Characterization of an Established Line of Canine Kidney Cells (MDCK).

Gaush, Charles R.; Hard, Walter L.; Smith, Thomas F.

doi:10.3181/00379727-122-31293

Cited by 295 publications

(179 citation statements)

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“…Taken together, these data suggest that renal epithelial cell lines have an ET processing mechanism different from that of endothelial cells, possibly by other proteolytic enzymes responsible for further processing of big ET, other than ET-converting enzyme. The MDCK cell line retains many of the differentiated properties typical of renal tubular cells in the loop of Henle or in the distal tubule [4][5][6], whereas the LLCPK1 cell line exhibits some of the properties of proximal tubular cells [7][8][9]. We observed dome formations in all confluent cultures of the two cell lines, suggesting functional plasma membrane polarization with the apical (microvillus) cell surface in contact with the medium and the formation of occluding junctions.…”

Section: Discussionmentioning

confidence: 89%

Secretion of endothelin and related peptides from renal epithelial cell lines

et al. 1989

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Using specific radioimmunoassays (RIAs) for endothelin (ET) and big ET, we have studied whether ET and related peptides are secreted from renal epithelial cell lines (LLCPK 1 and MDCK) of non-endothelial origin. Dilution curves of extracts of conditioned media from both LLCPK~ and MDCK cell lines were parallel to those of standard porcine (p) ET and big pET in each RIA. Both cell lines incubated in serum-free medium secreted ET-and C-terminal fragment (CTF)-like immunoreactivity (LI) of big ET as a function of time. Reverse-phase HPLC coupled with both RIAs of the extracted media from both cell lines revealed a single component with ET-LI coeluting with pET(I-21) and several components with CTF-LI, one corresponding to the elution position of big pET(I-39), one to its CTF(22-39), and the others eluting earlier than CTF. These data indicate that endothelin and related peptides are synthesized by and secreted from cells other than endothelial cells.

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Section: Discussionmentioning

confidence: 89%

Secretion of endothelin and related peptides from renal epithelial cell lines

et al. 1989

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“…Several different MDCK cell sublines exist, most of which exhibit an epithelial-like phenotype [13]. Starting from such a subline, that is MDCK 666, we have previously 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Page | 5…”

Section: Introductionmentioning

confidence: 99%

Canine MDCK cell lines are refractory to infection with human and mouse prions

Polymenidou

Trusheim

Stallmach

et al. 2008

Vaccine

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“…These cells are known for their ability to exhibit contact inhibition and achieve characteristic epithelial morphology in culture (30,31) thus providing an excellent model system for in vitro study of epithelial tissue dynamics (32)(33)(34). Using long-term fluorescence and phase-contrast video microscopy in conjunction with image segmentation and cell tracking, we have characterized the temporal progression of contact inhibition in growing MDCK colonies.…”

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confidence: 99%

Collective and single cell behavior in epithelial contact inhibition

Puliafito

Hufnagel

Neveu

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

417

473

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Control of cell proliferation is a fundamental aspect of tissue physiology central to morphogenesis, wound healing, and cancer. Although many of the molecular genetic factors are now known, the system level regulation of growth is still poorly understood. A simple form of inhibition of cell proliferation is encountered in vitro in normally differentiating epithelial cell cultures and is known as "contact inhibition." The study presented here provides a quantitative characterization of contact inhibition dynamics on tissuewide and single cell levels. Using long-term tracking of cultured Madin-Darby canine kidney cells we demonstrate that inhibition of cell division in a confluent monolayer follows inhibition of cell motility and sets in when mechanical constraint on local expansion causes divisions to reduce cell area. We quantify cell motility and cell cycle statistics in the low density confluent regime and their change across the transition to epithelial morphology which occurs with increasing cell density. We then study the dynamics of cell area distribution arising through reductive division, determine the average mitotic rate as a function of cell size, and demonstrate that complete arrest of mitosis occurs when cell area falls below a critical value. We also present a simple computational model of growth mechanics which captures all aspects of the observed behavior. Our measurements and analysis show that contact inhibition is a consequence of mechanical interaction and constraint rather than interfacial contact alone, and define quantitative phenotypes that can guide future studies of molecular mechanisms underlying contact inhibition.EMT | growth regulation | mechanics | mitosis

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Characterization of an Established Line of Canine Kidney Cells (MDCK).

Cited by 295 publications

References 0 publications

Secretion of endothelin and related peptides from renal epithelial cell lines

Secretion of endothelin and related peptides from renal epithelial cell lines

Canine MDCK cell lines are refractory to infection with human and mouse prions

Collective and single cell behavior in epithelial contact inhibition

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