Characterization of a T7-Like Lytic Bacteriophage of Klebsiella pneumoniae B5055: A Potential Therapeutic Agent

Abstract

Characterization of bacteriophages to be used prophylactically or therapeutically is mandatory, as use of uncharacterized bacteriophages is considered as one of the major reasons of failure of phage therapy in preantibiotic era. In the present study, one lytic bacteriophage, KPO1K2, specific for Klebsiella pneumoniae B5055, with broad host range was selected for characterization. As shown by TEM, morphologically KPO1K2 possessed icosahedral head with pentagonal nature with apex to apex head diameter of about 39 nm. Presence of short noncontractile tail (10 nm) suggested its inclusion into family Podoviridae with a designation of T7-like lytic bacteriophage. The phage growth cycle with a latent period of 15 min and a burst size of approximately 140 plaque forming units per infected cell as well as a genome of 42 kbps and structural protein pattern of this bacteriophage further confirmed its T7-like characteristics. Phage was stable over a wide pH range of 4-11 and demonstrated maximum activity at 37 degrees C. After injection into mice, at 6 h, a high phage titer was seen in blood as well as in kidney and urinary bladder, though titers in kidney and urinary bladder were higher as compared to blood. Phage got cleared completely in 36 h from blood while from kidneys and urinary bladder its clearance was delayed. We propose the use of this characterized phage, KPO1K2, as a prophylactic/therapeutic agent especially for the treatment of catheter associated UTI caused by Klebsiella pneumoniae.

Bacteriophage PPST1 Isolated from Hospital Wastewater, A Potential Therapeutic Agent Against Drug Resistant Salmonella enterica subsp. enterica serovar Typhi

“…Moreover, some bacteriophages were reported to be lytic to bacteria in more than one genera. For example, phage KPO1K2 infected several strains of Klebsiella pneumoniae and Escherichia coli (Verma et al, 2009).…”

Penggunaan Bakteriofag untuk Kit Detektor Patogen Hawar Bakteri Kedelai

“…Pemanfaatan bakteriofag untuk deteksi suatu penyakit bakteri sangatlah efektif karena kespesifikan inang dari bakteriofag (Singh et al 2013). Pada siklus hidupnya, bakteriofag dapat mengakibatkan lisis pada bakteri inangnya yang ditandai dengan tidak adanya pertumbuhan koloni bakteri (Verma et al 2009). Pertumbuhan koloni bakteri pada medium biakan dapat ditunjukkan dengan terjadinya perubahan tingkat kemasaman.…”

Disrupting the mixed-species biofilm of Klebsiella pneumoniae B5055 and Pseudomonas aeruginosa PAO using bacteriophages alone or in combination with xylitol

“…It has a genome of 42 kb, a 10 nm short non-contractile tail and a T7-like structural protein pattern, suggesting its inclusion in the family Podoviridae with a designation of T7-like lytic bacteriophage. Depolymerase produced by the phage gave a turbid halo around a clear plaque on the bacterial lawn as a rseult of the degradation of capsular polysaccharide of K. Pneumoniae (Verma et al, 2009a).…”