOGICAL CHANGES IN SHEEP INDUCED BY Escherichia coli LIPOPOLYSACCHARIDE

In vivo porcine lipopolysaccharide inflammation models to study immunomodulation of drugs

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