Characterization of a β-1,4-mannanase from a newly isolated strain of Pholiota adiposa and its application for biomass pretreatment

Abstract

A highly efficient β-1,4-mannanase-secreting strain, Pholiota adiposa SKU0714, was isolated and identified on the basis of its morphological features and sequence analysis of internal transcribed spacer rDNA. P. adiposa β-1,4-mannanase was purified to homogeneity from P. adiposa culture supernatants by one-step chromatography on a Sephacryl gel filtration column. P. adiposa β-1,4-mannanase showed the highest activity toward locust bean gum (V max = 1,990 U/mg protein, K m = 0.12 mg/mL) ever reported. Its internal amino acid sequence showed homology with hydrolases from the glycoside hydrolase family 5 (GH5), indicating that the enzyme is a member of the GH5 family. The saccharification of commercial mannanase and P. adiposa β-1,4-mannanase-pretreated rice straw by Celluclast 1.5L (Novozymes) was compared. In comparison with the commercial Novo Mannaway(®) (113 mg/g-substrate), P. adiposa β-1,4-mannanase-pretreated rice straw released more reducing sugars (141 mg/g-substrate). These properties make P. adiposa β-1,4-mannanase a good candidate as a new commercial β-1,4-mannanase to improve biomass pretreatment.

Green synthesis and characterization of gold nanoparticles from Pholiota adiposa and their anticancer effects on hepatic carcinoma

“…PA possesses antimicrobial, anti-obesity, anti-hypertension, and hypolipidemic properties. These activities are attributed to the polysaccharides, ergosterol, and amino acids existing in PA (Ramachandran et al., 2014 ). A polysaccharide derived from PA functions as the primary physiologically active component.…”

Purification and characterisation of β‐mannanase from Lactobacillus plantarum (M24) and its applications in some fruit juices

“…The mannanase enzyme has been purified and characterised from different sources such as Streptomyces sp. (Takahashi et al, 1984), Bacillus subtilis (Mendoza et al, 1994;Zakaria et al, 1998), Sclerotium (Athelia) rolfsii (Grosswindhager et al, 1999), B. stearothermophilus (Mu et al, 2008), Aspergillus awamori (Kurakake & Komaki, 2001), Trichoderma harzianum (Ferreira & Filho, 2004) and Pholiota adiposa (Ramachandran et al, 2014).…”

The structural characterization of a polysaccharide exhibiting antitumor effect from Pholiota adiposa mycelia

“…adiposa are well known worldwide, including antitumor, antimicrobial, antifatigue, antihypertensive, free radical scavenging, and antihyperlipidemic activities. These medicinal functions are attributing to bioactive polysaccharides, amino acids, and ergosterol in the fruiting bodies or mycelia 2–4 . Especially, polysaccharide is a very important active ingredient in P .…”