Characterization of Acinetobacter type strains and isolates obtained from wastewater treatment plants by PCR fingerprinting

Wiedmann-Al-Ahmad, Margit; Tichy, Hans-Volker; Schön, G.

doi:10.1128/aem.60.11.4066-4071.1994

Cited by 77 publications

(19 citation statements)

References 28 publications

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“…have for long been described from various environmental sources. In 1994, Wiedman et al characterized for the first time A. lwoffii, A. junii , and A. johnsonii in wastewater treatment plants in Germany (Wiedmann-al-Ahmad et al, 1994 ). Later, Houang et al investigated soil samples from different areas in Hong Kong and showed that approximately 37% were positive for Acinetobacter spp.…”

Section: Natural Habitat Of Non- Baumannii Acinetobactermentioning

confidence: 99%

Reservoirs of Non-baumannii Acinetobacter Species

et al. 2016

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Acinetobacter spp. are ubiquitous gram negative and non-fermenting coccobacilli that have the ability to occupy several ecological niches including environment, animals and human. Among the different species, Acinetobacter baumannii has evolved as global pathogen causing wide range of infection. Since the implementation of molecular techniques, the habitat and the role of non-baumannii Acinetobacter in human infection have been elucidated. In addition, several new species have been described. In the present review, we summarize the recent data about the natural reservoir of non-baumannii Acinetobacter including the novel species that have been described for the first time from environmental sources and reported during the last years.

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Section: Natural Habitat Of Non- Baumannii Acinetobactermentioning

confidence: 99%

Reservoirs of Non-baumannii Acinetobacter Species

et al. 2016

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“…The FT‐IR analysis indicates that the 12 genomic species described by Bouvet and Jeanjean (1989) to be heterogenous because they are recovered throughout the DFA space. However, separation of the two genomic BG 8 and BG9 by FT‐IR was not surprising, because they can also be distinguished from each other when characterized by other techniques (Wiedmann‐Al‐Ahmad et al. 1994; Carr et al.…”

Section: Analysis Of the Known Genomic Species Of Acinetobacter By Ftmentioning

confidence: 99%

The rapid identification of Acinetobacter species using Fourier transform infrared spectroscopy

et al. 2004

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Aims: Fourier transform infrared (FT-IR) was used to analyse a selection of Acinetobacter isolates in order to determine if this approach could discriminate readily between the known genomic species of this genus and environmental isolates from activated sludge. Methods and Results: FT-IR spectroscopy is a rapid whole-organism fingerprinting method, typically taking only 10 s per sample, and generates ÔholisticÕ biochemical profiles (or ÔfingerprintsÕ) from biological materials. The cluster analysis produced by FT-IR was compared with previous polyphasic taxonomic studies on these isolates and with 16S-23S rDNA intergenic spacer region (ISR) fingerprinting presented in this paper. FT-IR and 16S-23S rDNA ISR analyses together indicate that some of the Acinetobacter genomic species are particularly heterogeneous and poorly defined, making characterization of the unknown environmental isolates with the genomic species difficult. Conclusions: Whilst the characterization of the isolates from activated sludge revealed by FT-IR and 16S-23S rDNA ISR were not directly comparable, the dendrogram produced from FT-IR data did correlate well with the outcomes of the other polyphasic taxonomic work (E.L. Carr, P. Kämpfer, B.K.C. Patel, V. Gürtler and R.J. Seviour, 2003, International Journal of Systematic and Evolutionary Microbiology 53, 953-963). Significance and Impact of the Study: We believe it would be advantageous to pursue this approach further and establish a comprehensive database of taxonomically well-defined Acinetobacter species to aid the identification of unknown strains. In this instance, FT-IR may provide the rapid identification method eagerly sought for the routine identification of Acinetobacter isolates from a wide range of environmental sources.

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“…With this method, genetic heterogeneity at the strain level can be detected [31–33]. The mixture of five random primers [34,35] yielded fingerprints of up to 12 bands between 2000 and 100 bp for the marine isolates. Banding patterns were reproducible if colonies of the same age were picked from the same medium.…”

Section: Resultsmentioning

confidence: 99%

The microbial diversity in picoplankton enrichment cultures: a molecular screening of marine isolates

Uphoff¹,

Felske²,

Fehr³

et al. 2001

FEMS Microbiology Ecology

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Picoplankton bacteria from a North Sea water sample were cultured under a variety of different conditions (nutrients, temperature, light, agitation, adhesion). Fluorescent in situ hybridization (FISH) analysis of the enrichments showed complex communities which were dominated by gamma-Proteobacteria or beta-Proteobacteria, followed by alpha-Proteobacteria and bacteria from the Cytophaga/Flavobacterium/Bacteroides (CFB) cluster. Among 410 isolates, a high degree of diversity was found, both with respect to colony color and morphology and with respect to genetic diversity. Isolated bacteria were classified into the main taxa by a special PCR approach, termed signature PCR (SIG-PCR). It was based on an oligo primer mixture targeting 16S rDNA which yielded PCR products of taxon-specific lengths. Again, gamma-Proteobacteria dominated (48%), followed by alpha-Proteobacteria (20%). beta-Proteobacteria were rarely isolated (eight strains of 410). The CFB cluster comprised the second largest phylum (14%), and 7.5% of all isolates belonged to the high-GC Gram-positives. Thus, isolated bacteria were representative of enrichment communities with the exception of the beta-Proteobacteria, which were detected in high abundance in certain enrichments by FISH but not isolated, and the high-GC Gram-positives, which were cultivated but not detected by FISH. A genomic fingerprinting technique, randomly amplified polymorphic DNA, showed that among 58 CFB isolates only 18 identical genotypes were found, and among the 84 alpha-Proteobacteria only eight identical genotypes were present. The data show the enormous diversity of cultivated bacteria from picoplankton enrichment cultures of one North Sea water sample, which is only a small fraction of the total picoplankton community.

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Characterization of Acinetobacter type strains and isolates obtained from wastewater treatment plants by PCR fingerprinting

Cited by 77 publications

References 28 publications

Reservoirs of Non-baumannii Acinetobacter Species

Reservoirs of Non-baumannii Acinetobacter Species

The rapid identification of Acinetobacter species using Fourier transform infrared spectroscopy

The microbial diversity in picoplankton enrichment cultures: a molecular screening of marine isolates

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