Abstract:A survey conducted in pepper-growing tracts of Karnataka State, covering 165 fields in 33 villages, revealed the occurrence of many pepper mosaic diseases. Based on reactions on selected test plants, the viruses were identified as pepper vein banding virus (PVBV), pepper veinal mottle virus, potato virus Y, cucumber mosaic virus, and tobacco mosaic virus. Among these, PVBV was the most prevalent. PVBV was purified from infected leaves of Capsicum annuum cv. California Wonder. Electron microscopy revealed flexu… Show more
“…This finding is in agreement with Ravi et al, (1997) who compared the N-terminal sequence of PVBV CP with all the potyviral CP sequences and observed the DAG sequence conserved in all aphid-transmitted Potyviruses in the N-terminal sequence of the PVBV CP. Similar observations of conserved DAG motif was also made by Tsai et al, (2008) in all ChiVMV isolates including Indian isolate.…”
Section: Pcr Analysis Of Murda Complex Infected Chilli Plants For Potsupporting
“…This finding is in agreement with Ravi et al, (1997) who compared the N-terminal sequence of PVBV CP with all the potyviral CP sequences and observed the DAG sequence conserved in all aphid-transmitted Potyviruses in the N-terminal sequence of the PVBV CP. Similar observations of conserved DAG motif was also made by Tsai et al, (2008) in all ChiVMV isolates including Indian isolate.…”
Section: Pcr Analysis Of Murda Complex Infected Chilli Plants For Potsupporting
“…The current study confirmed the incidence of ChiVMV in Naga chilli. The incidence of ChiVMV in C. annum was reported from South India [1,4,6]. To the best of our knowledge, this is the first report and molecular evidence of ChiVMV infection in Naga chilli from North-east India.…”
The present study confirms the occurrence of Chilli veinal mottle virus (ChiVMV) under the genus Potyvirus in Naga chilli (Capsicum chinense) in Meghalaya based on mechanical transmission assay, transmission electron microscopy, RT-PCR and sequence analysis. This is the first record of Chivmv in Naga chilli in North-East India.
“…Earlier, we had reported the purification and characterization of PVBV and showed that it was a distinct member of the genus Potyvirus [16,23]. The NIa protease of PVBV showed 50 and 47% identity with TuMV and TEV NIa protease, respectively.…”
Summary. The nuclear inclusion protein a (NIa) protease plays an important role in the life cycle of potyviruses by processing the viral polyprotein into functional proteins. For functional characterization, the NIa protease from Pepper vein banding potyvirus (PVBV) was overexpressed in Escherichia coli and purified. Using a recombinant polyprotein substrate containing the nuclear inclusion protein b (NIb)-coat protein (CP) cleavage site, a trans-cleavage assay was developed for the NIa protease. The polyprotein substrate also possessed the cleavage site between NIa and NIb, in addition to the NIb-CP site. However, no trans-cleavage by the NIa protease between NIa and NIb was detected indicating that the cleavage between NIa and NIb under natural conditions would be by a cis-cleavage reaction. Site-specific mutations of the conserved residues D81, D90, C110, T146, C151 and H167 were performed to investigate their roles in the catalytic process of the protease. Such an analysis has revealed that D81 and C151 constitute two of the catalytic triad residues in the NIa protease, D90 and C110 are not essential for catalysis, and T146 and H167 are probably involved in binding to Gln at the P1 position of the substrate.
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