Artemisinin is a sesquiterpene lactone with a peroxide bridge, and it is a natural anti-malarial drug obtained from Artemisia annua L. High germination percentages and rates are essential for commercial growers of this species to justify buying premium-priced seed to ensure high performance of their crop. The objective of our research was to determine the seed germination potential of A. annua variety 'CIM-Arogya.' An experiment was conducted using three constant (15 • C, 20 • C, and 25 • C) and three alternating (20/15 • C, 25/15 • C, and 20/25 • C) temperature regimes. Seed germination was evaluated in Petri dishes lined with filter paper under daily 16 h light and 8 h dark photoperiod. The highest estimates of mean percentage germination (82.0) and germination energy (20.5) were observed at 15 • C, followed by 20/15 • C (74.5% germination and 18.6% germination energy). Further, the 3 to 5-day period after seed sowing was ideal for the first germination count, and the 7 to 8-day period after sowing was best for final Temperature Effect on Seed Germination Potential in Artemisia annua L. 637 germination count. Day 7 was best for final germination determination at both constant and alternating temperature regimes. Thus, growers should sow seeds when the mean temperature is 15-20 • C for about 7 days.