Characterization of .ALPHA.-L-arabinofuranosidase related to the secondary cell walls formation in Arabidopsis thaliana

Ichinose, Hitomi; Nishikubo, Nobuyuki; Demura, Taku; Kaneko, Satoshi

doi:10.5511/plantbiotechnology.27.259

Cited by 10 publications

(10 citation statements)

References 26 publications

(18 reference statements)

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“…Among them, AXAH I is able to hydrolyze arabinan (Lee et al ). Recently it has been shown that a recombinant family 51 enzyme from Arabidopsis (AtAraf51A, previously AtAsd1) possess a high activity against arabinoxylo‐oligosaccharides derived from arabinoxylan as a single functional α‐ l ‐arabinofuranosidase (Ichinose et al ) although it was reported as a bi‐functional α‐ l ‐arabinofuranosidase/β‐xylosidase (Minic et al ). Ichinose et al () also showed that AtAraf51A is able to hydrolyze arabinan.…”

Section: Discussionmentioning

confidence: 99%

“…Recently it has been shown that a recombinant family 51 enzyme from Arabidopsis (AtAraf51A, previously AtAsd1) possess a high activity against arabinoxylo‐oligosaccharides derived from arabinoxylan as a single functional α‐ l ‐arabinofuranosidase (Ichinose et al ) although it was reported as a bi‐functional α‐ l ‐arabinofuranosidase/β‐xylosidase (Minic et al ). Ichinose et al () also showed that AtAraf51A is able to hydrolyze arabinan. On the basis of the arabinose releasing activity of SlArf/Xyl2 against natural substrates, it is clear that SlArf/Xyl2 can act as an arabinoxylan arabinofuranohydrolase and/or arabinan hydrolase just like other family 51 α‐ l ‐arabinofuranosidases.…”

Section: Discussionmentioning

confidence: 99%

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Heterologous expression of tomato glycoside hydrolase family 3 α‐L‐arabinofuranosidase/β‐xylosidases in tobacco suspension cultured cells and synergic action of a family 51 isozyme under antisense suppression of the enzyme

Tateishi

Kamiyoshihara

Matsuno

et al. 2013

Physiologia Plantarum

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Four cDNA clones (SlArf/Xyl1-4) encoding α-l-arabinofuranosidase/β-xylosidase belonging to glycoside hydrolase family 3 were obtained from tomato (Solanum lycopersicum) fruit. SlArf/Xyl1 was expressed in various organs. Its level was particularly high in flower and leaves but low in fruit. SlArf/Xyl3 was highly expressed in flower. On the contrary, SlArf/Xyl2 and 4 were expressed in early developmental stage in various organs. Comparison with SlArf/Xyl4, SlArf/Xyl2 expression was observed in earlier stages. The active recombinant proteins were obtained by using BY-2 tobacco (Nicotiana tabacum) suspension cultured cells. The SlArf/Xyl1 and 2 recombinant proteins showed a bi-functional activity of α-l-arabinofuranosidase/β-xylosidase while the SlArf/Xyl4 protein possessed a β-xylosidase activity predominantly. Neither enzyme activities were detected for the SlArf/Xyl3 protein under the same conditions. Although SlArf/Xyl2 possessed a bi-functional activity, it preferentially hydrolyzed arabinosyl residues from tomato hemicellulosic polysaccharides. Antisense suppression of SlArf/Xyl2 resulted in no apparent changes in the enzyme activities, monosaccharide composition or fruit phenotype. Increment of a family 51 α-l-arabinofuranosidase expression rather than that of family 3 resulted in a restoring the activity in SlArf/Xyl2-suppressed fruit. The ability of recombinant SlArf/Xyl2 to hydrolyze both arabinan and arabinoxylan is nearly identical to that of α-l-arabinofuranosidases belonging to family 51. Our results suggested that BY-2 cells are a useful expression system for obtaining active cell wall hydrolyzing enzymes. In addition, an α-l-arabinofuranosidase activity derived from SlArf/Xyl2 would be essential in young organ development and the action of the enzyme could be restored by the other enzyme belonging to a different family under a defective condition.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Heterologous expression of tomato glycoside hydrolase family 3 α‐L‐arabinofuranosidase/β‐xylosidases in tobacco suspension cultured cells and synergic action of a family 51 isozyme under antisense suppression of the enzyme

Tateishi

Kamiyoshihara

Matsuno

et al. 2013

Physiologia Plantarum

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“…They play important roles in primary cell wall metabolism, particularly in the reassembly and degradation process (Girke et al 2004) and in secondary cell wall metabolism (Ichinose et al 2010). The Populus genome encodes seven GH10 xylanases, but only PttXyn10A had detectable ESTs in the wood-forming tissue (Geisler-Lee et al 2006) and was highly upregulated in different stages of wood formation (Aspeborg et al 2005;Takahashi-Schmidt 2008).…”

Section: Resultsmentioning

confidence: 99%

Association Genetics of Wood Physical Traits in the Conifer White Spruce and Relationships With Gene Expression

et al. 2011

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Marker-assisted selection holds promise for highly influencing tree breeding, especially for wood traits, by considerably reducing breeding cycles and increasing selection accuracy. In this study, we used a candidate gene approach to test for associations between 944 single-nucleotide polymorphism markers from 549 candidate genes and 25 wood quality traits in white spruce. A mixed-linear model approach, including a weak but nonsignificant population structure, was implemented for each marker-trait combination. Relatedness among individuals was controlled using a kinship matrix estimated either from the known half-sib structure or from the markers. Both additive and dominance effect models were tested. Between 8 and 21 single-nucleotide polymorphisms (SNPs) were found to be significantly associated (P # 0.01) with each of earlywood, latewood, or total wood traits. After controlling for multiple testing (Q # 0.10), 13 SNPs were still significant across as many genes belonging to different families, each accounting for between 3 and 5% of the phenotypic variance in 10 wood characters. Transcript accumulation was determined for genes containing SNPs associated with these traits. Significantly different transcript levels (P # 0.05) were found among the SNP genotypes of a 1-aminocyclopropane-1-carboxylate oxidase, a b-tonoplast intrinsic protein, and a long-chain acyl-CoA synthetase 9. These results should contribute toward the development of efficient marker-assisted selection in an economically important tree species.

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“…Mutant studies have been used successfully to demonstrate that arabinan is the endogenous substrate of some GH3s in Arabidopsis (Minic et al 2006;Arsovski et al 2009). The GH51 enzyme appears to be a clear-cut arabinofuranosidase that acts on both pectic arabinan and on xylan while playing a role in secondary wall deposition in Arabidopsis (Montes et al 2008;Ichinose et al 2010).…”

Section: Rg-i Side-chain Metabolismmentioning

confidence: 99%

Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

Guo

Runavot

Bourot

et al. 2019

Planta

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Characterization of .ALPHA.-L-arabinofuranosidase related to the secondary cell walls formation in Arabidopsis thaliana

Cited by 10 publications

References 26 publications

Heterologous expression of tomato glycoside hydrolase family 3 α‐L‐arabinofuranosidase/β‐xylosidases in tobacco suspension cultured cells and synergic action of a family 51 isozyme under antisense suppression of the enzyme

Heterologous expression of tomato glycoside hydrolase family 3 α‐L‐arabinofuranosidase/β‐xylosidases in tobacco suspension cultured cells and synergic action of a family 51 isozyme under antisense suppression of the enzyme

Association Genetics of Wood Physical Traits in the Conifer White Spruce and Relationships With Gene Expression

Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

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