Alcoholic liver disease and intestinal microbiota in an experimental model: Biochemical, inflammatory, and histologic parameters

Amelioration of Lipid Metabolic Profiles by Trigonelline a Bioactive Compound of Trigonella Foenum-graecum in Alcohol Induced Albino Rats

“…The observed significant elevation of these profiles is indicative of alcohol-induced pathological changes and oxidative stress in the liver, brain, heart and kidney. 12 Our studies are in agreement with the report of Kim et al 13 that rats fed with alcohol showed hyperlipidemia. However, with trigonelline supplementation in alcoholic rats MDA, TC and TGs levels were depleted in alcoholic rats.…”

Comparison of Gut Microbiota in Alcoholic and Metabolic-Dysfuncion Associated Steatotic Liver Disease in Animal Models

“…The increased cardiovascular risk of this MASLD experimental model was previously demonstrated by our research group, supporting the increased abundance of the Romboutsia genus (7) . Experimental models for ALD are difficult to establish as rodents have aversion to alcohol (11,33) . Other described models are expensive and difficult to reproduce because they either require special diets, infusion pumps or invasive procedures (34,35) .…”

“…However, the development of experimental models to elucidate the pathophysiological mechanisms of ALD and to evaluate potential therapeutic strategies is essential. The model developed by our group is capable of triggering ALD at an early stage with histopathological and biochemical changes in liver function that correspond to the phenotype of the disease in human (11) .The severity of alcohol-induced liver injury might be related to a specific signature of GM composition. In our study, animals exposed to alcohol abuse for eight weeks had high abundance of Cyanobacteria and Verrucomicrobiota phyla.…”

“…Animals in MASLD model exhibited marked deposition of body and hepatic fat, activation of mi-croRNAs, receptors, mediators, and inflammatory cytokines, along with an increased risk of developing cardiovascular diseases. On the other hand, animals in ALD model presented, significant alterations in body composition were demonstrated, suggesting worsening nutritional status, biochemical changes, and hepatic fat deposition in the ALC4 and ALC8 groups (7,10,11) . Regarding the histopathological evaluation of the liver in the experimental MASLD model, no hepatic abnormalities were reported in the liver tissue of the MASLD-control group (FIGURE 2A), whereas the animals in the MASLD-group had predominantly microvesicular steatosis along with macrovesicular steatosis of moderate intensity, with inflammatory activity and a mild degree of fibrosis (FIGURE 2B-C).…”

“…After acclimatization to the environment, the animals were randomized into three experimental groups according to their weight, as described in a previous publication (11) . An ALD-control group (ALD-control; n=8), 4-weeks alcohol group (ALC4; n=8); and 8-weeks alcohol group (ALC8; n=8).…”