Secretion of endothelin and related peptides from renal epithelial cell lines

Shichiri, Masayoshi; Hirata, Yukio; Emori, Toshiaki; Ohta, Kazuki; Nakajima, Tohru; Sato, Kengo; Sato, Atsushige; Marumo, Fumiaki

doi:10.1016/0014-5793(89)80959-7

Cited by 86 publications

(25 citation statements)

References 10 publications

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“…Concomitant immunoreactivity of ET1 and ECE1 is common to the mesangial cells [9], tubular cells [10] and vascular smooth muscle cells [11]. These sites may also be the areas of conversion of BET1 to ET1 due to the enzymatic activitiy of ECE1.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of Endothelin 1 Production in the Cochlea of Rats

Jinnouchi¹

2001

ORL

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The purpose of this study was to elucidate the mechanism of endothelin 1 (ET1) production in the cochlea of rats. Animals were anesthetized with pentobarbital sodium intraperitoneally and sacrificed by cardiac perfusion with warm saline followed by periodate-lysine-paraformaldehyde. The temporal bones and kidneys were fixed overnight in the same fixative, decalcified with 5% EDTA and embedded in paraffin. Immunohistochemical staining was performed using the labeled streptavidin biotin method to detect the localization of big endothelin 1 (BET1), ET1 and endothelin-converting enzyme 1 (ECE1). Immunoreactivity for BET1, ET1 and ECE1 was localized to the small vessels of the bony labyrinth adjacent to the spiral ligament. Immunohistochemistry for ET1 and BET1 was also localized to the spiral ligament. These results suggest that ET1 may be produced in the small vessels of the bony labyrinth adjacent to the spiral ligament and may play an important role in the cochlear function.

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Section: Discussionmentioning

confidence: 99%

Mechanism of Endothelin 1 Production in the Cochlea of Rats

Jinnouchi¹

2001

ORL

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“…These results are in agreement with in vitro and in vivo animal studies, which had identified the possible kidney sites of Et pro duction and binding. In fact, endothelial cells, epithelial kidney cell lines, mesangial and monocytic-macrophagic cells in vitro produce Et [ 10,11 ]. Et mRNA has been dem onstrated in cultured bovine glomerular endothelial cells and in the inner medulla of rats [12].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Distribution of Endothelin in Biopsies of Pediatric Nephrotic Syndrome

Murer

Zacchello²,

Basso³

et al. 1994

Am J Nephrol

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In a retrospective, double-blind study, we analyzed, utilizing an immunohistochemical technique, the distribution of Endothelin (Et) in 20 renal biopsies of 17 children with nephrotic syndrome (NS): 9 cases of steroid-responsive and 8 of steroid-resistant minimal-change disease (MCD) and 3 cases of focal segmental glomerulosclerosis (FSGS). Thirteen out of 20 biopsies (9/9 steroid-responsive MCD and 4/8 steroid-resistant MCD) showed a weak positivity of the vascular endothelial cells, as the normal renal tissue samples obtained from tumor nephrectomies. Seven out of 20 biopsies, all steroid-resistant NS, showed a marked positivity of the vascular endothelium and of the tubules and/or interstitial cells. In 4 of these 7 cases (1 MCD, 3 FSGS) a positivity of glomeruli was also revealed. The positivity of vascular endothelium seems to confirm its central role in Et production. The marked immunoreactivity detected in the endothelial cells and other renal components reveals a local increase in Et in some steroid-resistant MCD and, particularly, in FSGS. These preliminary observations suggest that Et may participate (cause or epiphenomon) in the mechanisms underlying the development of glomerulosclerosis and the progression of renal damage.

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“…Several studies described constrictions of coronary, basilar, renal, mesenteric and pulmonary arteries in various species (Furchgott & Zawadski, 1980; Le Monier de Gouville, Modot, Lippton, Hyman & Cavero, 1990). Additionally, ET release from renal epithelial cells has been demonstrated (Schichiri, Hirata, Emori, Nakajima, Sato & Marumo, 1989).…”

Section: Introductionmentioning

confidence: 99%

Endothelin and endothelium‐derived relaxing factor control of basal renovascular tone in hydronephrotic rat kidneys.

Gulbins

Hoffend

Zou

et al. 1993

The Journal of Physiology

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SUMMARY1. In order to investigate the control of renal vascular tone by endothelin (ET) and endothelium-derived relaxing factor (EDRF) under basal conditions, we infused intravenously anti-ET-1/3 antibodies (a-ET-1/3) and N-nitro-L-arginine methyl ester (L-NAME) in split hydronephrotic rat kidneys. 2. A 25 min i.v. infusion of a-ET-1/3 (4 0 x 10-3 mol kg-min-') induced a timedependent vasodilatation of arcuate (16-5 %) and interlobular arteries (18'6 %) as well as an increase of glomerular blood flow (GBF) by 32 %.3. Inhibition of EDRF synthesis by L-NAME produced a marked vasoconstriction of arcuate arteries (17 1 %) and efferent (20 1 %) arterioles and a decrease of GBF by 43 %.4. Co-infusion of a-ET-1/3 and L-NAME induced efferent vasoconstriction by 19.5 %, whereas preglomerular vessel diameters remained unchanged.5. The specificity of a-ET-1/3 effects was confirmed by simultaneous i.v. application of a-ET-1/3 and ET-1 (160 ng i.v.) which produced no significant vascular effects. Injection of ET-1 alone constricted arcuate arteries and decreased glomerular blood flow by 25 %.6. Experiments in normal rat kidneys with a-ET-1/3 I.v. revealed an increase of renal blood flow by 21 %.7. Our results demonstrate a physiological control of basal vascular tone in larger preglomerular arterioles by ET and EDRF. Efferent arteriolar tone is predominantly controlled by EDRF.

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Secretion of endothelin and related peptides from renal epithelial cell lines

Cited by 86 publications

References 10 publications

Mechanism of Endothelin 1 Production in the Cochlea of Rats

Mechanism of Endothelin 1 Production in the Cochlea of Rats

Immunohistochemical Distribution of Endothelin in Biopsies of Pediatric Nephrotic Syndrome

Endothelin and endothelium‐derived relaxing factor control of basal renovascular tone in hydronephrotic rat kidneys.

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