Characterization of an anti‐varicella‐zoster virus compound that targets the portal protein encoded by ORF54

Yasui, Ruka; Yoshida, Chinatsu; Yamaguchi, Toshiaki; Inoue, Naoki

doi:10.1111/1348-0421.12507

Cited by 9 publications

(4 citation statements)

References 31 publications

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“…The large terminase subunit has an ATPase activity that provides energy for packaging initiation and termination [36],[37]. Portal protein (encoded by ORF77) shares high amino acid sequence similarity with Staphylococcus phage vB_SepS_SEP9, which can inject DNA into the host cell through a pathway formed by portal protein [38]. Assembly protease (encoded by ORF78) and DNA packaging protein (encoded by ORF81) were also found in phage SA2.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of the <em>Staphylococcus aureus</em> bacteriophage vB_SauS_SA2

Wang¹,

Zhao²,

Sun³

et al. 2019

AIMS Microbiology

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A novel bacteriophage vB_SauS_SA2 (hereafter designated SA2) that infects Staphylococcus aureus was isolated. At a multiplicity of infection (MOI) of 0.1, phage SA2 had a latent period of about 10 min with a burst size of 293 PFUs/infected cell (PFU, plaque forming unit). Phage SA2 had a double-stranded DNA genome with a length of 89,055 bp and a G + C content of 31.9%. The genome contained 130 open reading frames (ORFs), 28 of which had assigned functions, and 18 were unique. One tRNA gene (tRNAAsn) was discovered, and no virulence genes were identified. Its genome showed very low similarity with phage genomes deposited in public databases (75% nucleotide identity and 7% query coverage). The unique characteristics of phage SA2 led to the proposal of a new Siphoviridae genus named ‘SA2likevirus’.

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Section: Resultsmentioning

confidence: 99%

Isolation and characterization of the <em>Staphylococcus aureus</em> bacteriophage vB_SauS_SA2

Wang¹,

Zhao²,

Sun³

et al. 2019

AIMS Microbiology

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“…phi-pp2. ORF181 was predicted to encode a portal protein with 100% identity to that of Vibrio phage phi-pp2, which can infect DNA into the host cell through a pathway formed by portal protein (Yasui et al, 2017). Just as reported (Paul et al, 1998), terminase plays an important role in the DNA packing processes of the phage genome.…”

Section: Genome Sequencing Characterization and Analysis Of Phage Pva8mentioning

confidence: 84%

Characterization of vB_ValM_PVA8, a broad-host-range bacteriophage infecting Vibrio alginolyticus and Vibrio parahaemolyticus

Zhao

et al. 2023

Front. Microbiol.

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Phage therapy was taken as an alternative strategy to antibiotics in shrimp farming for the control of Vibrio species of Vibrio parahaemolyticus and Vibrio alginolyticus, which cause substantial mortality and significant economic losses. In this study, a new Vibrio phage vB_ValM_PVA8 (PVA8), which could efficiently infect pathogenic isolates of V. alginolyticus and V. parahaemolyticus, was isolated from sewage water and characterized by microbiological and in silico genomic analyses. The phage was characterized to be a member of the Straboviridae family with elongated head and contractile tail by transmission electron microscopy. Genome sequencing showed that PVA8 had a 246,348-bp double-stranded DNA genome with a G + C content of 42.6%. It harbored totally 388 putative open reading frames (ORFs), among them 92 (23.71%) assigned to functional genes. Up to 27 transfer RNA (tRNA) genes were found in the genome, and the genes for virulence, antibiotic resistance, and lysogeny were not detected. NCBI genomic blasting results and the phylogenetic analysis based on the sequences of the large terminase subunits and the DNA polymerase indicated that PVA8 shared considerable similarity with Vibrio phage V09 and bacteriophage KVP40. The phage had a latent period of 20 min and a burst size of 309 PFUs/infected cell with the host V. alginolyticus, and it was stable over a broad pH range (4.0–11.0) and a wide temperature span (−80°C to 60°C), respectively, which may benefit its feasibility for phage therapy. In addition, it had the minimum multiplicity of infection (MOI) of 0.0000001, which revealed its strong multiplication capacity. The shrimp cultivation lab trials demonstrated that PVA8 could be applied in treating pathogenic V. parahaemolyticus infection disease of shrimp with a survival rate of 88.89% comparing to that of 34.43% in the infected group, and the pond application trails confirmed that the implementation of PVA8 could rapidly yet effectively reduce the level of the Vibrio. Taken together, PVA8 may be potential to be explored as a promising biological agent for Vibrio control in aquaculture farming industry.

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“…Packaging related proteins: the terminase is a packaging protein that inserts a single phage genome within an empty capsid structure, typically consisting of one large and one small subunit, the small subunit speci cally recognizes viral DNA and attracts the large subunit for initial cleavage, the large subunit has ATPase activity and can provide energy for phage packaging initiation [26]. Phage portal protein can form a pathway to inject DNA into the host and participate in the protein connection between the phage head and tail [29].…”

Section: Transcription and Modulementioning

confidence: 99%

Complete Genome Sequence of Bacteriophage EFC1 Infecting Enterococcus Faecalis From Chicken

Wang¹,

Liu²

2021

Preprint

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In response to Enterococcus faecalis infection of chicken origin, a multi host lytic phage, EFC1 was isolated and characterized the double-stranded circular DNA genome with size of 56099 bp, containing 89 predicted protein coding genes as well as 2 tRNAs involved in intron, structure, transcription, packaging, DNA replication, modification, lysis. Observation of the structure by electron microscopy and comparative phylogenetic analysis of terminase large subunit showed that the phage EFC1 belongs to a new member of Siphoviridae, which is relatively distantly related to its high similarity phages. The phage EFC1 has no relevant virulence genes and antibiotic resistance genes.

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Characterization of an anti‐varicella‐zoster virus compound that targets the portal protein encoded by ORF54

Cited by 9 publications

References 31 publications

Isolation and characterization of the <em>Staphylococcus aureus</em> bacteriophage vB_SauS_SA2

Isolation and characterization of the <em>Staphylococcus aureus</em> bacteriophage vB_SauS_SA2

Characterization of vB_ValM_PVA8, a broad-host-range bacteriophage infecting Vibrio alginolyticus and Vibrio parahaemolyticus

Complete Genome Sequence of Bacteriophage EFC1 Infecting Enterococcus Faecalis From Chicken

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