Amphiphilic poly-N-vinylpyrrolidone nanocarriers with incorporated model proteins

Abstract

New nanoscaled polymeric carriers have been prepared on the basis of different amphiphilic water-soluble derivatives of poly-N-vinylpyrrolidone (PVP). The polymer self-assembly and interaction with model proteins (Bowman-Birk soybean proteinase inhibitor (BBI) and its hydrophobized derivatives) were studied in aqueous media. The possibility of inclusion of both BBI and hydrophobized oleic acid derivatives of BBI in amphiphilic PVP aggregates was investigated. It was ascertained that polymeric particles of size 50-80 nm were formed in certain concentrations of amphiphilic PVP and poorly soluble dioleic acid derivatives of BBI. Such polymeric aggregates are capable of solubilization of dioleoyl BBI with a concomitant prevention of its inactivation at low pH values.

Self‐assembled amphiphilic poly‐N‐vinylpyrrolidone nanoparticles as carriers for hydrophobic drugs: Stability aspects

“…This can be attributed to the strong hydrophobic interactions between the poorly soluble fluorescence probe and the nanoparticle core, resulting in compacting the particle structure to form smaller nanostructures. These results are in full agreement with data we previously obtained on the encapsulation of different hydrophobic drugs in Amph‐PVP nanoparticles . TEM and DLS studies also confirmed the spherical shape and narrow distribution of the pyrene‐loaded nanoparticles.…”

“…The PVP amide group easily forms hydrogen bonds and as a result, PVP has a high binding affinity for several small and large molecules . In our previous studies, we have shown that PVP amphiphilic derivatives form spherical nano‐scaled micelle‐like assemblies (30–200 nm) at considerably low concentrations in aqueous media, which can effectively incorporate various low‐molecular and macromolecular drugs . The characteristics of these vesicles and drug loading efficiency (LE) can be easily controlled by polymer design and nanoparticle preparation conditions .…”

Amphiphilic poly-N-vinylpyrrolidone nanoparticles as carriers for non-steroidal anti-inflammatory drugs: Characterization and in vitro controlled release of indomethacin

“…N-alkyl terminated (Amph-PVP) and di-n-alkyl terminated (Amph 2 -PVP) amphiphilic N-vinylpyrrolidone polymers were prepared using originally developed two-stage method as described in our previous studies (23,25). In the first stage, poly-Nvinylpyrrolidone (PVP) with one terminal carboxylic group was synthesized by free-radical polymerization of N-vinylpyrrolidone in the presence of initiator (azobisisobutyronitrile (AIBN) and chain-transmitter [mercaptoacetic acid (MAA)].…”

“…Since most drugs have a hydrophobic character, these drugs can be easily incorporated into the particle core by a covalent or a non-covalent bonding through hydrophobic interactions with the experimental methods such as direct dilution, dialysis (5), salting out procedure, or solvent evaporation method (24). In our previous study (25) we demonstrated the possibility of immobilization of model proteins (Bowman-Birk soybean proteinase inhibitor and its derivatives) on nano-scaled aggregates made of amphiphilic PVPs.…”

Modulation of Entrapment Efficiency and In Vitro Release Properties of BSA-Loaded Chitosan Microparticles Cross-Linked with Citric Acid as a Potential Protein–Drug Delivery System

“…The diverse delivery systems reported in the literature include liposomes, nanoparticles, microemulsions, and self-assembled polymeric nano and micro structures [6][7][8]. In this aspect, it has been previously shown that drug delivery systems based on amphiphilic poly-N-vinylpyrrolidone possess low toxicity, high biocompatibility, and stability, and can effectively entrap different low molecular weight and protein therapeutic agents [9][10][11][12][13].…”