Characterization of amphiphilic poly-N-vinylpyrrolidone derivatives by dynamic light scattering

Amphiphilic poly-N-vinylpyrrolidone nanocarriers with incorporated model proteins

“…The samples were filtered through 0.2 μm filters to remove the nonsolubilized pyrene, and the fluorescence intensity of the solubilized pyrene was measured at an excitation wavelength of 340 nm and emission wavelength of 385 nm using a Hitachi 650-10 S spectrophotometer (Hitachi Instruments Inc, Japan). The average size of polymeric nano-aggregates obtained in water and in physiological solution and their size distribution were determined by dynamic light scattering [31][32][33][34][35][36][37] at a temperature of 25 • C. A multibit, multi-τ full digital correlator (ALV-5000) was used that covered a dynamic range of about ten decades. For each sample, the mean diameter of six determinations was calculated by applying multimodal analysis.…”

Self-assembling nanoscaled drug delivery systems composed of amphiphilic poly-N-vinylpyrrolidones

“…The average size of polymeric nano-aggregates obtained in water and in physiological solution and their size distribution were determined by dynamic light-scattering [18][19][20]. The analysis was performed at a wavelength of 532 nm, at several scattering angles and at a temperature of 25°C.…”

Reprogramming of CaCo2 colorectal cancer cells after using the complex of poly-(N-vinylpyrrolidone) with small non-coding RNAs

“…An amphiphilic Poly-(N-vinylpyrrolidone), with an Mn = 3500, containing one hydrophobic n-octadecyl end group was used. The side groups of amino acid β-alanin were introduced in 8 mol % of polymer rings by the recently described method [[11], [12], [13], [14], [15], [16], [17], [18], [19], [20], [21], [22]]. The overage diameter of particles was approximately 100 nm.…”