Characterization of a recombinant β-xylosidase of GH43 family fromBacteroides ovatusstrain ATCC 8483

Biochemical Characterization of Two Rhamnogalacturonan Lyases From Bacteroides ovatus ATCC 8483 With Preference for RG-I Substrates

“…The optimum temperature range for Bo3128 activity was found to be between 25 and 45 • C, whereas that for Bo4416 was between 30 and 50 • C (Figure 3C). This is similar to some previously reported enzymes from Bacteroides ovatus ATCC 8483, e.g., BoXyl43A at 35 • C (Zhou et al, 2020). The reason for their similar optimal temperature ranges may lie in the fact that Bacteroides ovatus is a member of the human intestinal bacteria, and the temperature of the human intestine is around 37 • C. In terms of thermostability, Bo3128 retained 50% activity after 2 h at 35 • C, making Bo3128 somewhat superior to Bo4416 that exhibited a half-life at 35 • C of only 50 min (Figure 3D).…”

Enzymatic hydrolysis of lignocellulosic biomass using a novel, thermotolerant recombinant xylosidase enzyme from Clostridium clariflavum: a potential addition for biofuel industry

“…Cu 2+ is known to catalyze the auto-oxidation of cysteine molecules, which cause the formation of inter and intra-molecular di-sulfide bonds or sulfenic acid and led to decreased enzyme production. 82 Zhou et al 83 characterized recombinant β-xylosidase enzyme from Bacteroides ovatus and showed its activation in the presence of Mg 2+ and Mn 2+ . Marcolongo et al 84 also reported β-xylosidase from Anoxybacillus sp.…”

Biochemical Basis of Xylooligosaccharide Utilisation by Gut Bacteria

“…The highest catalytic efficiency was observed for Bp-XylX-1, at 63 s −1 mM −1 ( Figure S5B and Table 1 ). The kinetics parameters of these β-xylosidases were better than other β-xylosidases from gut bacteria characterised to date on pNP-Xyl, such as Bacteroides ovatus ATCC 8483 (with K m and k cat /K m of 1.71 mM and 2.68 ± 0.08 s −1 mM −1 , respectively) [ 38 ], Bifidobacterium breve K-110 (with a K m of 0.87 mM) [ 39 ], or Bifidobacterium animalis subsp. lactis BB-12 (with K m and k cat of 15.6 ± 4.2 mM and 60.6 ± 10.8 s −1 , respectively) [ 40 ].…”