Characterization of adhesive properties of red blood cells using surface acoustic wave induced flows for rapid diagnostics

Sivanantha, Ninnuja; Chung, Charles Wai; Collins, Dave; Şeşen, Muhsincan; Brenker, Jason; Coppel, Ross L.; Neild, Adrian; Alan, Tuncay

doi:10.1063/1.4895472

Cited by 41 publications

(42 citation statements)

References 36 publications

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“…, a temperature of 37°C and pH of 74) where was adhesion was favorable or detachment promoted. 192 Bussonnière et al 193 and Sivanantha et al 194 both applied this concept to use SAW-initiated cell de-adhesion as a method for cell separation and identification, with Bussonnière et al separating human embryonic kidney (HEK 293) cells from, smooth muscle cells (A7r5) in a droplet and Sivanantha et al applying the technique to healthy, treated, and malaria-infected red blood cells. The advantage with the SAW approach is that the method is inherently label-free and highly selective, without adversely affecting cell viability (Bussonnière et al measured apoptosis rates less than 5%).…”

Section: Saw-integrated Microfluidicsmentioning

confidence: 99%

Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

et al. 2017

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Surface acoustic waves (SAWs), are electro-mechanical waves that form on the surface of piezoelectric crystals. Because they are easy to construct and operate, SAW devices have proven to be versatile and powerful platforms for either direct chemical sensing or for upstream microfluidic processing and sample preparation. This review summarizes recent advances in the development of SAW devices for chemical sensing and analysis. The use of SAW techniques for chemical detection in both gaseous and liquid media is discussed, as well as recent fabrication advances that are pointing the way for the next generation of SAW sensors. Similarly, applications and progress in using SAW devices as microfluidic platforms are covered, ranging from atomization and mixing to new approaches to lysing and cell adhesion studies. Finally, potential new directions and perspectives on the field as it moves forward are offered, with a specific focus on potential strategies for making SAW technologies for bioanalytical applications.

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Section: Saw-integrated Microfluidicsmentioning

confidence: 99%

Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

et al. 2017

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“…While this is sufficient for many long-term cell culture studies, for other applications such as the trapping and analysis of rare cells it is desirable to dictate both the time and duration of capture in addition to the location of cell trapping. A number of active techniques have been used for particle and cell manipulation and patterning, including optical 14 15 , magnetic 16 , electrical 17 and acoustic 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 forces, although these differ in their suitability to the patterning of individual, isolated cells. High-frequency acoustic forces—where periodically fluctuating pressure conditions result in time-averaged forces that push suspended matter towards acoustic nodes/antinodes—are generally biocompatible and have demonstrated potential for long-term cell observation 22 .…”

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confidence: 99%

Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves

et al. 2015

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In single-cell analysis, cellular activity and parameters are assayed on an individual, rather than population-average basis. Essential to observing the activity of these cells over time is the ability to trap, pattern and retain them, for which previous single-cell-patterning work has principally made use of mechanical methods. While successful as a long-term cell-patterning strategy, these devices remain essentially single use. Here we introduce a new method for the patterning of multiple spatially separated single particles and cells using high-frequency acoustic fields with one cell per acoustic well. We characterize and demonstrate patterning for both a range of particle sizes and the capture and patterning of cells, including human lymphocytes and red blood cells infected by the malarial parasite Plasmodium falciparum. This ability is made possible by a hitherto unexplored regime where the acoustic wavelength is on the same order as the cell dimensions.

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“…32,37,42,43,[52][53][54][55] This narrow region was designed to be of a similar size to the width of the working section which coincides with the neck of the Y-junction. FIDTs consist of curved electrodes and they have been used widely in the literature to focus the ultrasonic power along a narrow region.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…The vibration can be induced in a number of ways, including the use of a resonating piezoelectric disk causing control of the size of bubble produced in a flow-focusing junction 25 or by use of surface acoustic waves (SAW). [37][38][39] In two phase microfluidic systems, SAW has been used for mixing, 40 control of droplet size, 41 individual droplet production, 42 droplet merging 43 and sorting droplets at single 44 and multiple Y-junctions. They have been used for particle concentration, 28,29 trajectory control 30,31 and sorting, 32,33 for atomization, 34,35 for sessile droplet displacement 36 and for manipulating cells.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic plug steering using surface acoustic waves

2015

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Digital microfluidic systems, in which isolated droplets are dispersed in a carrier medium, offer a method to study biological assays and chemical reactions highly efficiently. However, it's challenging to manipulate these droplets in closed microchannel devices. Here, we present a method to selectively steer plugs (droplets with diameters larger than the channel's width) at a specially designed Y-junction within a microfluidic chip. The method makes use of surface acoustic waves (SAWs) impinging on a multiphase interface in which an acoustic contrast is present. As a result, the liquid-liquid interface is subjected to acoustic radiation forces. These forces are exploited to steer plugs into selected branches of the Y-junction. Furthermore, the input power can be finely tuned to split a plug into two uneven plugs. The steering of plugs as a whole, based on plug volume and velocity is thoroughly characterized. The results indicate that there is a threshold plug volume after which the steering requires elevated electrical energy input. This plug steering method can easily be integrated to existing lab-on-a-chip devices and it offers a robust and active plug manipulation technique in closed microchannels.

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Characterization of adhesive properties of red blood cells using surface acoustic wave induced flows for rapid diagnostics

Cited by 41 publications

References 36 publications

Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves

Microfluidic plug steering using surface acoustic waves

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