Characterization of an efficient estrogen-degrading bacterium Stenotrophomonas maltophilia SJTH1 in saline-, alkaline-, heavy metal-contained environments or solid soil and identification of four 17β-estradiol-oxidizing dehydrogenases

Xiong, Weiliang; Chen, Yin; Wang, Yanqiu; Lin, Shuangjun; Deng, Zixin; Liang, Rubing

doi:10.1016/j.jhazmat.2019.121616

Cited by 35 publications

(15 citation statements)

References 74 publications

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“…For example, Phyllobacterium myrsinacearum , a metal-resistant bacterium, was reported as a promising soil bioremediator, particularly when associated with hyperaccumulator plants such as Pteris vittata [ 100 ] and Sedum plumbizincicola [ 101 ]. Additionally, P. phenoliruptrix is able to degrade recalcitrant xenobiotics [ 102 ], while Pseudomonas synxantha and S. maltophili are strong candidates for bioremediation of soil contaminated seawater [ 103 ] and chromium (Cr) [ 104 ] and estrogen [ 105 ], respectively. On the other hand, Pseudomonas gessardii has been indicated as a potential biocatalyst for the synthesis of bromohydrins [ 106 ] and for enzyme therapy [ 107 ].…”

Section: Discussionmentioning

confidence: 99%

Mining the Microbiome of Key Species from African Savanna Woodlands: Potential for Soil Health Improvement and Plant Growth Promotion

et al. 2020

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(1) Aims: Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes. (2) Methods: Bacterial communities were identified through Illumina Miseq sequencing of the 16S rRNA gene amplicons, followed by culture dependent methods to isolate plant growth-promoting bacteria (PGPB). Plant growth-promoting traits of the cultivable bacterial fraction were further analyzed. To screen for the presence of nitrogen-fixing bacteria, the promiscuous tropical legume Vigna unguiculata was used as a trap host. The taxonomy of all purified isolates was genetically verified by 16S rRNA gene Sanger sequencing. (3) Results: Bacterial community results indicated that fire did not drive major changes in bacterial abundance. However, culture-dependent methods allowed the differentiation of bacterial communities between the sampled sites, which were particularly enriched in Proteobacteria with a wide range of plant-beneficial traits, such as plant protection, plant nutrition, and plant growth. Bradyrhizobium was the most frequent symbiotic bacteria trapped in cowpea nodules coexisting with other endophytic bacteria. (4) Conclusion: Although the global analysis did not show significant differences between landscapes or sites with different fire regimes, probably due to the fast recovery of bacterial communities, the isolation of PGPB suggests that the rhizosphere bacteria are driven by the plant species, soil type, and fire regime, and are potentially associated with a wide range of agricultural, environmental, and industrial applications. Thus, the rhizosphere of African savannah ecosystems seems to be an untapped source of bacterial species and strains that should be further exploited for bio-based solutions.

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Section: Discussionmentioning

confidence: 99%

Mining the Microbiome of Key Species from African Savanna Woodlands: Potential for Soil Health Improvement and Plant Growth Promotion

et al. 2020

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“…Its molecular formula was calculated as C 18 H 22 O 2 , which was determined to be E1 (estrone). In addition, E1 was formed by the dehydrogenation process at the C-17 position of the D ring of E2, and it was suggested that the formation of E1 was caused by 17β-hydroxysteroid dehydrogenases (Xiong et al, 2020). In previous studies, E1 was generally regarded as the initial step of estrogen degradation (Shi et al, 2004).…”

Section: Biodegradation Products and Metabolic Pathwaysmentioning

confidence: 99%

“…Yu et al (2016) reported that 1 mg/L of E2 could be completely degraded by Rhodococcus spp. DS201 with 1% inoculation quantity in 3 days at 30 • C. Xiong et al also claimed that a 90% removal efficiency of 10 mg/L of E2 could be achieved by the strain SJTH1 with the initial OD600 of 0.05 (Xiong et al, 2020). All these above strains could effectively degrade E2, but it was difficult to compare their degradation efficiencies due to different experimental conditions.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study on the Biodegradation of 17β-Estradiol by Candida utilis CU-2 and Lactobacillus casei LC-1

Yang

et al. 2021

Front. Energy Res.

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The release and fate of estrogens have attracted more and more public attention. Biodegradation is an important method for estrogen removal from the environment. However, few comparative studies concentrated on the degradation of 17β-estradiol (E2) by fungi and bacteria. In this study, the removal efficiencies of E2 by fungi (Candida utilis CU-2) and bacteria (Lactobacillus casei LC-1) were investigated through influencing factors, kinetics, and biodegradation pathways. The results demonstrated that both C. utilis CU-2 and L. casei LC-1 have the same degradation efficiency, and they can effectively degrade E2 (10 μM) with nearly 97% degradation efficiency. However, the biodegradation efficiency of the two strains only reached 20% when E2 was used as a sole carbon source, while it increased to 97% with 1.2 g/L sucrose, glucose, or sodium acetate supply, indicating the occurrence of co-metabolism. In addition, the results indicated that 35°C and 0.6 g/L sucrose favored the degradation. However, the addition of excessive carbon sucrose (10 g/L) significantly inhibited the biodegradation of E2. Besides, the degradation of E2 with ~0–10 g/L sucrose as co-substrate followed the first-order kinetics well. Through intermediate products analysis, 12 degradation products were identified, and they were mainly produced via hydroxylation and methylation, among others, among which C14H22O4 (m/z:[M + H]+ = 255) was detected as the product with the smallest amount of carbon in this study. Based on the detected products and previous studies, five biodegradation pathways were proposed. To our knowledge, there are few reports about the comparisons of E2 removal between fungi and bacteria. Moreover, the results confirmed that the strain CU-2 and the strain LC-1 may have similar degradation characteristics and metabolic mechanisms in the degradation of E2. This study may provide a promising bio-treatment method with low energy consumption for E2 removal from aqueous environments and help in understanding their biodegradation mechanisms.

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“…Genomic investigation of S. maltophilia SJTH1 isolated from the hospitalized patient of PGIMER, Chandigarh, India revealed diverse antibiotic resistance genes, efflux pumps, heavy metal resistance, various transcriptional regulators etc. [88]. Reports are also available on multidrug-resistant C. davisae from Indian Hospital [89].…”

Section: Page 11 / 18mentioning

confidence: 99%

Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

Nath¹,

Sinha²,

Singha³

et al. 2020

Environ Anal Health Toxicol

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Pseudomonas aeruginosa and Bacillus sp. are ubiquitous in hospital wastewater discharges. The infections caused by P. aeruginosa become complicated, as they acquire the ability to resist many classes of antibiotics. Some strains of P. aeruginosa has been reported to survive for months on dry surfaces, and it can persist and grow in contaminated antimicrobial handwash containing triclosan, making it a critical concern for hospital staff [5]. Infections with Bacillus sp. are rare, but they are able to produce spores and grow haemolytically on blood agar plates (β-hemolysis). Gram-positive Bacilli can cause many types of infections and are spread to humans in a variety of ways [6,7]. Some of the frequently encountered species include B.

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Characterization of an efficient estrogen-degrading bacterium Stenotrophomonas maltophilia SJTH1 in saline-, alkaline-, heavy metal-contained environments or solid soil and identification of four 17β-estradiol-oxidizing dehydrogenases

Cited by 35 publications

References 74 publications

Mining the Microbiome of Key Species from African Savanna Woodlands: Potential for Soil Health Improvement and Plant Growth Promotion

Mining the Microbiome of Key Species from African Savanna Woodlands: Potential for Soil Health Improvement and Plant Growth Promotion

A Comparative Study on the Biodegradation of 17β-Estradiol by Candida utilis CU-2 and Lactobacillus casei LC-1

Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

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