Characterization of an attenuated TE3L-deficient vaccinia virus Tian Tan strain

Wang, Yuhang; Kan, Shihai; Du, Shouwen; Qi, Yanxin; Wang, Jinhui; Li, Liming; Ji, Huifan; He, Dongxu; Wu, Na; Li, Chang; Chi, Baorong; Li, Xiao; Jin, Ningyi

doi:10.1016/j.antiviral.2012.10.002

Cited by 10 publications

(4 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TC7L-TK2L is not only a host-range gene but also a host defense regulatory gene (McFadden, 2005 ; Zhu et al, 2007 ; Yu et al, 2010 ). TE3L is a virulence-associated gene that inhibits the antiviral activity of interferon, and it is also associated with host range determinants, host defense regulation factors, and cell apoptosis regulatory factors (Wang et al, 2012 ). TA35R is a vaccinia A-type inclusion body protein gene fragment that is homologous with A26L of Vaccinia virus Copenhagen strain-encoded A-type envelope proteins (Rehm and Roper, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…Another study showed that the virulence decreased significantly in the absence of B13L in the WR strain in nude mice and normal mice, while humoral immunity and cellular immune response were still high (Legrand et al, 2004 ). Knockout of E3L has also been shown to inhibit interferon activation and antiviral response (Langland and Jacobs, 2002 ), which means that deletion of this gene attenuates viral virulence (Wang et al, 2012 ). Altogether, the present findings as well the findings of previous studies show that the deletion of these specific non-essential genes that are not associated with viral replication can attenuate the virulence of VV and therefore improve its prospects as a vaccine.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Generation of an Attenuated Tiantan Vaccinia Virus Strain by Deletion of Multiple Genes

Zhu

Chen

et al. 2017

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

An attenuated vaccinia virus-MVTTEAB-was constructed by deletion of non-essential gene segments related to the immunomodulatory and virulence functions of the vaccinia virus Tiantan strain (VVTT). The shuttle plasmids pTC-EGFP, pTE-EGFP, pTA35-EGFP, pTB-EGFP, and pTA66-EGFP were constructed and combined with the early and late strong promoter pE/L and EGFP as an exogenous selectable marker. Then, through the homologous recombination technology and Cre/loxP system, the following gene fragments were gradually knocked out one by one: TC7L-TK2L, TE3L, TA35R, TB13R, and TA66R. Ultimately, the five-segment-deleted attenuated strain MVTTEAB was obtained. Knockout of these segments and genetic stability of MVTTEAB were confirmed, and it was also shown that knockout of these segments did not affect the replication ability of the virus. Further, a series of in vivo and in vitro experiments demonstrated that the virulence of MVTTEAB was attenuated significantly, but at same time, high immunogenicity was maintained. These results indicate that MVTTEAB has potential for clinical use as a safe viral vector or vaccine with good attenuation and immunogenicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Generation of an Attenuated Tiantan Vaccinia Virus Strain by Deletion of Multiple Genes

Zhu

Chen

et al. 2017

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The CHIKV virus (GenBank: KU561451.1) was provided by Yunnan Institute of Parasitic Diseases, whereas the JEV virus (GenBank: JQ086762.1) was isolated in our laboratory. The TE3L deletion mutant of recombinant VACV strain TianTan (rVTT) (Wang et al 2012) and the shuttle vector pSTKE-EGFP plasmid (Du et al 2012) were previously constructed. BHK-21 cells (Baby Hamster Syrian Kidney cells) were cultured in Dulbecco's modified Eagle's medium (DMEM) with 5% fetal bovine serum (FBS) and 1% penicillin-streptomycin at 37°C in a CO 2 incubator.…”

Section: Experimental Animals Viruses Cells and Plasmidsmentioning

confidence: 99%

Construction and Immunogenicity of Recombinant Vaccinia Virus Vaccine Against Japanese Encephalitis and Chikungunya Viruses Infection in Mice

Zhang

Han

Jie

et al. 2020

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

Japanese encephalitis virus (JEV) is recognized as a public health risk by the World Health Organization. In Asia, each year, *70,000 people become infected with JEV, which results in *10,000 deaths. Chikungunya virus (CHIKV) is an RNA virus, whose infection mainly causes fever, myalgia, and skin rash. Although the mortality rate is low, it seriously affects daily life. JEV and CHIKV infect humans through mosquitoes; therefore, a recombinant vaccinia virus coexpressing JEV E and CHIKV E1 proteins was constructed to prevent their concurrent infection. In this study, after mice first immunization, booster immunization was performed at 21 days postimmunization (dpi). At 35 dpi, mice were challenged with JEV and CHIKV. Specific antibodies significantly increased in the rVTT-CE1-JE-EGFP group, which were significantly (p < 0.01) higher than those of the control groups at 35 dpi. The plaque reduction neutralization tests (JEV) of rVTT-CE1-JE-EGFP group was 1:320 at 35 dpi. Furthermore, cytokine levels and the percentage of CD3 + CD4 + and CD3 + CD8 + T-lymphocytes in the rVTT-CE1-JE-EGFP group were significantly (p < 0.01) higher than those in the control groups at 35 dpi. After challenge, mice body weights in rVTT-CE1-JE-EGFP group were not significantly altered, and the survival rate was 100%. These results showed the rVTT-CE1-JE-EGFP group elicited significant humoral and cellular immune responses, thus indicating that the recombinant vaccine may serve as a candidate for effective prevention of CHIKV and JEV infection.

show abstract

“…Although humoral immune response is generally believed to play an essential role in preventing OPXVs infection [ 2 ], we also detected the cellular immune response induced by VTT. The murine splenocytes were stimulated with heat-inactivated VTT or MPXV (10 4 PFU/well) and the IFN-γ ELISpot assay was performed [ 8 ]. The median number of IFN-γ secreting cells in mice vaccinated with 1 × 10 4 PFUs VTT reached 177 spot-forming cells (SFCs)/10 5 cells after exposure to VTT, but decreased to 19 SFCs/10 5 cells upon MPXV stimulation ( Figure 1 E).…”

mentioning

confidence: 99%

Vaccinia virus tiantan strain is inefficient in eliciting cross-reactive immunity against the emerging monkeypox virus strain

Tian,

Zhang,

Liu

et al. 2024

Emerging Microbes & Infections

View full text Add to dashboard Cite

Characterization of an attenuated TE3L-deficient vaccinia virus Tian Tan strain

Cited by 10 publications

References 46 publications

Generation of an Attenuated Tiantan Vaccinia Virus Strain by Deletion of Multiple Genes

Generation of an Attenuated Tiantan Vaccinia Virus Strain by Deletion of Multiple Genes

Construction and Immunogenicity of Recombinant Vaccinia Virus Vaccine Against Japanese Encephalitis and Chikungunya Viruses Infection in Mice

Vaccinia virus tiantan strain is inefficient in eliciting cross-reactive immunity against the emerging monkeypox virus strain

Contact Info

Product

Resources

About