Characterization of a Trypanosoma cruzi strain isolated from a non-endemic area in Northeast Brazil

Gomes, Yara de Miranda; Leal, Tereza Cristina Arcanjo; Silva, Marlene R.; Santiago, Christiane M. G.; Coutinho, Eridan M.

doi:10.1590/s0036-46651995000100014

Cited by 5 publications

(1 citation statement)

References 3 publications

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“…These results agree with those of other authors who have reported that more than 80% of naturally occurring strains have low or medium virulence for mice, as infectivity is an important parameter of virulence (Andrade 1974;Schlemper et al 1986;Carneiro et al 1991;Gomes et al 1995Gomes et al , 2003Steindel et al 1995;Fernandes et al 1997;Araújo et al 1999;Devera et al 2002;Toledo et al 2002).…”

Section: Discussionsupporting

confidence: 92%

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

et al. 2006

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In this paper, the infectivity for mice of Trypanosoma cruzi I and II strains isolated from sylvatic animals, triatomines, and humans is determined using fresh blood examination, hemoculture, culture of macerated organs, and polymerase chain reaction (PCR). Six strains were considered to have low infectivity (9.1-18.2%), five medium (27.3-45.4%), and one high (100.0%). Infectivity of T. cruzi strains isolated from sylvatic animals was significantly higher than that of strains isolated from humans and triatomines (p=0.0141). No significant difference was observed between the infectivity of T. cruzi I and II strains. The parasite was detected by fresh blood examination in one strain, by hemoculture and culture of macerated organs in four strains, and by PCR in all strains. We conclude that the infectivity is related to the host from which the strains were isolated, but the infectivity is not related to the genetic group of the parasite. We also conclude that the majority of the strains studied have low and medium infectivity for mice, and that PCR is an important tool to detect T. cruzi in strains with this biological characteristic.

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Section: Discussionsupporting

confidence: 92%

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

et al. 2006

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PCR and sero-diagnosis of chronic chagas’ disease biotechnological advances

Gomes

1997

Appl Biochem Biotechnol

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In the acute phase of Chagas' disease, when the parasitemia is high, diagnosis can be easily made using conventional parasitological methods. During the chronic phase, due to the low parasitemia, diagnosis is performed mainly by immunological methods. Conventional serological techniques are limited by cross-reactivity with other parasitic diseases, non-standardization of reagents, and the diversity of technical procedures. Methods are being developed to improve the sensitivity and specificity of diagnosis using molecular approaches. PCR-based detection systems and the use of recombinant antigens in ELISA are the most promising.

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Trypanosoma cruzi stocks isolated from acute Chagas disease patients lead to lethal murine infection

Oliveira

Stefani

Campos

et al. 1997

Transactions of the Royal Society of Tropical Medicine and Hygi

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Ten Trypanosoma cruzi stocks recently isolated from patients in acute and chronic phases of Chagas disease were inoculated to susceptible (A/Sn) mice. The mice were inoculated with 10(4) trypomastigotes intraperitoneally and monitored for parasitaemia and mortality for up to 300 d. The results demonstrated that (i) T. cruzi stocks isolated from patients in the acute phase killed animals, while stocks from patients in the chronic phase did not; (ii) survival curves differed statistically among mice infected with lethal stocks, and (iii) parasite burden did not affect the mortality rate of mice.

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Characterization of a Trypanosoma cruzi strain isolated from a non-endemic area in Northeast Brazil

Cited by 5 publications

References 3 publications

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

PCR and sero-diagnosis of chronic chagas’ disease biotechnological advances

Trypanosoma cruzi stocks isolated from acute Chagas disease patients lead to lethal murine infection

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