Characterization of an interleukin-15 like (IL-15L) gene from zebrafish (Danio rerio)

Gunimaladevi, I.; Savan, Ram; Satô, Kenji; Yamaguchi, Ryoji; Sakai, Masahiro

doi:10.1016/j.fsi.2006.05.009

Cited by 45 publications

(31 citation statements)

References 44 publications

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“…Cytokines encompass a large and diverse family of polypeptide regulators that are secreted by specific cells of the immune system and play important roles in initiating and regulating the inflammatory processes essential for innate immunity [40]. In this study, the expression of IL-21 and type I IFN was detected.…”

Section: Discussionmentioning

confidence: 84%

“…In this study, the expression of IL-21 and type I IFN was detected. It has been reported that IL-2, IL-15 and IL-21 are closely related, signal through a common receptor and have similar biological activities [40]. With respect to IFNs, these proteins induce vertebrate cells into a state of antiviral activity and activate transcriptional regulation of several hundred IFN-stimulated genes [41].…”

Section: Discussionmentioning

confidence: 99%

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Ontogenetic expression and 17β-estradiol regulation of immune-related genes in early life stages of Japanese medaka (Oryzias latipes)

Sun

Shao

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Ontogenetic expression and 17β-estradiol regulation of immune-related genes in early life stages of Japanese medaka (Oryzias latipes)

Sun

Shao

et al. 2011

Fish & Shellfish Immunology

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“…Motifs important for downstream pathways are also conserved on several receptor subunits, including the conserved STAT5 docking sites (YXXV/L/M) on Il-2rb, Il-4ra, and Il-7ra (87)(88)(89), and a docking site for SHC, which contributes to activation of the PI3K and ERK pathways, on Il-2rb (64) (data not shown). Ligands of the IL-2R family identified in zebrafish include Il-4, Il-15, Il-15-like, and Il-21 (29,90), with potential Il-2 (91-93) and Il-7 ligands (94) characterized in other teleosts, but not Il-9 (95). Therefore, the phenotypes observed following knockdown of il-2rgc.a and jak3 could potentially be due to defective signaling via several cytokines.…”

Section: Discussionmentioning

confidence: 99%

Conserved IL-2Rγc Signaling Mediates Lymphopoiesis in Zebrafish

Sertori

Liongue

Basheer

et al. 2016

The Journal of Immunology

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The IL-2 receptor γ common (IL-2Rγc) chain is the shared subunit of the receptors for the IL-2 family of cytokines, which mediate signaling through JAK3 and various downstream pathways to regulate lymphopoiesis. Inactivating mutations in human IL-2Rγc result in SCID, a primary immunodeficiency characterized by greatly reduced numbers of lymphocytes. This study used bioinformatics, expression analysis, gene ablation, and specific pharmacologic inhibitors to investigate the function of two putative zebrafish IL-2Rγc paralogs, il-2rγc.a and il-2rγc.b, and downstream signaling components during early lymphopoiesis. Expression of il-2rγc.a commenced at 16 h post fertilization (hpf) and rose steadily from 4–6 d postfertilization (dpf) in the developing thymus, with il-2rγc.a expression also confirmed in adult T and B lymphocytes. Transcripts of il-2rγc.b were first observed from 8 hpf, but waned from 16 hpf before reaching maximal expression at 6 dpf, but this was not evident in the thymus. Knockdown of il-2rγc.a, but not il-2rγc.b, substantially reduced embryonic lymphopoiesis without affecting other aspects of hematopoiesis. Specific targeting of zebrafish Jak3 exerted a similar effect on lymphopoiesis, whereas ablation of zebrafish Stat5.1 and pharmacologic inhibition of PI3K and MEK also produced significant but smaller effects. Ablation of il-2rγc.a was further demonstrated to lead to an absence of mature T cells, but not B cells in juvenile fish. These results indicate that conserved IL-2Rγc signaling via JAK3 plays a key role during early zebrafish lymphopoiesis, which can be potentially targeted to generate a zebrafish model of human SCID.

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“…Many of these are interleukins (ILs), for which 35 different genes have been characterized in mammals [3,4]. To date, within teleosts, IL-1, -2, -4, -6, -7, -8, -10, -11, -12, -13, -15, -16, -17, -18, -19, -20, -21, -22 and -26 [2,5e9] have been isolated, together with some that appear to be fish-specific ILs such as IL-15 [10] and IL-17 [11]. A number of ILs may still remain to be discovered in fish, many of which act on T and B cells and are involved in the adaptive immune response.…”

Section: Introductionmentioning

confidence: 99%