Bacteriophage PPST1 Isolated from Hospital Wastewater, A Potential Therapeutic Agent Against Drug Resistant Salmonella enterica subsp. enterica serovar Typhi

Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat

doi:10.5772/30446

Cited by 5 publications

(7 citation statements)

References 21 publications

(22 reference statements)

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“…Double agar layer method was used to assay each dilution. Plaques were counted in the plate containing 0-3x10 2 plaques and counted as plaque forming units per milliliter (PFU/ml) (Rattanachaikunsopon & Phumkhachorn, 2007).…”

Section: Coliphages Titer Determinationmentioning

confidence: 99%

“…Each filtered lysate of the tested phages (ECP1, ECP2 and ECP3) was added to a log phase culture of EC 1 as an indicator strain at multiplicity of infectivity = 10 PFU/CFU/ml (Rattanachaikunsopon & Phumkhachorn, 2007). Samples of 1ml were withdrawn and filtered through membrane filters (0.45μm Millipore) after 4 min intervals and assayed by the double layer technique to determine the titer of non-adsorbed phages (Maillard et al, 1994).…”

Section: Adsorption Experimentsmentioning

confidence: 99%

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Differential Effects of Physical and Chemical Factors on Infectivity of Three Coliphages used as Water Quality Indicator

Samhan

Askora

Ezzat

et al. 2016

Egypt. J. Microbiol.

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HE EFFECT of temperature, pH, UV, petroleum ether (60-80), .......acetone (99 %), ethyl alcohol (95 %) and chloroform (99 %) on the infectivity of local coliphage isolates was studied. Three E.coli phages (ECP) were isolated from Ismailia Canal at Mostorod segment. Ability of local E.coli isolates to serve as a host was evaluated after storage at 4-6ºC, 25-28º C and 35-38º C for six months. E.coli isolate number 1 of seven tested served as host for phage reproduction and plaque formation (PF). Phage isolates were identified by plaque size and electron microscopy. ECP1 and ECP2 are Podoviridae and ECP3 is a Siphoviridae. The infectivity of coliphages decreased with increasing storage periods and incubation temperature. Exposure of somatic coliphages to acidic or alkaline pH, organic solvents or UV radiation (240 nm) or heating (≥ 60º C) diminished infectivity. Ability of E.coli1 to serve as a host for somatic coliphages inversely related to its age. No detectable effect was found for lower temperature ≤ 5º C on the infectivity of coliphage isolates during storage periods.

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Section: Coliphages Titer Determinationmentioning

confidence: 99%

Section: Adsorption Experimentsmentioning

confidence: 99%

Differential Effects of Physical and Chemical Factors on Infectivity of Three Coliphages used as Water Quality Indicator

Samhan

Askora

Ezzat

et al. 2016

Egypt. J. Microbiol.

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“…In another study, an isolated bacteriophage was evaluated for its sensitivity to different temperatures in the range of 50-90°C. The bacteriophage survived at 50-70°C, and its count decreased gradually with temperature; in other words, no bacteriophage was detected at 80-90°C 32 .…”

Section: Discussionmentioning

confidence: 92%

Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice

Dallal

Nikkhahi

Alimohammadi

et al. 2019

Rev. Soc. Bras. Med. Trop.

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Introduction: Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages. Methods: S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo. Results: The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages. Conclusions: The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis.

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“…They found that Salmonella-phages are stable at 27°C to 30°C for 30 minutes incubation and become unstable at 45°C, 55°C, and 60°C. Rattanachaikunsopon and Phumkhachorn (2012) also reported that phages would generally be inactive at 80°C. Furthermore, Basdew and Laing (2014) stated that high-temperature treatment could reduce phage activity.…”

Section: Discussionmentioning

confidence: 97%

“…Phage sensitivity to temperature, pH, and chlorine treatments was examined following the same procedure by Rattanachaikunsopon and Phumkhachorn (2012). The page sensitivity to temperature was monitored by giving various temperature treatments, i.e., 40°C, 50°C, 60°C, 70°C, 80°C, 90°C, and 100°C.…”

Section: Sensitivity To Temperature Ph and Chlorine Treatmentsmentioning

confidence: 99%

Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste

et al. 2020

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Abstract. Lingga R, Budiarti S, Rusmana I, Wahyu AT. 2020. Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste. Biodiversitas 21: 3234-3241. Escherichia coli is known as a pathogenic contaminant bacteria in hospital wastewater hazardous to humans and the environment. Concerns about the emergence of chlorine- and antibiotic-resistant bacteria increase the urgency to find an alternative strategy to control pathogenic bacteria in hospital wastewater. One of the alternatives is using lytic bacteriophage. This study aimed to isolate, characterize, and examine the efficacy of lytic bacteriophage against pathogenic Escherichia coli from hospital wastewater. It isolated, characterized (plaque morphology, host range, virion electron micrograph, and sensitivity to temperature, pH, and chlorine treatments), and tested the efficacy of lytic bacteriophages in controlling pathogenic E. coli isolated from hospital wastewater. Five phages were successfully obtained, all of which had clear plaques (lytic phage character). Based on host range assay, most of the phages could lyse all tested E. coli strains but not for other species. Electron micrograph photography revealed that the phages belonged to Myoviridae. The phages showed stability in high temperature, broad-ranged pH, and high concentrations of chlorine treatments. Assay on phages efficacy suggested that the phages are capable of significantly reducing the E. coli population both in sterilized and non-sterilized wastewater. The combination of phage treatment and chlorine was more effective than single phage treatment. The efficacy test revealed that phage application in wastewater had the best result seen from cocktail treatment and a combination of phage treatment and chlorine. These results suggested that the phage can be a potential candidate for disinfection purposes.

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Bacteriophage PPST1 Isolated from Hospital Wastewater, A Potential Therapeutic Agent Against Drug Resistant Salmonella enterica subsp. enterica serovar Typhi

Cited by 5 publications

References 21 publications

Differential Effects of Physical and Chemical Factors on Infectivity of Three Coliphages used as Water Quality Indicator

Differential Effects of Physical and Chemical Factors on Infectivity of Three Coliphages used as Water Quality Indicator

Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice

Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste

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