Characterization of acid-soluble collagen from the coelomic wall of Sipunculida

Su, Xiurong; Sun, Bei; Li, Yanyan; Hu, Qiu-Hui

doi:10.1016/j.foodhyd.2009.05.013

Cited by 16 publications

(11 citation statements)

References 27 publications

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“…The lace-like fibers might be related to the native helical structure of collagen, since the microstructure images of gelatin gels from the bovine bone showed a network with no fibers (Kittiphattanabawon et al, 2010a). The result was similar to those of the collagen extracted from the coelomic wall of Sipunculida (Su et al, 2009). Collagen sponge could serve as a fine drug carrier due to its network structure.…”

Section: Collagen Ultrastructuresupporting

confidence: 57%

Characterization of Acid-Soluble Collagen From Bone of Pacific Cod (Gadus macrocephalus)

Wang

Hou

et al. 2013

Journal of Aquatic Food Product Technology

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Acid-soluble collagen (ASC) was isolated from Pacific cod (Gadus macrocephalus) bone. The ASC was rich in glycine. The amount of imino acid was lower than that of calf skin collagen, as was the transition temperature (48.6 • C). Electrophoresis revealed two different α chains (α 1 and α 2 ), β-component, and γ -component. Fourier transform infrared spectroscopy measurement showed that ASC was in triple-helix structure. ASC had a solubility greater than 90% in a very acidic pH range (pH 1-4), and the solubility decreased with increasing NaCl concentrations up to 3%. Lyophilized ASC had a network ultrastructure with lace-like fibers, similar to calf skin collagen sponge.

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Section: Collagen Ultrastructuresupporting

confidence: 57%

Characterization of Acid-Soluble Collagen From Bone of Pacific Cod (Gadus macrocephalus)

Wang

Hou

et al. 2013

Journal of Aquatic Food Product Technology

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“…), the coelomic wall of Sipunculida (Su et al . ) and the scales of Lates calcarifer (Sankar et al . ).…”

Section: Resultsmentioning

confidence: 99%

“…; Su et al . ), and further studies should be carried out to demonstrate the functional properties of the collagen.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Acid-soluble Collagen from the Skin of Hammerhead Shark (Sphyrna lewini )

Chi

Wang

et al. 2013

Journal of Food Biochemistry

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The characteristics of the acid-soluble collagen (ASC) from the skin of hammerhead shark (Sphyrna lewini) (ASC-H) were investigated and compared with those of calf skin collagen (CSC). ASC-H with a yield of 4.23 ± 0.54% (based on the wet weight of skins) contained Gly (227 residues/1,000 residues) as the major amino acid and had imino acids of 205 residues/1,000 residues. Amino acid composition, sodium dodecyl sulfate polyacrylamide gel electrophoresis pattern and Fourier transform spectroscopy (FTIR), confirmed that ASC-H was mainly composed of type I collagen. The peptide map of ASC-H was different from that of CSC, suggesting the differences in amino acid sequences and conformation. Td of ASC-H was 16.89C, which was similar to those of cold-water fishes but significantly lower than those of tropical fish species and mammals. ASC-H exhibited high solubility in pH (1-4) and low NaCl concentrations (≤3%). In addition, the lyophilized collagen displayed loose, fibrous and porous ultrastructure. PRACTICAL APPLICATIONSAt present, large quantities of by-products, accounting for 50-70% of the original raw material, are generated during the process of aquatic products processing industry. Therefore, optimal use of these by-products is a promising way to protect the environment and produce value-added products to increase the revenue of fish processors. Recently, many scientists have focused their interests on isolation and characterization of collagens extracted from skins of aquatic organisms. However, no information regarding acid-soluble collagen (ASC) from skins of Hammerhead shark (Sphyrna lewini) has been reported. The aim of this study was to isolate and characterize ASC from skin of Hammerhead shark in comparison with type I collagen from calf skin (CSC). Therefore, the collagen extracted from the skin of hammerhead shark could bring considerably economic benefits as a substitute for mammalian collagen.

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“…We chose to use the Sircol collagen assay kit, which has been demonstrated to successfully quantify the collagen content in biological tissues in previous studies [42][43][44][45]. Other studies have used a hydroxyproline assay method but have followed very similar extraction procedures for the soluble and insoluble types of collagen [46][47][48][49].…”

Section: Discussionmentioning

confidence: 99%

Changes in the Mechanical and Biochemical Properties of Aortic Tissue due to Cold Storage

Chow

Zhang

2011

Journal of Surgical Research

110

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Characterization of acid-soluble collagen from the coelomic wall of Sipunculida

Cited by 16 publications

References 27 publications

Characterization of Acid-Soluble Collagen From Bone of Pacific Cod (Gadus macrocephalus)

Characterization of Acid-Soluble Collagen From Bone of Pacific Cod (Gadus macrocephalus)

Characterization of Acid-soluble Collagen from the Skin of Hammerhead Shark (Sphyrna lewini )

Changes in the Mechanical and Biochemical Properties of Aortic Tissue due to Cold Storage

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