Characterization of a rat model of incisional pain

Brennan, Timothy J.; Vandermeulen, Erik; Gebhart, G. F.

doi:10.1016/0304-3959(95)01441-1

Cited by 957 publications

(883 citation statements)

References 19 publications

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“…Antisepsis of the plantar aspect of the right hind paw was made with a 10% povidone-iodine solution as described elsewhere (23). A 1-cm longitudinal incision was made with a surgical blade through the skin and fascia of the plantar region, starting 0.5 cm from the proximal edge of the heel.…”

Section: Subjects and Surgerymentioning

confidence: 99%

Comparison of pre- versus post-incision administration of intraplantar indomethacin and MK886 in a rat model of postoperative pain

Gaspar

Prado

2007

Braz J Med Biol Res

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The amplification of pain long after the initial stimulus may be avoided if the treatment of pain is introduced before its initiation. However, conflicting evidence exists about the efficacy of such preemptive analgesia for the management of postoperative pain. This study compares the efficacy of intraplantar administration of indomethacin (a non-selective inhibitor of cyclooxygenase) and MK886 (an inhibitor of 5-lipoxygenase-activating protein), separately or in combination to produce preemptive analgesia in a model of surgical incisional pain in male Wistar rats. All incised rats (5 to 6 rats per group) had allodynia at 2, 6, and 24 h after surgery as evaluated using von Frey filaments. MK886, but not indomethacin (50 to 200 µg/ paw), reduced the allodynia when injected either 1 h before or 1 h after surgery. The effect of preoperative MK886 (160 µg/paw) against incisional allodynia had a magnitude apparently similar to that produced by postoperative MK886. Pre-, but not postoperative MK886 (80 µg/paw) reduced the allodynia but the effect was seen only at 6 h after surgery. In contrast, MK886 (40 µg/paw) intensified the allodynia observed 2 h after the incision either injected before or after surgery. MK886 or indomethacin alone did not provide preemptive analgesia in the model of incisional pain. In contrast, the combination of MK886 with indomethacin reduced the allodynia more effectively when used before than after surgery, thus fulfilling the criteria for preemptive analgesia. In conclusion, preoperative inhibition of the local generation of both prostaglandins and leukotrienes by surgical incision may be an alternative to provide preemptive analgesia.

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Section: Subjects and Surgerymentioning

confidence: 99%

Comparison of pre- versus post-incision administration of intraplantar indomethacin and MK886 in a rat model of postoperative pain

Gaspar

Prado

2007

Braz J Med Biol Res

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“…The study also demonstrates that the antibiotics reduce the mechanical allodynia in a rat model of incisional pain. Incisional pain is characterized by a rapid and long-lasting mechanical allodynia and thermal hyperalgesia that correspond to those obtained during postsurgical states in humans (24). Therefore, the spinal administration of aminoglycoside antibiotics is effective against both phasic thermal and persistent incisional types of pain.…”

Section: Discussionmentioning

confidence: 99%

“…After hemostasis, the skin incision was closed with two 5-0 nylon sutures. After this procedure, anesthesia was discontinued and the animal allowed to recover in its home cage for a period of 2 h. Details of this model have been described elsewhere (24).…”

Section: Model Of Incisional Painmentioning

confidence: 99%

Antinociceptive potency of aminoglycoside antibiotics and magnesium chloride: a comparative study on models of phasic and incisional pain in rats

Prado

Filho

2002

Braz J Med Biol Res

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A close relationship exists between calcium concentration in the central nervous system and nociceptive processing. Aminoglycoside antibiotics and magnesium interact with N-and P/Q-type voltageoperated calcium channels. In the present study we compare the antinociceptive potency of intrathecal administration of aminoglycoside antibiotics and magnesium chloride in the tail-flick test and on incisional pain in rats, taken as models of phasic and persistent postsurgical pain, respectively. The order of potency in the tail-flick test was gentamicin (ED 50 = 3.34 µg; confidence limits 2.65 and 4.2) > streptomycin (5.68 µg; 3.76 and 8.57) = neomycin (9.22 µg; 6.98 and 12.17) > magnesium (19.49 µg; 11.46 and 33.13). The order of potency to reduce incisional pain was gentamicin (ED 50 = 2.06 µg; confidence limits 1.46 and 2.9) > streptomycin (47.86 µg; 26.3 and 87.1) = neomycin (83.17 µg; 51.6 and 133.9). The dose-response curves for each test did not deviate significantly from parallelism. We conclude that neomycin and streptomycin are more potent against phasic pain than against persistent pain, whereas gentamicin is equipotent against both types of pain. Magnesium was less potent than the antibiotics and effective in the tail-flick test only.

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“…Withadraw threshold was measured with von Frey filaments 6 as suggested by Brennan et al (1996). Each filament, in a crescent order was applied three consecutive times with a 3 to 5 seconds interval and if no response was observed, another filament was tested.…”

Section: Methodsmentioning

confidence: 99%

“…Postoperatory pain simulation in laboratory animals include, among others, plantar incision, developed by Brennan et al (1996), and formalin test, presented by Dubuisson & Dennis (1977). Formalin tests have been used to simulate postoperatory inflammatory component and presents two phases: phase I with peak at 5 minutes mediated by local sensitive fibers; and phase II, about 15 minutes after injection, mediated by inflammatory mediators and non noxious sensitive fibers (Bhutta et al 2001, Hogan 2002, Ashmawi et al 2003.…”

Section: Introductionmentioning

confidence: 99%