Characterization of an alcoholic hepatic steatosis model induced by ethanol and high-fat diet in rats

Souza, Carlos Eduardo Alves de; Stolf, Aline Maria; Dreifuss, Arturo Alejandro; Lívero, Francislaine Aparecida dos Reis; Gomes, Liana de Oliveira; Petiz, Lyvia Lintzmaier; Beltrame, Olair Carlos; Dittrich, Rosângela Locatelli; Telles, José Ederaldo Queiroz; Cadena, Sílvia Maria Suter Correia; Acco, Alexandra

doi:10.1590/s1516-8913201500294

Cited by 4 publications

(3 citation statements)

References 48 publications

(43 reference statements)

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“…It has been stated that low doses of testosterone in impairment functions, lead to accumulation of lipids with large number of Sudanophilia insides the hepatocytes. 38 the present findings are in agreement with the findings of [39][40][41] who concluded that liver cells increased their affinity to Sudanophilia after treatment with testosterone.Also these findings are in concomitant with the findings of 42 who stated thatthere was a significant increase in testosterone level in castrated subject treated with testosterone that resulted in an increase of Sudanophilia of liver cells.…”

Section: Discussionsupporting

confidence: 93%

Affinity of liver cells to Sudanophilia and PAS in castrated treated mice

Mh¹,

Lo²,

Hussein³

et al. 2020

MOJAP

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Section: Discussionsupporting

confidence: 93%

Affinity of liver cells to Sudanophilia and PAS in castrated treated mice

Mh¹,

Lo²,

Hussein³

et al. 2020

MOJAP

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“…After this analysis, 223 articles were potentially eligible for the review; however, 17 were excluded owing to missing data. Therefore, 206 files [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , …”

Section: Resultsmentioning

confidence: 99%

“…A total of 206 [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ,…”

Section: Resultsunclassified

The Role of Oxidative Stress in Alcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis of Preclinical Studies

Rabelo,

Andrade,

Costa

2024

Nutrients

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Alcoholic Fatty Liver Disease (AFLD) is characterized by the accumulation of lipids in liver cells owing to the metabolism of ethanol. This process leads to a decrease in the NAD+/NADH ratio and the generation of reactive oxygen species. A systematic review and meta-analysis were conducted to investigate the role of oxidative stress in AFLD. A total of 201 eligible manuscripts were included, which revealed that animals with AFLD exhibited elevated expression of CYP2E1, decreased enzymatic activity of antioxidant enzymes, and reduced levels of the transcription factor Nrf2, which plays a pivotal role in the synthesis of antioxidant enzymes. Furthermore, animals with AFLD exhibited increased levels of lipid peroxidation markers and carbonylated proteins, collectively contributing to a weakened antioxidant defense and increased oxidative damage. The liver damage in AFLD was supported by significantly higher activity of alanine and aspartate aminotransferase enzymes. Moreover, animals with AFLD had increased levels of triacylglycerol in the serum and liver, likely due to reduced fatty acid metabolism caused by decreased PPAR-α expression, which is responsible for fatty acid oxidation, and increased expression of SREBP-1c, which is involved in fatty acid synthesis. With regard to inflammation, animals with AFLD exhibited elevated levels of pro-inflammatory cytokines, including TNF-a, IL-1β, and IL-6. The heightened oxidative stress, along with inflammation, led to an upregulation of cell death markers, such as caspase-3, and an increased Bax/Bcl-2 ratio. Overall, the findings of the review and meta-analysis indicate that ethanol metabolism reduces important markers of antioxidant defense while increasing inflammatory and apoptotic markers, thereby contributing to the development of AFLD.

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Alcoholic liver disease and intestinal microbiota in an experimental model: Biochemical, inflammatory, and histologic parameters

Thoen

Longo²,

Leonhardt³

et al. 2023

Nutrition

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Characterization of an alcoholic hepatic steatosis model induced by ethanol and high-fat diet in rats

Abstract: Alcoholic liver disease is characterized by a wide spectrum of liver damage, which increases when ethanol is associated with high-fat diets (HFD). This work aimed to establish a model of alcoholic hepatic steatosis (AHS

Cited by 4 publications

References 48 publications

Affinity of liver cells to Sudanophilia and PAS in castrated treated mice

Affinity of liver cells to Sudanophilia and PAS in castrated treated mice

The Role of Oxidative Stress in Alcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis of Preclinical Studies

Alcoholic liver disease and intestinal microbiota in an experimental model: Biochemical, inflammatory, and histologic parameters

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