CLINICAL AND HEMATOLOGICAL CHANGES IN SHEEP INDUCED BY Escherichia coli LIPOPOLYSACCHARIDE

Abstract

Organic response to infection is characterized by a systemic reaction known as acute phase response (APR). In order to know the effect of the administration of Escherichia coli lipopolysaccharide (LPS) on physiological, hematological and biochemical variables, 10 sheep weighing 45 ± 5 kg were divided in two groups: Experimental group treated with 3 doses of 1 μg.kg-1 LPS and control group treated with saline solution (SS) at the same frequency as experimental group. Body temperature (BT°), heart rate (HR) and respiratory rate (RR) were monitored. Blood samples for hemogram and enzyme activity for aspartate amino transferase (AST) and gamma glutamyl transferase (GGT) were collected between 1 and 24 h post-LPS.LPS-treated sheep presented mean values of BT°, HR and RR higher than those observed in control sheep. At 4 and 8 hours post-injection (hpi) of LPS the leukocyte count was associated with lymphopenia, followed by leukocytosis at 24 hours. No changes were observed in the activity of AST and GGT enzymes. The results characterize APR induced by LPS in sheep, representing a useful model to study cardiovascular, hematological and biochemical responses to infection.

LPS ile Indüklenen Septisemik Koyunlarda Bazı Eser Element Konsantrasyonlarının İncelenmesi

“…Bacterial infections are one of the major factors reducing farm animal productivity and raising morbidity and mortality rates. When the immune response, which is a defense mechanism against these infections, becomes unbalanced and cannot be successfully controlled, it can lead to the development of sepsis, which is defined as organ dysfunction resulting from the host's irregular immunological response to the infection (Riquelme et al 2018, Türkmen 2017. Lipopolysaccharide (LPS), a bacterial endotoxin, is a glycolipid molecule found in the outer membrane of gram-negative bacteria, such as Escherichia coli (E. coli), and plays a significant role in the pathophysiology of septic shock (Riquelme et al 2018).…”