Characterization of a Soybean Leaf Protein That Is Related to the Seed Lectin and Is Increased with Pod Removal

Spilatro, Steven R.; Anderson, James M.

doi:10.1104/pp.90.4.1387

Cited by 23 publications

(12 citation statements)

References 27 publications

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“…heterodimers (Spilatro and Anderson, 1989). They are localized in the vacuoles of paraveinal mesophyll and bundle sheath cells of leaves (Franceschi and Giaquinta, 1983) and at lower levels in the leaf epidermis (Staswick, 1990).…”

Section: Introductionmentioning

confidence: 98%

Identification of a methyl jasmonate-responsive domain in the soybean vspB promoter.

1993

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Soybean vspB encodes a highly expressed vegetative storage protein-acid phosphatase. In soybean, vspB expression is stimulated by methyl jasmonate (MeJA) and sugars. The vspB promoter was studied by transforming tobacco with fusions of 5' noncoding vspB DNA and the gene encoding beta-glucuronidase (GUS). Constructs containing 833 bp of vspB 5' DNA showed high expression of GUS in stems, leaf veins and trichomes, sepals, and pollen. Sucrose (0.2 M) and MeJA (10(-5) M) increased gene expression when applied to leaf tissue. Deletion of the region -787 to -520 with respect to the transcription initiation site rendered the vspB promoter noninducible by MeJA but still sucrose responsive. This result indicates that DNA elements capable of modulating vspB by MeJA can be separated from carbon response elements. Further 5' end deletion from -520 to -403 or 3' end deletion from -165 to -289 removed DNA sequences involved in carbon modulation of gene expression. A DNA domain that mediates the MeJA response was further localized to a 50-bp region between -535 and -585. This domain when fused to a cauliflower mosaic virus (CaMV) 35S truncated (-88) promoter makes the CaMV promoter responsive to MeJA. The MeJA-responsive domain contains a G-box motif (CACGTG) and a C-rich sequence. A similar 50-bp DNA region is present in the putative promoter of vspA. Related sequences are located in a wound- and MeJA-responsive domain of the proteinase inhibitor II gene and a UV-responsive promoter domain of chs, the gene encoding chalcone synthase that is also responsive to MeJA.

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Section: Introductionmentioning

confidence: 98%

Identification of a methyl jasmonate-responsive domain in the soybean vspB promoter.

1993

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“…They exist as hetero-and homodimers [6,18,24]. A storage function for these proteins has been proposed because of their large accumulation in vacuoles of bundle sheath and paraveinal mesophyll cells of leaves when soybean plants are continually depodded [8,33,34].…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana Atvsp is homologous to soybean VspA and VspB, genes encoding vegetative storage protein acid phosphatases, and is regulated similarly by methyl jasmonate, wounding, sugars, light and phosphate

et al. 1995

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The soybean vegetative storage proteins, VSP alpha and VSP beta, are acid phosphatases that accumulate to very high levels in hypocotyls, young leaves and flowers and pods. The genes encoding the soybean VSP are activated by jasmonate, wounding, sugars and light and down regulated by phosphate and auxin. In this study, expression of an Arabidopsis thaliana gene (Atvsp) encoding a protein homologous to soybean Vsp alpha and Vsp beta, was examined and compared to expression of the soybean Vsp genes. Atvsp mRNA was present at high levels in flowers and buds and at low levels in roots, stems, leaves and siliques. Expression of Atvsp in leaves could be induced by wounding or by treatment of illuminated plants with methyl jasmonate and sucrose. Roots of plants with wounded leaves also accumulated Atvsp mRNA indicating that this gene can be regulated by a transmissible wound signal. Phosphate partially inhibited expression of Atvsp. Arabidopsis proteins of 29 and 30 kDa crossreacted with antibodies against soybean VSP. These proteins were very abundant in flowers and the proteins accumulated in leaves and roots of plants treated with methyl jasmonate. The level of these proteins in flowers was similar to the levels of soybean VSP in young soybean leaves. Overall, these data indicate that Arabidopsis Atvsp and soybean VspA/B genes are regulated similarly and that in both plants, the gene products can accumulate to high levels. This suggests that genes homologous to VspA/B may be of greater general significance than previously recognized.

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“…The VSP are glycoproteins that accumulate in leafparaveinal mesophyll and bundle sheath cells (10) and, in some situations, in epidermal cells (28). The 28-and 31-kD subunits, designated VSP-a and VSP-(, respectively, show 80% sequence identity but differ substantially in charge (16) and form homo-and heterodimers (24). Analysis of genomic clones indicated that the VSP are encoded by a small multigene family ofat least three members (20).…”

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confidence: 99%

Coregulation of Soybean Vegetative Storage Protein Gene Expression by Methyl Jasmonate and Soluble Sugars

Mason¹,

DeWald²,

Creelman³

et al. 1992

Plant Physiol.

110

106

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The soybean vegetative storage protein genes vspA and vspB are highly expressed in developing leaves, stems, flowers, and pods as compared with roots, seeds, and mature leaves and stems. In this paper, we report that physiological levels of methyl jasmonate (MeJA) and soluble sugars synergistically stimulate accumulation of vsp mRNAs. Treatment of excised mature soybean (Glycine max Merr. cv Williams) leaves with 0.2 molar sucrose and 10 micromolar MeJA caused a large accumulation of vsp mRNAs, whereas little accumulation occurred when these compounds were supplied separately. In soybean cell suspension cultures, the synergistic effect of sucrose and MeJA on the accumulation of vspB mRNA was maximal at 58 millimolar sucrose and was observed with fructose or glucose substituted for sucrose. In dark-grown soybean seedlings, the highest levels of vsp mRNAs occurred in the hypocotyl hook, which also contained high levels of MeJA and soluble sugars. Lower levels of vsp mRNAs, MeJA, and soluble sugars were found in the cotyledons, roots, and nongrowing regions of the stem. Wounding of mature soybean leaves induced a large accumulation of vsp mRNAs when wounded plants were incubated in the light. Wounded plants kept in the dark or illuminated plants sprayed with dichlorophenyidimethylurea, an inhibitor of photosynthetic electron transport, showed a greatly reduced accumulation of vsp mRNAs. The time courses for the accumulation of vsp mRNAs induced by wounding or sucrose/MeJA treatment were similar. These results strongly suggest that vsp expression is coregulated by endogenous levels of MeJA (or jasmonic acid) and soluble carbohydrate during normal vegetative development and in wounded leaves.We previously investigated changes in protein and mRNA populations that occur when soybean seedlings are exposed to water deficit (3,6,7,16,17). These studies showed that a 28-kD protein accumulates in the hypocotyl cell wall fraction when soybean seedlings are transplanted to low water potential soil (3,16). This protein and an immunologically related 3 1-kD protein are also abundant in soluble hypocotyl fractions (16) and localized in vacuoles (H.S. Mason, L.R. Griffing, and J.E. Mullet, unpublished data). Analysis of the cDNAs that encode the 28-and 3 1-kD proteins (16) revealed that they are identical with the soybean leaf VSP2 first iden-

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Characterization of a Soybean Leaf Protein That Is Related to the Seed Lectin and Is Increased with Pod Removal

Cited by 23 publications

References 27 publications

Identification of a methyl jasmonate-responsive domain in the soybean vspB promoter.

Identification of a methyl jasmonate-responsive domain in the soybean vspB promoter.

Arabidopsis thaliana Atvsp is homologous to soybean VspA and VspB, genes encoding vegetative storage protein acid phosphatases, and is regulated similarly by methyl jasmonate, wounding, sugars, light and phosphate

Coregulation of Soybean Vegetative Storage Protein Gene Expression by Methyl Jasmonate and Soluble Sugars

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