Characterization of Acid-Soluble Collagen From Bone of Pacific Cod (Gadus macrocephalus)

Abstract

Acid-soluble collagen (ASC) was isolated from Pacific cod (Gadus macrocephalus) bone. The ASC was rich in glycine. The amount of imino acid was lower than that of calf skin collagen, as was the transition temperature (48.6 • C). Electrophoresis revealed two different α chains (α 1 and α 2 ), β-component, and γ -component. Fourier transform infrared spectroscopy measurement showed that ASC was in triple-helix structure. ASC had a solubility greater than 90% in a very acidic pH range (pH 1-4), and the solubility decreased with increasing NaCl concentrations up to 3%. Lyophilized ASC had a network ultrastructure with lace-like fibers, similar to calf skin collagen sponge.

Salt-Cured Atlantic Cod Skin: A Sustainable Source of Acid-Soluble Type I Collagen

“…As seen in the electron micrographs, the cod skin ASC exhibited this kind of morphology, not only calf skin type I collagen but acid-soluble collagen from Pacific cod bone [27]. Other authors reported similar structures for the silver catfish skin [22], sole fish skin [16] and Pacific cod bone [27] with the typical structure of type I ASC (flaky and porous). We related the lace-like fibres to the native helical structure of collagen, which reinforces our FTIR results.…”

“…Typically, random windings of coil-like structures are characteristic of the fibrous nature of the collagen [16]. As seen in the electron micrographs, the cod skin ASC exhibited this kind of morphology, not only calf skin type I collagen but acid-soluble collagen from Pacific cod bone [27]. Other authors reported similar structures for the silver catfish skin [22], sole fish skin [16] and Pacific cod bone [27] with the typical structure of type I ASC (flaky and porous).…”

In vitro assessment of the multifunctional bioactive potential of Alaska pollock skin collagen following simulated gastrointestinal digestion

“…Upgrading these co‐products not only provides the marine processing industry with a means for dealing with the high cost and legal restrictions associated with waste disposal but also provides a potential source of added‐value biofunctional ingredients . Collagens from fish species such as squid, walleye pollock, tuna and Pacific cod have been shown to be valuable sources of bioactive peptides. The properties of these bioactive peptide include antioxidant, immunomodulatory, metal‐chelating, angiotensin‐converting enzyme (ACE)‐inhibitory and DPP IV‐inhibitory activities.…”

Protective Effect of Cod (Gadus macrocephalus) Skin Collagen Peptides on Acetic Acid‐Induced Gastric Ulcer in Rats

“…The moisture, ash, fat, and protein contents of HMCCP and LMCCP were measured according to the AOAC (2000) methods no. 950.46, 920.153, 960.39, and 928.08 (Wang and others ). The purity of the samples was determined by detected their hydroxyproline content with spectrophotometry (Cao and Xu ).…”