Characterization of Altered BHK Cells Resistant to HVJ (Sendai Virus) Infection

Kimura, Yoshinobu; Aoki, Hiizu; Shimokata, Kaoru; Ito, Yasuhiko; Takano, Michiko; Maeno, Kazuo

doi:10.1099/0022-1317-47-1-193

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…Normally, the sendai virus binds to the cell surface sialic acid residues by way of F-glycoprotein, which mediates the fusion of the viral envelope with the cell plasma membrane at neutral pH. In addition, F-glycoprotein contains a ␤-galactose-terminated carbohydrate moiety, which binds to ASGPR at the hepatocyte plasma membrane (33)(34)(35). Based on these findings, we previously demonstrated, for the first time, gene transfer into porcine hepatocytes using the HVJ-liposome method to be feasible (36).…”

Section: Discussionmentioning

confidence: 99%

The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

Kawashita

Fujioka²,

Ohtsuru³

et al. 2005

Transplantation

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Section: Discussionmentioning

confidence: 99%

The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

Kawashita

Fujioka²,

Ohtsuru³

et al. 2005

Transplantation

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“…The resistance of BHK-R cells remained unchanged, even after prolonged incubation in virus-free maintenance medium under the conditions of no cell division. It was suggested that killing of virus-sensitive cells, which were generated during cell proliferation, was required for maintenance of the resistance.Altered baby hamster kidney (BHK-R) cells resistant to HVJ infection have been established and characterized (7,8). The BHK-R cells have been serially cultured in the presence of HVJ.…”

mentioning

confidence: 99%

“…Altered baby hamster kidney (BHK-R) cells resistant to HVJ infection have been established and characterized (7,8). The BHK-R cells have been serially cultured in the presence of HVJ.…”

mentioning

confidence: 99%

Effect of Cytolytic Infection on Maintenance of Resistance to HVJ (Sendai Virus) in an Altered BHK Cell Culture

Yokoo¹,

Miyadai

Yokochi

et al. 1987

Microbiology and Immunology

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Altered baby hamster kidney (BHK-R) cells were serially cultured in the continuous presence of hemagglutinating virus of Japan (HVJ). These cells showed a distinct resistance to superinfection with the homologous HVJ. This resistance of BHK-R cells gradually disappeared after serial passages in the presence of ultraviolet-irradiated HVJ particles which lost infectivity but still preserved hemagglutinating and neuraminidase activities. When BHK-R cells were serially cultured in the presence of a temperature-sensitive mutant of HVJ at non-permissive temperature, the cells also lost the resistance. The resistance of BHK-R cells remained unchanged, even after prolonged incubation in virus-free maintenance medium under the conditions of no cell division. It was suggested that killing of virus-sensitive cells, which were generated during cell proliferation, was required for maintenance of the resistance.Altered baby hamster kidney (BHK-R) cells resistant to HVJ infection have been established and characterized (7,8). The BHK-R cells have been serially cultured in the presence of HVJ. This cell line is distinguishable from persistentlyor transiently-infected cells by the absence of virus and virus-specific antigens. The resistance phenomenon observed in BHK-R cells is restricted to the homologous HVJ, and the cells remain susceptible to infection with other myxo-and paramyxoviruses. No interferon is involved in this resistance. The sialic acid content of BHK-R cells is approximately half that of normal BHK cells, and the process of adsorption and penetration of challenge virus HVJ is restrained. After serial passages in HVJ-free medium, BHK-R cells become susceptible to the HVJ infection with a corresponding increase in sialic acid content of the cells. It has been suggested that the neuraminidase of HVJ particles added to the culture seems to split of sialic acid residues present on the cell surface which are responsible for virus receptor function (1,2,10,14,15), resulting in the induction of the resistance. The present study was performed to obtain more detailed information on the interaction between cell membrane of BHK-R cells and HVJ particles.

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Reversible resistance to the antiviral action of interferon observed in altered BHK cells resistant to HVJ (Sendai virus) infection

Kimura

Yokochi

Miyadai

et al. 1984

Archives of Virology

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Altered baby hamster kidney (BHK-R) cells which were subcultured in the continuous presence of HVJ (hemagglutinating virus of Japan--the Sendai strain of parainfluenza 1 virus) showed a resistance to the antiviral action of both type I and II interferons. No evidence for a direct inactivation of interferon molecules during incubation of BHK-R cells was obtained. After serial subculture of BHK-R cells in growth medium free of HVJ, surface membranes with the proper sialic acid residues were restored and the cells became susceptible to the interferon action. It is suggested that binding sites for interferons might be ranked above HVJ receptors in the "receptor gradient".

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Characterization of Altered BHK Cells Resistant to HVJ (Sendai Virus) Infection

Cited by 6 publications

References 9 publications

The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

Effect of Cytolytic Infection on Maintenance of Resistance to HVJ (Sendai Virus) in an Altered BHK Cell Culture

Reversible resistance to the antiviral action of interferon observed in altered BHK cells resistant to HVJ (Sendai virus) infection

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