Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

Nath, Soumitra; Sinha, Ahana; Singha, Y. Suchitra; Dey, Ankita; Bhattacharjee, Nilakshi; Deb, Bibhas

doi:10.5620/eaht.2020018

Cited by 7 publications

(4 citation statements)

References 93 publications

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“…Hence, it is clear from the observation that with the increase in F-doping, the antibacterial properties of F-doped HAp are effectively enhanced towards the tested bacteria. Pseudomonas aeruginosa and Bacillus subtilis, a general toxic pathogen that forms biofilms and is antibiotic-resistant, may cause bone implement infections in hospitalized patients [ 68 ].…”

Section: Resultsmentioning

confidence: 99%

Citric Acid-Mediated Microwave-Hydrothermal Synthesis of Mesoporous F-Doped HAp Nanorods from Bio-Waste for Biocidal Implant Applications

Karunakaran

Cho

Kumar³

et al. 2022

Nanomaterials

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In this current research, mesoporous nano-hydroxyapatite (HAp) and F-doped hydroxyapatite (FHAp) were effectively obtained through a citric acid-enabled microwave hydrothermal approach. Citric acid was used as a chelating and modifying agent for tuning the structure and porosity of the HAp structure. This is the first report to use citric acid as a modifier for producing mesoporous nano HAp and F-doped FHAp. The obtained samples were characterized by different analyses. The XRD data revealed that F is incorporated well into the HAp crystal structure. The crystallinity of HAp samples was improved and the unit cell volume was lowered with fluorine incorporation. Transmission electron microscopy (TEM) images of the obtained samples revealed that a nano rod-like shape was obtained. The mesoporous structures of the produced HAp samples were confirmed by Brunauer–Emmett–Teller (BET) analysis. In vivo studies performed using zebrafish and C. elegans prove the non-toxic behavior of the synthesized F doped HAp samples. The obtained samples are also analyzed for antimicrobial activity using Gram-negative and Gram-positive bacteria, which are majorly involved in implant failure. The F doped samples revealed excellent bactericidal activity. Hence, this study confirms that the non-toxic and excellent antibacterial mesoporous F doped HAp can be a useful candidate for biocidal implant application.

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Section: Resultsmentioning

confidence: 99%

Citric Acid-Mediated Microwave-Hydrothermal Synthesis of Mesoporous F-Doped HAp Nanorods from Bio-Waste for Biocidal Implant Applications

Karunakaran

Cho

Kumar³

et al. 2022

Nanomaterials

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“…Many bacteria isolated from diverse sources, such as Bacillus sp. [Glibota et al, 2020;Nath et al, 2020;Alotaibi et al, 2021], Stenotrophomonas sp. [Agarwal et al, 2019;Nath et al, 2020], Chryseobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

“…[Glibota et al, 2020;Nath et al, 2020;Alotaibi et al, 2021], Stenotrophomonas sp. [Agarwal et al, 2019;Nath et al, 2020], Chryseobacterium sp. [Glibota et al, 2020], Arthrobacter sp.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Potential Virulence, Resistance to Antibiotics and Heavy Metals, and Biofilm-Forming Capabilities of Soil Lignocellulolytic Bacteria

Kognou¹,

Chio²,

Khatiwada³

et al. 2023

Microb Physiol

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Soil bacteria participate in self-immobilization processes for survival, persistence and producing virulence factors in some niches or hosts through their capacities of autoaggregation, cell surface hydrophobicity, biofilm formation, and antibiotic and heavy metal resistance. This study investigated potential virulence, antibiotics and heavy metals resistance, solvent adhesion, and biofilm-forming capabilities of six cellulolytic bacteria isolated from soil samples: Paenarthrobacter sp. MKAL1, Hymenobacter sp. MKAL2, Mycobacterium sp. MKAL3, Stenotrophomonas sp. MKAL4, Chryseobacterium sp. MKAL5 and Bacillus sp. MKAL6. Strains were subjected to phenotypic methods, including heavy metal and antibiotic susceptibility and virulence factors (protease, lipase, capsule production, autoaggregation, hydrophobicity and biofilm formation). The effect of ciprofloxacin was also investigated on bacterial susceptibility over time, cell membrane and biofilm formation. Strains MKAL2, MKAL5 and MKAL6 exhibited protease and lipase activities, while only MKAL6 produced capsules. All strains were capable of aggregating, forming biofilm and adhering to solvents. Strains tolerated high amounts of chromium, lead, zinc, nickel and manganese and were resistant to lincomycin. Ciprofloxacin exhibited bactericidal activity against these strains. Although the phenotypic evaluation of virulence factors of bacteria can indicate their pathogenic nature, an in-depth genetic study of virulence, antibiotic and heavy metal resistance genes is required.

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“…Moreover, microbiological contamination of antiseptics and disinfectants has been recorded in medical settings (Amsalu et al, 2020). Nonetheless, the environmental transmission of antibioticresistant bacteria has been established all through hospital outbreaks (Nath et al, 2020). Furthermore, antibiotic resistance has been demonstrated in nosocomial microorganisms isolates from the healthcare setting (Jain et al, 2021).…”

Section: Omermentioning

confidence: 99%

Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates

Omer

Aka

2023

Polytechnic j.

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Background and objectives: Multi-drug resistant microorganisms have caused a remarkable increase in hospital-acquired infections (HAI) during the previous few decades. Moreover, microbiological contamination of antiseptics and disinfectants has been recorded in medical settings. Methods: A total of 61 clinically relevant Acinetobacter baumannii isolates were collected from various clinical specimens in hospitals in Erbil city/Iraq between September 2021 and March 2022. The isolates were identified using VITEK-2 compact system. Antimicrobial susceptibility testing was carried out for a total of 16 different therapeutically relevant antibiotics in accordance with the criteria of the Clinical Laboratory Standards Institute (CLSI) using the above mentioned system. A spectrophotometer was used to determine the optical density at 600 nm (OD600nm) for biofilm evaluation. Wilcoxon signed ranks test used to determine the effect of sub-MIC of CHX on the CHX-MIC and biofilm formation. Results: the study showed that there is a significant difference in CHX-MIC and biofilm formation before and after 7 days of incubation of Acinetobacter baumannii in sub-MIC of CHX (p<0.05). Exposure of Acinetobacter baumannii to sub-lethal CHX concentrations develops inducible resistance to lethal CHX doses. The biofilm formation ability of isolates also increased after using sub-lethal dose of CHX.

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Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

Cited by 7 publications

References 93 publications

Citric Acid-Mediated Microwave-Hydrothermal Synthesis of Mesoporous F-Doped HAp Nanorods from Bio-Waste for Biocidal Implant Applications

Citric Acid-Mediated Microwave-Hydrothermal Synthesis of Mesoporous F-Doped HAp Nanorods from Bio-Waste for Biocidal Implant Applications

Characterization of Potential Virulence, Resistance to Antibiotics and Heavy Metals, and Biofilm-Forming Capabilities of Soil Lignocellulolytic Bacteria

Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates

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