Identification of a methyl jasmonate-responsive domain in the soybean vspB promoter.

Abstract

Soybean vspB encodes a highly expressed vegetative storage protein-acid phosphatase. In soybean, vspB expression is stimulated by methyl jasmonate (MeJA) and sugars. The vspB promoter was studied by transforming tobacco with fusions of 5' noncoding vspB DNA and the gene encoding beta-glucuronidase (GUS). Constructs containing 833 bp of vspB 5' DNA showed high expression of GUS in stems, leaf veins and trichomes, sepals, and pollen. Sucrose (0.2 M) and MeJA (10(-5) M) increased gene expression when applied to leaf tissue. Deletion of the region -787 to -520 with respect to the transcription initiation site rendered the vspB promoter noninducible by MeJA but still sucrose responsive. This result indicates that DNA elements capable of modulating vspB by MeJA can be separated from carbon response elements. Further 5' end deletion from -520 to -403 or 3' end deletion from -165 to -289 removed DNA sequences involved in carbon modulation of gene expression. A DNA domain that mediates the MeJA response was further localized to a 50-bp region between -535 and -585. This domain when fused to a cauliflower mosaic virus (CaMV) 35S truncated (-88) promoter makes the CaMV promoter responsive to MeJA. The MeJA-responsive domain contains a G-box motif (CACGTG) and a C-rich sequence. A similar 50-bp DNA region is present in the putative promoter of vspA. Related sequences are located in a wound- and MeJA-responsive domain of the proteinase inhibitor II gene and a UV-responsive promoter domain of chs, the gene encoding chalcone synthase that is also responsive to MeJA.

Arabidopsis thalianaGH3.9 in Auxin and Jasmonate Cross Talk

“…22,23 To investigate whether GH3.9 expression in trichomes is regulated by JA, GH3.9 promoter::GUS transgenics were treated with exogenous MeJA (10 mM), resulting in a moderate increase in the GUS activity in the trichomes of young leaves (75% GUS positive) compared to untreated seedlings (54% GUS positive; Fig. 1B).…”

JIPs and RIPs: the regulation of plant gene expression by jasmonates in response to environmental cues and pathogens.

“…The promoter of vspB, which encodes a vegetative storage protein of soybean, contains a C-rich sequence and a G-box motif, separated by a stretch of 26 bp, that have recently been demonstrated to constitute a MeJA-responsive domain (Mason et al, 1993). C-rich motifs similar to that in vspBwere found in the soybean vspA promoter and also upstream of other wound-and jasmonate-inducible genes, such as those encoding phenylalanine ammonia-lyase and chalcone synthase (for references, see Mason et al, 1993). The promoter of the proteinase inhibitor II gene of potato also contains both C-rich and G-box sequences (Kim et al, 1992).…”

Comparative analysis of MIR168 promoters in three plant species

“…Despite an increasing number of reports predicting miRNAs and demonstrating their roles in the development and morphogenesis of plants, relatively little is known about the conservation of miR168 regulatory elements in various plant species (Han et al, 2014). It is generally accepted that the ACGT core element (ACE) is functionally important in a variety of promoters that respond to different stresses, such as light (Donald and Cashmore, 1990), jasmonic acid (Mason et al, 1993), and hormones stresses (Shen et al, 1993). The ACGT core sequences are found in Arabidopsis thaliana and also in rice (Mundy et al, 1990), wheat (Marcotte et al, 1989), and barley (Shen et al, 1996), suggesting that ACEs are conserved across plant species.…”