Characterization of a novel strain phylogenetically related to Kocuria rhizophila and its chemical modification to improve performance of microbial fuel cells

Luo, Jianmei; Li, Ming; Zhou, Minghua; Hu, Youshuang

doi:10.1016/j.bios.2015.02.025

Cited by 41 publications

(15 citation statements)

References 71 publications

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“…All 15 isolates of Fe(III)-reducing bacteria were confirmed to have exoelectrogenic ability by the polarization test, which is one of the most widely used techniques to determine the bioelectrochemical activity of exoelectrogenic bacteria and to test MFCs performance (Puig et al, 2010; Luo et al, 2015). Most exoelectrogenic bacteria isolates are Gram-negative and belong to the phylum Proteobacteria (Zhi et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…However, acetate exhibits higher coulombic efficiency than glucose and thus it has become the most widely applied substrate (Pham et al, 2003; Zuo et al, 2008; Fu et al, 2013). Nevertheless, some exoelectrogenic bacteria do not utilize acetate as an optimal carbon substrate (Xu and Liu, 2011; Feng et al, 2014; Luo et al, 2015), and some cannot metabolize acetate at all. For example, Shewanella oneidensis oxidizes lactate rather than acetate under anaerobic conditions (Lovley et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Electricity Generated by Soil in Microbial Fuel Cells and the Isolation of Soil Source Exoelectrogenic Bacteria

et al. 2016

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Soil has been used to generate electrical power in microbial fuel cells (MFCs) and exhibited several potential applications. This study aimed to reveal the effect of soil properties on the generated electricity and the diversity of soil source exoelectrogenic bacteria. Seven soil samples were collected across China and packed into air-cathode MFCs to generate electricity over a 270 days period. The Fe(III)-reducing bacteria in soil were enriched and sequenced by Illumina pyrosequencing. Culturable strains of Fe(III)-reducing bacteria were isolated and identified phylogenetically. Their exoelectrogenic ability was evaluated by polarization measurement. The results showed that soils with higher organic carbon (OC) content but lower soil pH generated higher peak voltage and charge. The sequencing of Fe(III)-reducing bacteria showed that Clostridia were dominant in all soil samples. At the family level, Clostridiales Family XI incertae sedis were dominant in soils with lower OC content but higher pH (>8), while Clostridiaceae, Lachnospiraceae, and Planococcaceae were dominant in soils with higher OC content but lower pH. The isolated culturable strains were allied phylogenetically to 15 different species, of which 11 were Clostridium. The others were Robinsoniella peoriensis, Hydrogenoanaerobacterium saccharovorans, Eubacterium contortum, and Oscillibacter ruminantium. The maximum power density generated by the isolates in the MFCs ranged from 16.4 to 28.6 mW m-2. We concluded that soil OC content had the most important effect on power generation and that the Clostridiaceae were the dominant exoelectrogenic bacterial group in soil. This study might lead to the discovery of more soil source exoelectrogenic bacteria species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of Electricity Generated by Soil in Microbial Fuel Cells and the Isolation of Soil Source Exoelectrogenic Bacteria

et al. 2016

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“…EABs have a major importance in MFCs as they catalyzes the oxidation of organic substrates to generate power [ 46 ]. According to a previous study, EABs are responsible for voltage generation and electron transfer in SMFCs [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

A Novel Early Warning System Based on a Sediment Microbial Fuel Cell for In Situ and Real Time Hexavalent Chromium Detection in Industrial Wastewater

Zhao

Liu

Niu

et al. 2018

Sensors

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Hexavalent chromium (Cr(VI)) is a well-known toxic heavy metal in industrial wastewater, but in situ and real time monitoring cannot be achieved by current methods used during industrial wastewater treatment processes. In this study, a Sediment Microbial Fuel Cell (SMFC) was used as a biosensor for in situ real-time monitoring of Cr(VI), which was the organic substrate is oxidized in the anode and Cr(VI) is reduced at the cathode simultaneously. The pH 6.4 and temperature 25 °C were optimal conditions for the operation. Under the optimal conditions, linearity (R2 = 0.9935) of the generated voltage was observed in the Cr(VI) concentration range from 0.2 to 0.7 mg/L. The system showed high specificity for Cr(VI), as other co-existing ions such as Cu2+, Zn2+, and Pb2+ did not interfere with Cr(VI) detection. In addition, when the sediment MFC-based biosensor was applied for measuring Cr(VI) in actual wastewater samples, a low deviation (<8%) was obtained, which indicated its potential as a reliable biosensor device. MiSeq sequencing results showed that electrochemically active bacteria (Geobacter and Pseudomonas) were enriched at least two-fold on the biofilm of the anode in the biosensor as compared to the SMFC without Cr(VI). Cyclic voltammetry curves indicated that a pair of oxidation/reduction peaks appeared at −111 mV and 581 mV, respectively. These results demonstrated that the proposed sediment microbial fuel cell-based biosensor can be applied as an early warning device for real time in situ detection of Cr(VI) in industrial wastewaters.

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“…This method was also applied to E. coli [57]. Recently, it has been reported that lysozyme treatment increased 1.75-fold of the MFC performance with K. rhizophila P2-A-5 [58]. Thus, chemical treatment is one of the important approaches to modify the microbial cells for the improvement of the MFC performance.…”

Section: Chemical Treatmentmentioning

confidence: 99%

Catalyst Development of Microbial Fuel Cells for Renewable-Energy Production

Azuma¹,

Ojima²

2019

Current Topics in Biochemical Engineering

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In this chapter, we focus on microbial fuel cells (MFCs) that convert the energy from organic matters into electrical energy using microorganisms. MFCs are greatly expected to be used as a relatively low-cost and safe device for generating renewable energy using waste biomass as a raw material. At present, however, it has not reached the desired practical application because of the low-power generation; hence, improvements on fuel cell efficiency, such as electrode materials, are still being examined. Here, we focus on the microorganisms that can be used as catalysts and play a central role in improving the efficiency of the fuel cells. Several kinds of microbial catalysts are used in MFCs. For example, Shewanella oneidensis has been well studied, and as known, since S. oneidensis transports the electrons generated within the cell to the surface layer, it does not require a mediator to pass the electrons from the cells to the electrode. Furthermore, Escherichia coli and Saccharomyces cerevisiae, a model organism for MFCs, are also used. The improvements of such microbial catalysts have also been proceeding actively. Here, we elaborated on the principle of MFCs as well as the current situation and latest research on the catalyst development.

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Characterization of a novel strain phylogenetically related to Kocuria rhizophila and its chemical modification to improve performance of microbial fuel cells

Cited by 41 publications

References 71 publications

Characterization of Electricity Generated by Soil in Microbial Fuel Cells and the Isolation of Soil Source Exoelectrogenic Bacteria

Characterization of Electricity Generated by Soil in Microbial Fuel Cells and the Isolation of Soil Source Exoelectrogenic Bacteria

A Novel Early Warning System Based on a Sediment Microbial Fuel Cell for In Situ and Real Time Hexavalent Chromium Detection in Industrial Wastewater

Catalyst Development of Microbial Fuel Cells for Renewable-Energy Production

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